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Extraction, Quantification and Visualization of polyP

8 September 2015

MRC Laboratory for Molecular and Cellular Biology, UCL, London, UK



08:45 – 09:30  Registration

 

09:30 – 10:45  Session 1

- polyP extraction and purification procedures.

- polyP enzymology; the purification and use of. recombinant His-EcPPK, His-ScPPX1 and His-ScDDP1.

- Analysis of polyP by gel electrophoresis and visualization by Toulidine Blue and DAPI staining.

- Quantification of polyP by DAPI or JC-D8 fluorescence and Malachite green assay.

- Microscopy detection of polyp.

 

10:45 – 11:00  Tea/Coffee Break

 

11:00 – 12:15  Session 2

- polyP extraction and purification procedures.

- polyP enzymology; the purification and use of. recombinant His-EcPPK, His-ScPPX1 and His-ScDDP1.

- Analysis of polyP by gel electrophoresis and visualization by Toulidine Blue and DAPI staining.

- Quantification of polyP by DAPI or JC-D8 fluorescence and Malachite green assay.

- Microscopy detection of polyp.

 

12:15 – 13:15  Lunch

 

13:15 – 14:30  Session 3

- polyP extraction and purification procedures.

- polyP enzymology; the purification and use of. recombinant His-EcPPK, His-ScPPX1 and His-ScDDP1.

- Analysis of polyP by gel electrophoresis and visualization by Toulidine Blue and DAPI staining.

- Quantification of polyP by DAPI or JC-D8 fluorescence and Malachite green assay.

- Microscopy detection of polyp.

 

14:30 – 14:45  Tea/Coffee Break

 

14:45 – 16:00  Session 4

- polyP extraction and purification procedures.

- polyP enzymology; the purification and use of. recombinant His-EcPPK, His-ScPPX1 and His-ScDDP1.

- Analysis of polyP by gel electrophoresis and visualization by Toulidine Blue and DAPI staining.

- Quantification of polyP by DAPI or JC-D8 fluorescence and Malachite green assay.

- Microscopy detection of polyp.

 

16:00 – 16:15 Tea/Coffee Break

 

16:15 – 17:30 Session 5

- polyP extraction and purification procedures.

- polyP enzymology; the purification and use of. recombinant His-EcPPK, His-ScPPX1 and His-ScDDP1.

- Analysis of polyP by gel electrophoresis and visualization by Toulidine Blue and DAPI staining.

- Quantification of polyP by DAPI or JC-D8 fluorescence and Malachite green assay.

- Microscopy detection of polyp.

 

17:30Close/Depart