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Compartmentalization of cyclic AMP signalling

29—30 March 2006

King's College, Cambridge, UK



Organizers:
Dermot Cooper (University of Cambridge, UK)
Miles Houslay (University of Glasgow, UK)
Manuela Zaccolo (University of Padova, Italy)

Timetable

Wednesday 29 March 2006Thursday 30 March 2006
10:40 - 12:30
Session One

14:00 - 16:55
Session Two

09:10 - 12:05
Session Three

13:30 - 15:20
Session Four


Session One

Chair:
Miles Houslay (University of Glasgow, UK )
Wednesday 29 March 2006
09:00 - 10:30 Registration and Coffee

10:30 - 10:40 Welcome and Introduction

10:40 - 11:15
Molecular architecture of signal transduction complexes
John Scott (Oregon Health Sciences University)
11:15 - 11:50
Use of single-cell imaging techniques to assess the regulation of cAMP dynamics
Debbie Willoughby (University of Cambridge, UK)
11:50 - 12:10
The high-affinity AKAP 18delta-PK interaction yields novel PKA anchoring disrupter peptides
Selected oral communication - Enno Klussman (Freie Universität Berlin, Germany)
12:10 - 12:30
Phosphodiesterase-4 influences the PKA Phorphorylation status and membrane translocation of G-protein receptor kinase 2 (GRK2) in HEK293b2 cells and cardiac myocytes
Selected oral communication - George Baillie (University of Glasgow, UK)
12:30 - 14:00 Lunch and Poster viewing

Session Two

Chair:
Manuela Zaccolo (University of Padova, Italy)
Wednesday 29 March 2006
14:00 - 14:35
The molecular machinery for cAMP-dependent immunomodulation in T cells
Kjetil Tasken (University of Oslo, Norway)
14:35 - 15:10
Epac and Rap1 in the control of cell adhesion
Johannes Bos (Utrecht University, The Netherlands)
15:10 - 15:30
cAMP-mediated gene expression in human thyroid cells
Selected oral communication - Wilma Van Stavern (Free University of Brussels )
15:30 - 16:00 Coffee/Tea break

16:00 - 16:35
Layers of organization of cAMP microdomains
Dermot Cooper (University of Cambridge, UK)
16:35 - 16:55
Adenylyl cyclase type 8 distinguishes between capacitative and OAG-activated Ca2+ entry in HEK 293 cells
Selected oral communication - Agnes Martin (University of Cambridge, UK)
16:55 - 18:00 Free time

18:00 - 19:30 Poster session and drinks reception

19:30 - 22:00 Meeting dinner

Session Three

Chair:
Dermot Cooper (University of Cambridge, UK)
Thursday 30 March 2006
09:00 - 09:10 Introduction

09:10 - 09:45
Grégoire Vandecasteele (INSERM, France)
09:45 - 10:05
AKAP-complex regulates the Ca2+ reuptake into heart sarcoplasmic reticulum
Selected oral communication - Birgitte Lygren (University of Oslo, Norway)
10:05 - 10:20
Soluble Adenylyl Cyclase Reveals the Significance of Compartmentalized cAMP on Pulmonary Microvascular Endothelial Cell (PMVEC) Barrier
Selected oral communication - Sarah Sayner (University of South Alabama)
10:20 - 10:50 Coffee/Tea

10:50 - 11:25
Imaging the cAMP-dependent transduction pathway
Manuela Zaccolo (University of Padova, Italy)
11:25 - 11:45
cAMP oscillations restrict spatial redistribution of protein kinase A in insulin-secreting cells
Selected oral communication - Anders Tengholm (Uppsala University, Sweden)
11:45 - 12:05
Identification of the macromolecular comlex responsible for P13Kgamma dependent regulation of cAMP levels
Selected oral communication - Emilio Hirsch (University of Torino, Italy)
12:05 - 13:30 Lunch and Poster Viewing

Session Four

Chair:
Kjetil Tasken (University of Oslo, Norway )
Thursday 30 March 2006
13:30 - 14:05
RACK1 and beta-arrestin compete to sequester cAMP phosphodiesterase-4D5 (PDE4D5)
Miles Houslay (University of Glasgow, UK)
14:05 - 14:25
Dynamic regulation of (beta)-adrenergic signalling by compartmentalised PDE2
Selected oral communcation - Marco Mongillo (University of Padua, Italy)
14:25 - 15:00
cGMP signalling in epithelial transport
Jonathan Day (University of Glasgow, UK )
15:00 - 15:20
Receptors that stimulate cAMP formation sensitize IP3 receptors
Selected oral communication - Skarlatos Dedos (University of Cambridge)
15:20 - 16:15 Coffee/tea and meeting close