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10th Nitrogen Cycle Meeting 2004

2—4 September 2004

University of East Anglia, Norwich, UK



Organizers:
Clive Butler (Newcastle upon Tyne, UK)
David Richardson (East Anglia, UK)
Sponsor:
COST Office, ESF

10th Nitrogen Cycle Meeting 2004

Thursday 2 September 2004
16:30 - 16:45
Welcome and Introduction
Dave Richardson (East Anglia, UK)
16:45 - 17:10
Genomic potential of the anammox bacterium Kuenenia stuttgartiensis
Mike Jetten (Nijmegen, The Netherlands)
17:10 - 17:30
Application of anammox bacteria
Selected Oral Communication - Boran Kartal (Nijmegen, The Netherlands)
17:30 - 17:55
An unusual protein transport system involved in the biogenesis of N-cycle enzymes
Ben Berks (Oxford, UK)
17:55 - 18:15
Biochemical analysis of TorD: a chaperone involved in protein targeting and cofactor loading
Selected Oral Communication - Kostas Hatzixanthis (East Anglia, UK)
18:15 Wine Reception

The enzymology and biogenesis of the N-cycle

Chair:
Maria Delgado (Granada, Spain)
Friday 3 September 2004
09:00 - 09:25
Membrane-bound denitrification in the gram-positive bacterium, Bacillus azotoformans
Simon de Vries (Delft, The Netherlands)
09:25 - 09:45
Micro-aerobic denitrification in Neisseria meningitidis
Selected Oral Communication- Jonathan Rock (York, UK)
09:45 - 10:05
Nitrate reductases from Paracoccus denitrificans and Enterobacter cloacae; different activities towards nitrate and selenate
Selected Oral Communication - Carys Watts (Newcastle, UK)
10:05 - 10:25
Physico-chemical studies of cytochrome c nitrite reductase
Selected Oral Communication - Benidicte Burlat (East Anglia, UK)
10:25 - 11:00 Coffee/tea break

11:00 - 11:25
The complete denitrification pathway of the symbiotic, nitrogen fixing bacterium Bradyrhizobium japonicum
Eulogio Bedmar (Estacion Experimental del Zaidin, Granada, Spain)
11:25 - 11:45
Biogenesis of c-type cytochromes: the harder you look the more complex it becomes
Selected Oral Communication - James Allen (Oxford, UK)
11:45 - 12:05
A novel method of variant cytohrome cd1 expression
Selected Oral Communication - Richard Zajicek (Oxford, UK)
12:05 - 12:25
Reduced and oxidised structures of ResA reveal a redox-driven protein substrate selection mechanism in cytochrome c biosynthesis
Selected Oral Communication - Allister Crow (East Anglia, UK)
12:25 - 14:00 Lunch and poster session

Nitrogen and molybdenum uptake and assimilation

Chair:
Herman Bothe (Koeln, Germany)
Friday 3 September 2004
12:25 - 14:00 Lunch and poster session

14:00 - 14:25
Nitrogen fixation: key regulatory mechanisms
Ray Dixon (John Innes Centre, UK)
14:25 - 14:45
Using Artemis to root around in the genome sequence of Rhizobium leguminosarum
Selected Oral Communication - Lisa Crossman (Sanger Centre, Hinxton, UK)
14:45 - 15:05
Nitrate and nitrite transport in Esherichia coli
Selected Oral Communication - Wenjing Jia (Birmingham, UK)
15:05 - 15:25
Characterization of the nirK gene of Rhizobium etli CFN42
Selected Oral Communication - Emilio Bueno (Estacion Experimental del Zaidin, Granada, Spain)
15:25 - 16:00 Coffee/tea break

