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65th Harden - Enzymes: Nature's molecular machines

17—21 August 2008

University of Cumbria, Ambleside, UK



Organizers:
Andrew Munro (Manchester Interdisciplinary Biocentre, UK)
Martin J. Warren (University of Kent, UK)
Sponsors:

Timetable

Sunday 17 August 2008Monday 18 August 2008Tuesday 19 August 2008Wednesday 20 August 2008Thursday 21 August 2008
19:30 - 21:10
Hot topics

09:00 - 12:50
Coenzymes and metabolic pathways

19:00 - 21:00
Emerging concepts

09:00 - 12:50
Redox enzymes

16:00 - 18:15
Novel enzymes and processes

09:00 - 12:50
Enzyme mechanism and chemical biology

19:30 - 20:50
Synthetic biology

09:00 - 12:50
Structural and biophysical methods


Hot topics

Chair:
Andrew Munro (Manchester Interdisciplinary Biocentre, UK)
Sunday 17 August 2008
16:00 - 18:00 Registration

18:00 - 19:30 Dinner

19:30 - 20:10
From Pasteur and Darwin to new enzyme activities
Alan Berry (University of Leeds, UK)
20:10 - 21:10
Stereochemical control on antibiotic-producing polyketide synthase multienzymes
Plenary Lecture - Peter Leadlay (University of Cambridge, UK)

Coenzymes and metabolic pathways

Chair:
Chris Abell (University of Cambridge, UK)
Monday 18 August 2008
09:00 - 09:40
Catalytic mechanisms of the catechol and carotenoid dioxygenases
Timothy Bugg (University of Warwick, UK)
09:40 - 10:00
Structures of acyl carrier protein synthase and ketoreductase: modules in type II polyketide synthesis, suggest a common structural motif for ACP recognition
Selected oral communication - Andrea Hadfield (University of Bristol, UK)
10:00 - 10:20
Structural determination of the 6,4-photolyase from Drosophila Melanogaster
Selected oral communication - Max Cryle (Max-Planck Institute for Medical Research, Heidelberg, Germany)
10:20 - 11:00
The riboflavin synthase family
Markus Fischer (University of Hamburg, Germany)
11:00 - 11:30 Coffee/tea break

11:30 - 12:10
Unusual molecular strategies of enzymes involved in heme and chlorophyll biosynthesis
Dieter Jahn (Technical University of Braunschweig, Germany)
12:10 - 12:50
The making of metabolic microcompartments in bacteria
Martin J. Warren (University of Kent, UK)
12:50 - 15:30 Lunch and free time

15:30 - 17:30 Poster session 1 (Posters P001-P027)

17:30 - 19:00 Dinner

Emerging concepts

Chair:
Nigel Scrutton (University of Manchester, UK)
Monday 18 August 2008
19:00 - 19:40
Proton/electron coupling in cytochrome oxidase: insights from IR spectroscopy
Peter Rich (University College London, UK)
19:40 - 20:00
Assessing the chemical versatility of catalytic residues and cofactors
Selected oral communication - Gemma Holliday (EMBL-EBI, Cambridge, UK)
20:00 - 20:20
High-resolution ultrasonic spectroscopy for real-time monitoring of enzyme kinetics and protein conformational transitions
Selected oral communication - Vitaly Buckin (University College Dublin, Ireland)
20:20 - 21:00
The modification and degradation of heme in biological systems
Emma Raven (University of Leicester, UK)

Redox enzymes

Chair:
Tadhg Begley (Cornell University, USA)
Tuesday 19 August 2008
09:00 - 09:40
Mechanisms that control catalysis: what tunes reactivity of the NO synthase enzyme-product complex?
Dennis Stuehr (Lerner Institute, Cleveland, USA)
09:40 - 10:00
Structure-function studies on berberine bridge enzyme
Selected oral communication - Andreas Winkler (Graz University of Technology, Austria)
10:00 - 10:20
The crystal structure of CYP125: P450 drug targeting and cholesterol metabolism in the pathogen Mycobacterium tuberculosis
Selected oral communication - Kirsty McLean (Manchester Interdisciplinary Biocentre, UK)
10:20 - 11:00
Experimental probes of hydrogen tunnelling in enzyme systems
Nigel Scrutton (University of Manchester, UK)
11:00 - 11:30 Coffee/tea break

