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Third intracellular proteolysis meeting. Joint meeting of the INPROTEOLYS network and the Biochemical Society

5—7 March 2008

Auditorio de Tenerife, Santa Cruz de Tenerife, Canary Islands, Spain



Organizers:
Manuel S. Rodríguez (CICbioGUNE, Spain)
Gemma Marfany (University of Barcelona, Spain)
Dimitris Xirodimas (University of Dundee, UK)
Eduardo Salido (ITB, University of La Laguna, Spain)
Rosa Farràs (Centro de Investigación Príncipe Felipe, Spain)

Timetable

Wednesday 5 March 2008Thursday 6 March 2008Friday 7 March 2008
08:00 - 11:00
Session 1: Lysosomal pathway

11:30 - 13:00
Session 2: Ubiquitin and cell membrane associated degradation

14:30 - 19:30
Session 3: Mechanisms of specificity of the ubiquitin proteasome pathway part 1

09:00 - 12:45
Session 3: Mechanisms of specificity of the ubiquitin proteasome pathway part 2

14:30 - 19:30
Session 4: Regulation of transcription through the ubiquitin-proteasome pathway

09:00 - 12:30
Session 5: Ubiquitin-like molecules

14:30 - 19:30
Session 6: Regulation of the proteasome activity




Session 1: Lysosomal pathway

Chair:
Patricia Boya (CSIC, Spain)
Wednesday 5 March 2008
08:00 - 09:00
Registration
09:00 - 09:30
Welcome
09:30 - 10:05
Lysosomal control of tumor cell survival
Marja Jäättelá  (Institute for Cancer Biology, Denmark)
10:05 - 10:40
Self-eating or self-killing: the essential dilemma of a stressed cancer cell
Guido Kroemer (CNRS, France)
10:40 - 11:00
The autophagic machinery is necessary for removal of cell corpses from the developing retinal neuroepithelium
Patricia Boya (Centro de Investigaciones Biológicas, CSIC, Spain)
11:00 - 11:30 Coffee/tea break

Session 2: Ubiquitin and cell membrane associated degradation

Chair:
Eduardo Salido (ITB, University of La Laguna, Spain )
Wednesday 5 March 2008
11:30 - 12:05
Versatile role of ubiquitin in the trafficking of yeast transporters
Naima Belgareh (Institute Jacques-Monod, France)
12:05 - 12:40
Protein quality control and degradation at the endoplasmic reticulum
Thomas Sommer (Max-Delbruck-Center for Molecular Medicine, Germany)
12:40 - 13:00
The role of a novel p97/VCP-interacting motif (VIM) in ER-associated degradation
Petek Ballar (University of Maryland Biotechnology Institute, USA )

Session 3: Mechanisms of specificity of the ubiquitin proteasome pathway part 1

Chair:
Dimitris Xirodimas (University of Dundee, UK )
Wednesday 5 March 2008
14:30 - 15:05
Targeting ubiquitin networks
Ivan Dikic (Goethe University, Germany)
15:05 - 15:25
E3-independent self-ubiquitination of Ub-binding proteins
Daniela Hoeller (Biocenter Innsbruck, Austria)
15:25 - 15:45
Development of TUBEs (Tandem Ubiquitin Binding Entities) to study ubiquitin dependent processes
Roland Hjerpe (CICbioGUNE, Spain)
15:45 - 16:15 Coffee/tea break

16:15 - 16:40
CHIP mediated degradation and DNA damage dependent stabilization regulates base excision repair proteins
Grigory L. Dianov (University of Oxford, UK)
16:40 - 17:00
In vivo biotinylation of neuronal ubiquitin substrates
Ugo Mayor (Wellcome Trust Cancer Research UK Gurdon Institute, Cambridge, UK )
17:00 - 18:00
Round table discussion
18:00 - 19:30
Sunset poster session 1 and Welcome Drinks Reception

Session 3: Mechanisms of specificity of the ubiquitin proteasome pathway part 2

Chair:
Christine Blattner (Institute of Toxicology and Genetics, Germany)
Thursday 6 March 2008
09:00 - 09:35
The ubiquitin-proteasome system in Epstein-Barr virus infected cells
Maria Masucci (Karolinska Institute, Sweden)
09:35 - 10:10
Identification of novel inhibitors of deubiquitinating enzymes
Seth Goldenberg (Progenra Inc, USA)
10:10 - 10:30
Functional characterization of several structural domains of the deubiquinating enzyme USP25
Amanda Denuc (University of Barcelona, Spain)
10:30 - 11:00 Coffee/tea

