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54th Harden Conference - Enzymology: Emerging Trends and Future Prospects

20—24 August 2002

St Martin's College, Ambleside, Lake District, UK



Timetable

Wednesday 21 August 2002Thursday 22 August 2002Friday 23 August 2002
08:30 - 22:00
Structural Approaches

08:30 - 18:30
Theory, Computation and Mechanism

08:30 - 22:00
Protein Engineering, Enzyme Evolution and Synthetic Models


Structural Approaches

Organizers:
Dr Alan Berry (Leeds, UK)
Professor Nigel Scrutton (Leicester, UK)
Professor Steve Chapman (Edinburgh, UK)
Sponsors:
AstraZeneca
BMG Labtechnologies
Wednesday 21 August 2002
08:30 - 09:10
Molecular basis of proton motive force generation; structure of escherichia coli formate dehydrogenase-N
So Iwata (Imperial College London, UK)
09:10 - 09:50
Crystallographic studies of redox enzymes
David Leys (Leicester, UK)
09:50 - 10:30
Characterising enzymes - what's the question? NMR may provide the answer
Geoff Moore (East Anglia, UK)
10:30 - 11:00 Tea/ Coffee Break

11:00 - 11:40
EPR and MCD studies of metalloenzymes
Myles Cheesman (East Anglia, UK)
11:40 - 12:20
Single molecule fluorescence studies of acto-myosin
Clive Bagshaw (Leicester, UK)
12:20 - 19:00 Lunch and Free Time

19:00 - 20:00 Dinner

20:00 - 21:00
The pyruvate dehydrogenase complex: a paradigm for multi-step catalysis by a molecular machine
Richard Perham (Cambridge, UK)
21:00 - 22:00
Structural enzymology of quinoproteins
Simon Philips (Leeds, UK)

Theory, Computation and Mechanism

Thursday 22 August 2002
08:30 - 09:10
Modelling enzyme reactions
Adrian Mulholland (Bristol, UK)
09:10 - 09:50
Computational studies of hydrogen tunnelling in enzymes
Mike Sutcliffe (Leicester, UK)
09:50 - 10:30
H-tunnelling and dynamics effect in enzyme catalyzed hydride transfer
Amnon Kohen (Iowa, USA)
10:30 - 11:00 Tea/ Coffee Break

11:00 - 11:40
The role of iron-oxygenases in eukaryotic dioxygen sensing
Chris Schofield (Oxford, UK)
11:40 - 12:20
Biochemical and structural analysis of the molybdenum cofactor biosythesis protein MobA
David Lawson (John Innes Centre, Norwich, UK)
12:20 - 13:20 Lunch

13:20 - 13:35
The reactions catalysed by biliverdin reductases (BVR-A and BVR-B) enzymes with complicated behaviour
Selected Poster Presentation - Tim Mantle (Dublin, Ireland)
13:35 - 13:50
Towards a mechanism for metallo-ß-lactamases
Selected Poster Presentation - James Spencer (Bristol, UK)
13:50 - 14:05
Metalloprotein and synchrotron radiation
Selected Poster Presentation - Loretta Mary Murphy (Daresbury Laboratory, Warrington, UK))
14:05 - 16:30 Poster and Poster Communication

16:30 - 17:30
Designed evolution of flavoprotein oxygenases and oxidases
Willem van Berkel (Wagenigen, Netherlands)
17:30 - 18:30
Structural studies of enzyme mechanism
Jim Naismith (St Andrews, UK)
19:00 - 23:00 Conference Dinner

Protein Engineering, Enzyme Evolution and Synthetic Models

Friday 23 August 2002
08:30 - 09:10
Rational modular design of artificial enzymes
Paul Barker (Cambridge, UK)
09:10 - 09:50
Protein engineering of cytochrome P450cam
Luet Wong (Oxford, UK)
09:50 - 10:30
Protein engineering of nitric oxide synthase
Simon Daff (Edinburgh, UK)
10:30 - 11:00 Tea/ Coffee Break

11:00 - 11:40
Dissecting an enzyme - model compounds for the galactose oxidase radical site
Malcolm Halcrow (Leeds, UK)
11:40 - 12:20
Directed evolution of aldolases for new function
Alan Berry (Leeds, UK)
12:20 - 18:00 Lunch and Free Time

18:30 - 19:30 Dinner

20:00 - 21:00
Singular structures and multiple functions in de novo designed haem protein maquettes
Les Dutton (Sponsored By The British Biophysical Society) (Philadelphia, PA, USA)
21:00 - 22:00
The structure and mechanism of family II inorganic pyrophosphatases: an open-and-shut case
Scott White (Birmingham, UK)