16:00 - 16:25
Nitrogen assimilation and nitrogen control in cyanobacteria
Enrique Flores (Instituto de Bioquimica Vegetal y Fotosintesis, Seville, Spain)
16:25 - 16:45
Alkaline cyanide biodegradation by Pseudomonas pseudoalcaligenes CECT5344
Selected Oral Communication - Victor M Luque-Almagro (Cordoba, Spain)
16:45 - 17:05
Molybdate transport in Bradyrhizobium japonicum
Selected Oral Communication - Chouhra Talbi (Estacion Experimental del Zaidin, Granade, Spain)
17:05 - 17:25
Ammonium sensing in Escherichia coli
Selected Oral Communication - Arnaud Javelle (John Innes Centre, Norwich, UK)

Reactive nitrogen species - sensing and metabolism

Chair:
Clive Butler (Newcastle, UK)
Saturday 4 September 2004
09:00 - 09:25
Nitric oxide and nitrosative stress tolerance in bacteria
Robert Poole (Sheffield, UK)
09:25 - 09:45
DNA binding properties of the Escherichia coli nitric oxide sensor NorR: towards an understanding of the regulation of flavorubredoxin
Selected Oral Communication - Nicholas Tucker (John Innes Centre, Norwich, UK)
09:45 - 10:05
N-oxides sensing in pseudomonas aeriginosa: expression and characterization of DNR, a FNR-crp type transcriptional regulator
Selected Oral Communication - Francesca Cutruzzola (Rome, Italy)
10:05 - 10:25
Purification and characterisation of cytochrome c' from Neisseria meningitidis
Selected Oral Communication - Wilhelmina Huston (York, UK)
10:25 - 11:00 Coffee/tea break

11:00 - 11:20
Roles of nitrate and nitrite reductases in Campylobacter jejuni
Selected Oral Communication - Marc Pittman (Sheffield, UK)
11:20 - 11:40
Transcriptional regulation of nitric oxide reduction in Ralstonia eutropha
Selected Oral Communication - Rainer Cramm (Berlin, Germany)
11:40 - 12:00
Transcriptional regulation and physiological studies of a hybrid cluster protein
Selected Oral Communication - Nina Filenko (Birmingham, UK)
12:00 - 12:20
Nitric oxide detoxification in almonella enterica serovar Typhimurium
Selected Oral Communication - Paul Mills (East Anglia, UK)
12:20 - 14:00 Lunch and poster session

Denitrification, nitrification and ammonification in symbionts, actinomycetes and the environment

Chair:
David Richardson (East Anglia, UK)
Saturday 4 September 2004
12:20 - 14:00 Lunch and poster session

14:00 - 14:25
Tracking nitrate reductase and denitrification in the environment
Laurent Phillipot (Bourgogne, France)
14:25 - 14:45
Biogeochemistry and microbial ecology of metal-sulfur-nitrogen interactions in wetlands
Selected Oral Communication - Suzanne Haaijer (Nijmegen, The Netherlands)
14:45 - 15:05
Phylogenetic markers for the assessment of ammonium oxidizing bacteria: hao/hzo versus amoA and 16S rRNA
Selected Oral Communication - Markus Schmid (Nijmegen, The Netherlands)
15:05 - 15:25
Proteome analysis of the anammox bacterium Kuenenia stuttgartiensis
Selected Oral Communication - Irina Cirpus (Nijmegen, The Netherlands)
15:25 - 16:00 Coffee/tea break

16:00 - 16:20
The active site structure of ba3-oxidase from Thermus thermophilus in the reaction with Nitric Oxide: Implications for Nitric Oxide reductase activity
Selected Oral Communication - Costas Varotsis (Crete, Greece)
16:20 - 16:40
Anammox in the past oceanic nitrogen cycle
Selected Oral Communication - Jack van de Vossenberg (Nijmegen, The Netherlands)
16:40 - 17:05
Nitrate Reduction in Streptomyces coelicolor
Selected Oral Communication - Gary Sawers (John Innes Centre, Norwich, UK)
17:05 - 17:20
Closing Lecture and Remarks - Dave Richardson (East Anglia, UK)