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Machines on genes: enzymes that make, break, and move DNA and RNA

12—14 August 2009

Robinson College, Cambridge, UK



Organizers:
Ben Luisi (Cambridge University, UK)
Marshall Stark (University of Glasgow, UK)
Richard Bowater (University of East Anglia, UK)
Wednesday 12 August 2009
12:30 - 14:00 Registration with lunch

14:00 - 14:30
V(D)J recombination: from regulation to 3D assembly
Wei Yang (National Institutes of Health, USA)
14:30 - 15:00
How do helicases know which way to walk?
Dale Wigley (Cancer Research, UK)
15:00 - 15:20
Assembly of a machine for concurrent action at eight phosphodiester bonds
Selected oral communication - Jacqui Marshall (University of Bristol, UK)
15:20 - 15:50 Coffee/tea break

15:50 - 16:20
Chromatin structure and regulation of its compaction
Daniela Rhodes (University of Cambridge, UK)
16:20 - 16:50
Single-molecule techniques for monitoring topology, transcription, and replication
Nynke Dekker (Delft University of Technology, The Netherlands)
16:50 - 17:10
Mass Spectrometry reveals the subunit architecture of eukaryotic RNA polymerases I and III
Selected oral communication - Laura Lane (University of Cambridge, UK)
17:10 - 18:30 Drinks reception with poster session one (odd numbers)

18:30 - 20:00 Dinner

20:15 - 20:45
Induced fit: a common strategy used by the ribosome in decoding , peptidyl transferase and termination
Venki Ramakrishnan (MRC Laboratory of Molecular Biology, UK)
Thursday 13 August 2009
09:00 - 09:30
Orchestrating serine resolvases
Phoebe Rice (The University of Chicago, USA)
09:30 - 10:00
Site-specific recombination by ƒÖC31 integrase
Maggie Smith (University of Aberdeen, UK)
10:00 - 10:20
A single-strand cleavage reaction provides insight into the catalytic mechanism of Tn3 resolvase
Selected oral communication - Femi Olorunniji (University of Glasgow, UK)
10:20 - 10:40
Identification of an ancient RNA machine for protein biosynthesis within the contemporary ribosome
Selected oral communication - Ada Yonath (Weizmann Institute, Israel)
10:40 - 11:10 Coffee/tea break

11:10 - 11:40
The anatomy and action of molecular machines that replicate and segregate DNA
David Sherratt (University of Oxford, UK)
11:40 - 12:00
High-throughput structure/function analysis of the nucleotide addition cycle of RNA polymerase
Selected oral communication - Robert Weinzierl (Imperial College, London, UK)
12:00 - 12:20
Substrate recognition and catalysis by flap endonucleases
Selected oral communication - Jane Grasby (University of Sheffield, UK)
12:20 - 14:00 Lunch with poster session two (even numbers)

14:00 - 14:30
Lesion recognition in DNA replication and repair
Lorena Beese (Duke University, USA)
14:30 - 15:00
DNA topoisomerases: coupling ATP hydrolysis to DNA strand passage
Tony Maxwell (John Innes Centre, Norwich, UK)
15:00 - 15:20
A re-appraisal of the role of ATP in type II topoisomerases
Selected oral communication - Andy Bates (University of Liverpool, UK)
15:20 - 15:40 Coffee/tea break

15:40 - 16:10
Polymerase-mediated orchestration of DNA double-strand break repair processes
Aidan Doherty (University of Sussex, UK)
16:10 - 19:00 Free time

19:00 - 20:30 Conference Dinner

Friday 14 August 2009
09:30 - 10:00
Tales of transposons: machines that move DNA
Nancy Craig (Johns Hopkins School of Medicine, USA)
10:00 - 10:30
Recognition of branched DNA structure by junction-resolving enzymes
David Lilley (University of Dundee, UK)
10:30 - 11:00 Coffee/tea break

11:00 - 11:20
Using linked multimers to understand the action of the FtsK DNA translocase
Selected oral communication - Estelle Crozat (University of Oxford, UK)
11:20 - 11:40
Crystal structures of human RECQ1 protein in complexes with DNA and ADP
Selected oral communication - Opher Gileadi (University of Oxford, UK)
11:40 - 12:10
Ticket to slide: energy-efficient motion on DNA by a helicase
Mark Szczelkun (University of Bristol, UK)
12:10 - 12:30 Meeting close - packed lunch on departure