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Biochemical Society / Wellcome Trust Focused Meeting

28—29 October 2008

Hinxton Hall, Cambridge, UK



Organizers:
Devin Leake (Thermo Fisher Scientific, USA)
Simon Baker (Oxford Brookes University, UK)
Ian Kavanagh (Thermo Fisher Scientific, UK)
Jeremy Gillespie (Thermo Fisher Scientific, UK)
Simon Hughes (British Library, UK)

Nucleic acid detection technologies

Chair:
Simon Baker (Oxford Brookes University, UK)
Tuesday 28 October 2008
08:30 - 09:50 Registration

09:50 - 10:00
Introduction
Simon Baker (Oxford Brookes University, UK)
10:00 - 10:40
Analysing gene expression levels in single cells
Ed Southern (Oxford Gene Technology Ltd, UK)
10:40 - 11:10
QPCR and the state of the art
Ian Kavanagh (Thermo Fisher Scientific)
11:10 - 11:30 Tea/coffee break

11:30 - 12:10
Coupling stable isotopes and nucleic acid detection to resolve microbial functions
Andrew Whiteley (NERC CEH Oxford, UK)
12:10 - 12:40
Nucleic acid detection and quantification in the developing world
Jim Huggett (UCL, UK)
12:40 - 14:00 Lunch and poster session

14:00 - 14:20
Single-nucleotide primer extension (SNuPE) method for the multiplex detection of various DNA sequences ¿ from detection of point mutation to microbial ecology
Selected oral communication - Marcell Nikolausz (Helmholtz Centre for Environmental Research, Germany)

Single cell molecular biology

Tuesday 28 October 2008
14:20 - 14:40
The SAFE project, towards non-invasive prenatal diagnosis
Selected oral communication - Debbie Maddocks (University of the West of England, UK)
14:40 - 15:20
Single cell genetic analysis for preimplantation genetic diagnosis of inherited disease
Alan Handyside (The Bridge Centre, UK)
15:20 - 16:00 Tea/coffee break

16:00 - 16:40
Miniaturized PCR systems for cancer diagnosis
Philip Day (University of Manchester, UK)
16:40 - 17:00
Oxygen-DNA Interactions: spectrophotometric quantification of nucleic acids in solution is altered by the level of dissolved oxygen
Selected oral communication - Rupak Doshi (University of Manchester, UK)
17:00 - 19:00 Wine reception and poster viewing

19:00 - 21:00 Meeting Dinner

Applications of detection technology

Chairs:
Devin Leake (ThermoFisher Scientific)
Ian Kavanagh (ThermoFisher Scientific)
Wednesday 29 October 2008
09:00 - 09:40
Real-time cold-PCR: a new platform for highly improved mutation detection in cancer and genetic testing
Mike Makrigiorgos (Harvard Medical School, USA)
09:40 - 10:20
Mutation scanning using high-resolution amplicon melting
Claire Taylor (Cancer Research, UK)
10:20 - 10:40
A paradox of multiplex DNA melting on the surfaces
Selected oral communication - Alexander Chagovetz (University of Utah, USA)
10:40 - 11:10 Tea/coffee break

11:10 - 11:50
The Polymerase Chain Reaction (PCR) in forensic genetics
Niels Morling (University of Copenhagen, Denmark)
11:50 - 12:30
Method for extraction, reverse transcription and PCR applicable to single cell analysis
Linda Strömbom (TATAA Biocenter AB, Sweden)
12:30 - 13:30 Lunch and poster viewing

13:30 - 14:10
Diagnostic validation of conformation sensitive capillary electrophoresis
Chris Mattocks (National Genetics Reference Library, UK)
14:10 - 14:50
Functionalized nanoparticles for nucleic acid sequence analysis using optical spectroscopies
Duncan Graham (University of Strathclyde, UK)
14:50 - 15:30
Optical fibre-based detection of DNA hybridization
Anna Hine (Aston University, UK)
15:30 - 16:00 Tea/coffee break with poster viewing

16:00 - 16:40
Multiple displacement amplification (MDA) of genomic DNA: detection and genomics from samples with low DNA content and single cell sequencing
Roger Lasken (J Craig Venter Institute, USA)
16:40 - 16:45 Award of Poster Prize
Sponsored by The British Library