11:00 - 11:35
Regulation of separase: Securin-independent modes of regulating the giant protease that triggers anaphase
Olaf Stemmann (Max-Planck-Institute of Biochemistry, Germany)
11:35 - 12:10
Aggresome formation in response to Ala-Ala-Phe-chloromethylketone is independent of the inhibition of tripeptidyl peptidase II
Luis C. Antón (CSIC, Spain)
12:10 - 12:45
Are F-box proteins with a C-terminal domain homologous to the tobacco lectin involved in protein degradation in plants?
Els Van Damme (Ghent University, Belgium)

Session 4: Regulation of transcription through the ubiquitin-proteasome pathway

Chair:
Marc Piechazyk (CNRS, France)
Thursday 6 March 2008
14:30 - 15:05
The ubiquitin ligase E6-AP and its role in human disease
Martin Scheffner (University of Konstanz, Germany)
15:05 - 15:25
SAMe-deficient tumor hepatocyte cell line (SAM-D) shows wild type and active p53 together with UV resistance
Nuria Martinez (CICbioGUNE, Spain.)
15:25 - 15:45
Role of ubiquitin and SUMO molecules in the regulation of the inhibitory function of IkBa on NFkB activity
Valerie Lang (CICbioGUNE, Spain)
15:45 - 16:15 Coffee/tea break

16:15 - 16:40
Coordinated deregulations of Fos-related antigen 1 and -2 proteasomal degradations in cancer cells
Marc Piechaczyk (CNRS, France )
16:40 - 17:00
JunB breakdown in G2 is required for proper mitosis
Rosa Farràs (Centro de Investigación Príncipe Felipe, Spain)
17:00 - 18:00
Round table discussion
18:00 - 19:30
Sunset poster session 2

Session 5: Ubiquitin-like molecules

Chair:
Gemma Marfany (University of Barcelona, Spain)
Friday 7 March 2008
09:00 - 09:35
Role of poly-SUMO chains in targeting proteins for degradation
Ronald Hay (University of Dundee, UK)
09:35 - 10:10
The role of Sumoylation during Drosophila metamorphosis
Ana Talamillo (CICbioGUNE, Spain)
10:10 - 10:30
Drosophila transcriptional adaptor Ada3 as a target for SUMOylation in vivo
Benjamin Grau (CSIC, Spain)
10:30 - 11:00 Coffee/tea break

11:00 - 11:35
Regulation of SUMOylation by reversible oxidation of SUMO conjugating enzymes
Guillaume Bossis (CNRS, France)
11:35 - 11:55
SUMO-binding domains are involved in the regulation of tumour suppressors by the Kaposi´s sarcoma-associated herpesvirus latent protein LANA2
Carmen Rivas (CSIC, Spain)
11:55 - 12:30
Ribosomal proteins; a novel target for the NEDD8 pathway
Dimitris Xirodimas (University of Dundee, UK)

Session 6: Regulation of the proteasome activity

Chair:
Olivier Coux (CNRS, France)
Friday 7 March 2008
14:30 - 15:05
A Mason's approach to the 19S regulatory particle of the proteasome
Michael Glickman (The Technion, Israel)
15:05 - 15:25
The ubiquitin ligase Mdm2 interacts with the 19S regulatory subunit of the proteasome
Roman Kulikov (Institute of Toxicology and Genetics, Germany)
15:25 - 15:45
Scaled-down purification of 20S proteasome from human tissue sample: application on normal and tumor colorectal cells
Sandrine Uttenweiler-Joseph (CNRS, France)
15:45 - 16:15 Coffee/tea break

16:15 - 16:40
Diversity of proteasomal missions: fine tuning of the immune response
Michael Groll (Munich Technical University, Germany)
16:40 - 17:00
Remodeling of the ubiquitin proteasome system in response to Interferons
Elke Krüger (Institute of Biochemistry, Germany)
17:00 - 18:00
Round table discussion and Conclusion
18:00 - 19:30
Sunset poster session 3
21:00 - 23:00 Gala dinner