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Enzyme mechanisms: fast reaction and computational approaches

9—10 October 2008

Manchester Interdisciplinary Biocentre, UK



9 October 2008

Poster Session 1: 12:30-14:00


P001 PcrA helicase ATPase mechanism
C.P. Toseland, A.F. Slatter, J.L. Hunter and M.R. Webb
P003 Solvent isotope effect on dihydrofolate reductase catalysis
E.J. Loveridge and R.K. Allemann
P005 Choreographing radical propagation in a B6 and B12 enzyme
K. Wolthers, S. Rigby and N. Scrutton
P007 Magnetic field effects in adenosylcobalamin-dependent enzymes
A.R. Jones, J.R. Woodward and N.S. Scrutton
P009 Directed evolution of PETN reductase to improve utility as a REDOX biocatalyst
M.E. Hulley, H.S. Toogood, G.M. Stephens and N.S. Scrutton
P011 Structure-based mechanism of CMP-Kdo synthetase: unusual Kdo-dependent activation of the CTP a-phosphate to act as general base
C. Levy, D.J. Heyes, N.S. Scrutton and D. Leys
P013 Kinetic analysis of two mutants of the FAD/NADPH binding domain of human methionine synthase reductase
X. Lou, K.R. Wolthers and N.S. Scrutton
P015 Use of computational chemistry techniques to assign chemical species from the reductive half-reaction of AADH
J. Pang, S. de Visser, M. Sutcliffe and N. Scrutton
P017 Initial characterisation of a temperature-adapted old yellow enzyme homologue
B.V. Adalbjornsson, H.S. Toogood and N.S. Scrutton
P019 An internal reaction chamber in dimethylglycine oxidase provides efficient protection from exposure to toxic formaldehyde: Part I.
T. Tralau, P. Lafite, N.S. Scrutton and D. Leys
P021 pH dependence of kinetic isotope effects in monoamine oxidase A
R. Dunn, A. Munro and N. Scrutton

10 October 2008

Poster Session 2: 13:00-14:00


P002 Fluorescence stopped-flow studies of conformational changes in DNA repair enzymes during the catalytic cycle
O. Fedorova, N. Kuznetsov, V. Koval, G. Nevinsky, D. Zharkov and K. Douglas
P004 Conformational dynamics in the cytochrome P450 BM3/N-palmitoylglycine complex: a temperature-jump spectroscopy study on the proposed proximal-distal transition
S. Brenner, S. Hay, H.M. Girvan, A.W. Munro and N.S. Scrutton
P006 Reduction of diflavin enzymes by photoexcitable thiouredopyrene-3,6,8-trisulfonate (TUPS)
D.J. Heyes, A.-M. Quinn, P. Cullis, M. Lee, A.W. Munro and N.S. Scrutton
P008 Mutation induces multiple reactive configurations in an enzyme-coenzyme complex which inter-convert at high-pressure
C.R. Pudney, S. Hay, P. Lafite, D. Leys and N.S. Scrutton
P010 Hydrostatic pressure: a new probe of promoting motions in enzyme catalysis
S. Hay and N.S. Scrutton
P012 QM/MM study of the sulfoxidation reaction of cytochrome P450
C.S. Porro, M.J. Sutcliffe and S.P. de Visser
P014 Enzymic H-tunnelling – a role for promoting vibrations?
L.O. Johannissen, S. Hay, M.J. Sutcliffe and N.S. Scrutton
P016 The ground and excited state dynamics in the light-driven enzyme protochlorophyllide oxidoreductase is anchored by the multiple roles of active site Tyr and Lys residues
B.R.K. Menon, D.J. Heyes and N.S. Scrutton
P018 Kinetics and mechanism of CB1954 reduction by E. coli nitroreductase
A. Christofferson, D. Jarrom, E. Hyde, M. Jaberipour, S. Vass, C. Guise, P.F. Searle, S. Daff, M.R. Hicks, S.A. White and J. Wilkie
P020 An internal reaction chamber in dimethylglycine oxidase provides efficient protection from exposure to toxic formaldehyde: Part II.
P. Lafite, T. Tralau, N.S. Scrutton and D. Leys
P022 Oxacarbenium ion intermediates in enzyme catalyzed glycoside hydrolysis
I.R. Gre