11:30 - 12:10
Mechanistic studies of oxidative halophenol dehalogenation by Heme-containing peroxidases
John Dawson (University of South Carolina, USA)
12:10 - 12:50
Hemoproteins: catalysts and sensors
Paul Ortiz de Montellano (University of California, San Francisco, USA)
12:50 - 13:50 Lunch

13:50 - 16:00 Free Time and Boat trip on Lake Windermere

Novel enzymes and processes

Chair:
Emma Raven (University of Leicester, UK)
Tuesday 19 August 2008
16:00 - 16:40
Getting in a flap with DNA: how flap endonucleases achieve structure specific DNA cleavage
Jane Grasby (University of Sheffield, UK)
16:40 - 17:20
Structure and mechanism in cytochrome P450 redox systems
Andrew Munro (Manchester Interdisciplinary Biocentre, UK)
17:20 - 18:00
Thiamin biosynthesis - still yielding fascinating biological chemistry
Tadhg Begley (Cornell University, USA)
18:00 - 18:15
Advances in precision microvolume spectrophotometry and fluorescence spectroscopy
Bob Keighley (Varian Ltd, UK)
18:15 - 20:15 Conference Dinner

Enzyme mechanism and chemical biology

Chair:
Paul Ortiz de Montellano (University of California, San Francisco, USA)
Wednesday 20 August 2008
09:00 - 09:40
New approaches in enzymology
Chris Abell (University of Cambridge, UK)
09:40 - 10:00
Flavins: the winners and losers of B12 biosynthesis
Selected oral communication - Andrew Lawrence (University of Kent, UK)
10:00 - 10:20
How to interchange decarboxylase and oxidase activities with a single amino acid substitution
Selected oral communication - Stephen Bornemann (John Innes Centre, Norwich, UK)
10:20 - 11:00
Evolution of enzyme activity
Richard Pickersgill (Queen Mary, University of London, UK)
11:00 - 11:30 Coffee/tea break

11:30 - 12:10
Dynamics of domain movements and their role in force generation by myosin motors
Mike Geeves (University of Kent, UK)
12:10 - 12:50
How to BLUF ¿ insights into blue light signaling
Ilme Schlichting (Max Planck Institute, Heidelberg, Germany)
12:50 - 16:00 Lunch and free time

16:00 - 18:00 Poster session 2 (Posters P028-P055)

18:00 - 19:30 Dinner

Synthetic biology

Chair:
Richard Pickersgill (Queen Mary, University of London, UK)
Wednesday 20 August 2008
19:30 - 20:10
Dissecting structure and mechanism of enzyme inhibition - practical lessons from drug discovery
Alan Wallace (AstraZeneca, Charnwood, UK)
20:10 - 20:50
Sugar and proteins: strategies in synthetic biology
Ben Davis (University of Oxford, UK)

Structural and biophysical methods

Chair:
Martin Warren (University of Kent, UK)
Thursday 21 August 2008
09:00 - 09:40
The machine's smallest cog: unpaired electrons as probes of enzyme structure and function
Steve Rigby (Manchester Interdisciplinary Biocentre, UK)
09:40 - 10:20
Role of metals in E. coli copper amine oxidase
Michael McPherson (University of Leeds, UK)
10:20 - 11:00
C-H bond activation in heme proteins
Mike Green (Pennsylvania State University, USA)
11:00 - 11:30 Coffee/tea break

11:30 - 12:10
The DMGO-family, ¿simple¿ substrate channelling in a ¿primitive¿ enzyme?
David Leys (University of Manchester, UK)
12:10 - 12:50
Folding and unfolding by chaperone machines
Helen Saibil (Birkbeck College, University of London, UK)