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Quantitative Proteomics and Data Analysis

24—25 March 2014

Queen Hotel, Chester, UK



Biochemical Society Training Day

 

This training day will meet two needs:

 

1.  It will continue the format of the previous two training days, and will use a mixture of lectures and small group teaching to achieve a high level of awareness of the issues and problems inherent in quantitative proteomics.

 

2.  It will precede the third iteration of a highly successful meeting on Quantitative Proteomics (ProteoMMX 3.0). This ensures that newcomers to the field are steeped in the jargon, and indeed, the complexity, of quantitative proteomics before they take part in a highly detailed but relaxed and friendly conference on this specific topic.

 

Organizers:
Rob Beynon (University of Liverpoool, United Kingdom)
Kathryn Lilley (Cambridge University, United Kingdom)
Claire Eyers (University of Liverpool, United Kingdom)
Andy Jones (University of Liverpool, United Kingdom)
Simon Hubbard (University of Manchester, United Kingdom)
Victoria Harman (University of Liverpool, United Kingdom)
Sponsors:
Monday 24 March 2014
12:30 - 13:45 Registration and lunch

13:45 - 14:00 Introduction and Welcome
Rob Beynon (University of Liverpool, United Kingdom)

Session 1: Landscapes and strategies

 

Monday 24 March 2014
14:00 - 14:30
An overview of quantitative proteomics workflows – experimental design, workflows and analysis
Rob Beynon (University of Liverpool, United Kingdom)
14:30 - 15:00
Considerations in experimental design and statistical analysis in quantitative proteomics
Kathryn Lilley (University of Cambridge, United Kingdom)
15:00 - 15:30
Label-free or label mediated, relative or absolute?
Claire Eyers (University of Liverpool, United Kingdom)
15:30 - 16:00 Tea and Coffee break

16:00 - 16:30
Bioinformatics for quantitative proteomics
Simon Hubbard (University of Manchester, United Kingdom)
16:30 - 17:00
Tools and standards in reporting quantitative proteomics data
Andy Jones (University of Liverpool, United Kingdom)
17:00 - 17:30 Q & A session


Tuesday 25 March 2014
09:00 - 12:00

Session 2 – ‘Pick and mix’ round tables

Three rounds of 1 hour each. All participants attend three of the tables listed below, based on optimisation of their priorities. Coffee/Tea available throughout as required (from about 10).

Tuesday 25 March 2014
09:00 - 12:00
Table A Quantification using MASCOT/Distiller
John Cottrell (Matrix Science)
09:00 - 12:00
Table B Designing an SRM quantification study
Stephen Holman and Philip Brownridge (Universities of Liverpool and Manchester)
09:00 - 12:00
Table C Quantification using MaxQuant
Sara ten Have (University of Dundee, United Kingdom)
09:00 - 12:00
Table D Inspection, visualisation and analysis of quantitative proteomics data
Hans Vissers (Waters Corp)
09:00 - 12:00
Table E Statistical design of a quantification experiment
Kathryn Lilley (University of Cambridge, United Kingdom)
09:00 - 12:00
Table F Intensity-based label free analysis using Progenesis
Martin Wells (Nonlinear Dynamics)
09:00 - 12:00
Table G Data independent quantification and SWATH-type approaches
Cathy Lane (AB SCIEX)
09:00 - 12:00
Table H SILAC and whole organism studies
Dean Hammond (University of Liverpool, United Kingdom)
09:00 - 12:00
Table I Tools for label-free quantification using PEAKS 7
Paul Shan (Bioinformatics Solutions Inc)
09:00 - 12:00
Table J Absolute and relative quantification
Stefan Tenzer (University of Mainz, Germany)