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Analysis of free radicals, radical modifications and redox signalling

18—19 April 2011

Aston University, Birmingham, UK



Meeting background

The importance of free radicals in biological systems has been recognized for many decades but until recently, was largely viewed as a deleterious aspect of inflammation and toxicity. However, strong evidence has now emerged that many radical modifications to biomolecules are reversible and have a regulatory role. In particular, the susceptibility of proteins for rapid and reversible free radical-mediated alterations in structure and function allows them to act as signalling intermediates that are sensitive to redox environment.
 

Organizers:
Corinne Spickett (Aston University, Birmingham, UK)
Helen Griffiths (University of Aston, Birmingham, UK)
Paul Winyard (University of Exeter, UK)

Science Programme

 

Monday 18 April 2011
09:30 - 10:00 Registration with coffee/tea

10:00 - 10:20
Welcome, housekeeping and session introduction
Helen Griffiths (University of Aston, Birmingham, UK)

Measuring reactive oxygen species and nitrogen species

 

Chairs:
Helen Griffiths (Aston University, Birmingham, UK)
Balaraman Kalyanaraman (University Medical College of Wisconsin, USA)
Monday 18 April 2011
10:20 - 11:00
Global profiling of reactive oxygen and nitrogen species in biological systems: Are we there yet?
Balaraman Kalyanaraman (University Medical College of Wisconsin, USA)
11:00 - 11:30 Coffee/tea break

11:30 - 12:10
Do oxidative damage or defective redox signalling contribute to age-related loss of skeletal muscle?
Malcolm Jackson (University of Liverpool, UK)
12:10 - 12:30
Analysis of ROS production in brown fat mitochondria using hydrogen peroxide- and superoxide-sensitive probes
Selected oral communication - Irina Shabalina (Stockholm University, Sweden)
12:30 - 14:00 Lunch (with poster session)

14:00 - 14:40
Measurement and meaning of biomarkers of oxidative and nitrative stress in human inflammatory joint disease
Paul Winyard (University of Exeter, UK)
14:40 - 15:00
Assessment of oxidative stress in lymphocytes following exercise
Selected oral communication - James Turner (University of Birmingham, UK)
15:00 - 15:20
Spectroscopic analysis of protein FeNO complexes
Selected oral communication - Nicholas Tucker (University of Strathclyde, UK)

Lipid oxidation

 

Chairs:
Paul Winyard (University of Exeter, UK)
Corinne Spickett (Aston University, Birmingham, UK)
Monday 18 April 2011
15:20 - 16:00
Analysis of oxidized and chlorinated lipids by mass spectrometry and relevance to signalling
Corinne Spickett (Aston University, Birmingham, UK)
16:00 - 16:40
Lipidomics of oxygenated bioactive metabolites of fatty acids
Anna Nicolaou (University of Bradford, UK)
16:40 - 18:00 Poster session with drinks reception

19:00 Conference dinner

Oxidative post-translational modifications

 

Chairs:
Corinne Spickett (Aston University, Birmingham, UK)
Philip Eaton (Kings College London, UK)
Tuesday 19 April 2011
09:00 - 09:40
Redox signalling via the cellular thiolstat - old suspects and new insights
Claus Jacob (Saarland University, Germany)
09:40 - 10:20
Protein redox sensors in the heart: From detection to functional consequences
Philip Eaton (Kings College London, UK)
10:20 - 11:00
Free radicals and redox signalling in T cells during inflammation and ageing
Helen Griffiths (University of Aston, Birmingham, UK)
11:00 - 11:30 Coffee/tea break

Oxidative post-translational modifications II

 

Chairs:
Michael Davies (Heart Research Institute, Sydney, Australia)
Andy Pitt (Aston University, Birmingham, UK)
Tuesday 19 April 2011
11:30 - 12:10
Protein thiol oxidoreduction in immunity
Pietro Ghezzi (Brighton & Sussex Medical School, UK)
12:10 - 12:30
Dexamethasone increases ROS production and T-cell suppressive capacity by anti-inflammatory macrophages
Selected oral communication - Marina Kraaij (Leiden University Medical Center, The Netherlands)
12:30 - 14:00 Lunch (with poster session)

14:00 - 14:40
Extracellular matrix oxidation and its consequences
Michael Davies (Heart Research Institute, Australia)
14:40 - 15:00
An integrated intracellular optical and extracellular electrochemical sensing platform for the study of reactive oxygen species (ROS) based cellular interactions
Selected oral communication - Philip Manning (Newcastle University, UK)
15:00 - 15:20
Oxidative DNA-damage during aging in a p47phox knockout model
Selected oral communication - Rajaraman Gnana Oli (University of Wuerzburg, Germany)
15:20 - 16:00
Cysteine residues function as catalysts for covalent protein modification: a role for thiyl radicals
Christian Schöneich (University of Kansas, USA)
16:00 Meeting close and departure