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Localization and activation of Ras-like GTPases

21—23 March 2005

Royal Agricultural College, Cirencester, UK



Organizers:
Anne Ridley (Ludwig Institute of Cancer Research, London, UK)
Miguel Seabra (Imperial College London, UK)
Monday 21 March 2005
13:30 - 15:00 Registration and coffee/tea

15:00 - 15:30
Arf-like GTPases and the organisation of membrane traffic
Sean Munro (MRC-LMB, Cambridge, UK)
15:30 - 16:00
Ras plasma membrane signalling platforms
John Hancock (Queensland, Australia)
16:00 - 16:15
A de-reacylation cycle regulates the compartmentalised localisation and activity of palmitoylated Ras isoforms
Oliver Rocks - Selected oral communication (EMBL, Heidelberg, Germany)
16:15 - 16:30
SAND-1 is an effector of RAB-7 and is required for endocytosis in Caenorhabditis elegans
Dmitry Poteryaev - Selected oral communication (Max Planck Gesellschaft, Tuebingen, Germany)
16:30 - 17:00
Protein semi-synthesis and its application to studies of structure and function of Rab GTPases
Kirill Alexandrov (Max Planck Institute of Molecular Physiology, Dortmund, Germany)
17:00 - 18:30 Welcome drinks reception

18:30 - 20:00 Dinner

20:00 - 20:30
Caveolin-1 mediates integrin-regulated membrane domain internalization
Miguel Del Pozo (CNIC, Madrid, Spain)
20:30 - 21:00
Insights into biological function across species: examining the role of Rab proteins in Yip1p function
Ruth Collins (Cornell, NY, USA)
Tuesday 22 March 2005
09:00 - 09:30
Membrane curvature and the control of GTP hydrolysis in Arf1 during COPI vesicle formation
Bruno Antonny (CNRS, Valbonne, France)
09:30 - 10:00
Palmitoylation and the subcellular localization of Ras proteins
Robert Deschenes (Medical College of Wisconsin, WI, USA)
10:00 - 10:15
Uncoupling of inhibitory and shuttling functions of rhoGDIs
Estelle Dransart - Selected oral communication (CNRS, Gif-sur-Yvette, France)
10:15 - 10:45 Coffee/tea break

10:45 - 11:15
A role for effectors in Rab localization
Suzanne Pfeffer (Stanford, CA, USA)
11:15 - 11:45
Mechanism and role of localized activation of Rho-family GTPases in growth factor-stimulated fibroblasts and neuronal cells
Michiyuki Matsuda (Osaka, Japan)
11:45 - 12:00
How ArfGEFs couple stimulation of GDP/GTP exchange to membrane recruitment of Arf proteins
Jacqueline Cherfils - Selected oral communication (CNRS, Gif-sur-Yvette, France)
12:00 - 13:30 Lunch

13:30 - 16:00 Formal poster session and coffee/tea

16:00 - 16:30
Localization and function of Arf family GTPases
Julie Donaldson (National Heart, Lung and Blood Institute, MD, USA)
16:30 - 16:45
ARL1 and ARF6 have essential roles in Trypanosoma brucei
Helen Price- Selected oral communication (York, UK)
16:45 - 17:15
Ran and assembly of the nuclear envelope
Wolfram Antonin (EMBL, Heidelberg, Germany)
17:15 - 17:30
Design of a FRET-based biosensor to visualize the spatio-temporal activation of Pak1 kinase in living cells
Maria Carla Parrini - Selected oral communication (INSERM, Paris, France)
18:30 - 23:00 Meeting dinner and social

Wednesday 23 March 2005
09:00 - 09:30
Regulation of RhoE localization and function
Anne Ridley (Ludwig Institute for Cancer Research, London, UK)
09:30 - 10:00
Rab targeting signals and mechanisms
Miguel Seabra (Imperial College London, UK)
10:00 - 10:15
A novel mode of GEF-GTPase interaction drives Rac1 targeting d activation by beta-Pix
Jean Paul ten Klooster - Selected oral communication (Sanquin Research, Amsterdam, Netherlands)
10:15 - 10:45 Coffee/tea break

10:45 - 11:15
A farnesyl-electrostatic switch regulates K-Ras association with mitochondria and stimulates apoptosis
Mark Philips (New York, USA)
11:15 - 11:45
A novel Golgi-localized Cdc42-GAP that functions downstream of ARF1 to control Arp2/3 complex/F-actin dynamics
Philippe Chavrier (Institut Curie, Paris, France)
11:45 - 12:00
Activation of endogenous Ras is predominant at the plasma membrane
Ignacio Rubio - Selected oral communication (Friedrich Schiller, Jena, Germany)
12:00 - 13:30 Lunch