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55th Harden Conference - Dynamics of Membrane Traffic

25—30 August 2002

St Martin's College, Ambleside, Lake District, UK



Coffee

Timetable

Monday 26 August 2002Tuesday 27 August 2002Wednesday 28 August 2002Thursday 29 August 2002
09:00 - 17:20
Cytoskeletal motors and membrane traffic

09:00 - 17:20
Golgi apparatus: transport and sorting

09:00 - 17:20
Traffic out of the Golgi apparatus

09:00 - 17:20
Dynamics of exocytosis

09:00 - 17:20
The endocytic pathway

09:00 - 17:20
The cytoskeleton and cell polarity

09:00 - 12:20
Cytoskeletal motors and membrane traffic

09:00 - 17:30
Golgi apparatus: transport and sorting

09:00 - 17:30
Traffic out of the Golgi apparatus

09:00 - 12:20
Dynamics of exocytosis

09:00 - 17:30
The endocytic pathway

09:00 - 12:20
The cytoskeleton and cell polarity

09:00 - 12:20
Cytoskeletal motors and membrane traffic

09:00 - 12:20
Golgi apparatus: transport and sorting

09:00 - 21:00
Traffic out of the Golgi apparatus

09:00 - 12:20
Dynamics of exocytosis

09:00 - 12:20
The endocytic pathway

09:00 - 12:20
The cytoskeleton and cell polarity

09:00 - 17:20
Cytoskeletal motors and membrane traffic

09:00 - 17:20
Golgi apparatus: transport and sorting

09:00 - 17:20
Traffic out of the Golgi apparatus

09:00 - 17:20
Dynamics of exocytosis

09:00 - 17:20
The endocytic pathway

09:00 - 17:20
The cytoskeleton and cell polarity


Cytoskeletal motors and membrane traffic

Organizers:
Viki Allan (Manchester, UK)
Bob Burgoyne (Liverpool, UK)
The Company of Biologists Limited
Trends in Cell Biology
Tuesday 27 August 2002
09:00 - 09:40
Temporal and spatial organization of membrane traffic at the ER/Golgi boundary
Rainer Pepperkok (Heidelberg, Germany)
09:40 - 10:00
GRASP-golgin complexes are rab-GTPase effectors required for Golgi function and structure
Francis Barr (Max Planck Institute, Martinsried, Germany)
10:20 - 11:00 Coffee

11:00 - 11:40
Membrane protein sorting in yeast
Hugh Pelham (Cambridge, UK)
11:40 - 12:20
The role of Rab6 and Rab11 in the retrograde transport pathway between endosomes and the endoplasmic reticulum
Bruno Goud (Institut Curie, Paris, France)
12:20 - 14:00 Lunch and poster viewing

Wednesday 28 August 2002
09:00 - 09:40
Traffic within and beyond the TGN: molecules and movies
George Banting (Bristol, UK)
09:40 - 10:20
Endocytosis and intracellular transport of protein toxins
Kirsten Sandvig (Institute of Cancer Research, Oslo, Norway)
10:20 - 11:00 Coffee

11:00 - 11:40
TBA
11:40 - 12:20
Spatial regulation of exocytosis
Peter Novick (Trends in Cell Biology lecture) (Yale, CT, USA)
12:20 - 14:00 Lunch and poster viewing

Thursday 29 August 2002
09:00 - 09:40
Molecular mechanisms mediating calcium control of neurotransmitter release
Weiping Han (Dallas, TX, USA)
09:40 - 10:20
The role of snares and their regulators in membrane fusion dynamics during regulated exocytosis
Bob Burgoyne (Liverpool, UK)
10:20 - 11:00 Coffee

11:00 - 11:40
Molecular mechanisms of insulin secretory vesicle movement and exocytosis
Guy Rutter (Bristol, UK)
11:40 - 11:55
Conformational regulation of SNARE assembly and disassembly
Selected Poster Presentation - Mary Munson (UMass Medical School, Worcester, MA, USA)
11:55 - 12:10
The Ca2+ binding function of the C2B, but not the C2A domain of Drosophila synaptotagmin is essential for neurotransmitter release
Selected Poster Presentation - Iain Robinson (Cambridge, UK)
12:10 - 12:25
Mechanism of calcium action in synaptic vesicle exocytosis
Selected Poster Presentation - Kuang Hu (MRC, LMB, Cambridge, UK)
12:25 - 14:00 Lunch and poster viewing

14:00 - 14:15
Phosphatidylinositol 4-OH kinase is a downstream target of neuronal calcium sensor-1 enhancing exocytosis in neuroendocrine cells
Selected Poster Presentation - Sabine Hilfiker (Manchester, UK)
14:15 - 14:30
Ysc84p and Lsb5p link the actin cytoskeleton to the endocytic machinery in Saccharomyes Cerevisiae
Selected Poster Presentation - Kathryn Ayscough (Glasgow, UK)
14:30 - 14:45
Dual Arf/Rab11 effector proteins that play a role in cytokinesis and regulate distribution of the endosomal recycling compartment
Selected Poster Presentation - Johanne Matheson
14:40 - 15:20
Traffic to and through early endosomes
Judith Klumperman (Utrecht, Netherlands)
15:20 - 16:00 Coffee

16:00 - 16:40
Rab-domains in endosome organization and function
Marino Zerial (Max Plank Institute, Dresden, Germany)
16:40 - 17:20
Driving membrane curvature during endocytosis
Harvey McMahon (Cambridge, UK)

Morning

Coffee

Monday 26 August 2002
09:00 - 09:40
Microtubule motors in the early secretory pathway
Viki Allan (Manchester, UK)
09:40 - 10:20
Regulation and coordination of molecular motors
Vladimir Gelfand (Urbana, IL, USA)
10:20 - 11:00 Coffee

11:00 - 11:40
Dual control of Caveolin-1 trafficking by microtubules and actin filaments
George Bloom (Charlottesville, VA, USA)
11:40 - 12:20
Myosin VI, a dynamic link between membrane traffic and the actin cytoskeleton
Folma Buss (Cambridge, UK)
12:20 - 14:00 Lunch and poster viewing

14:00 - 14:40
Kinesin based transport pathways in signalling and disease
Larry Goldstein (The Company of Biologists Lecture) (San Diego, CA, USA)
14:40 - 15:20
Dynein, dynactin and cytoplasmic organization
Trina Schroer (Johns Hopkins, MD, USA)
15:20 - 16:00 Coffee

16:00 - 16:40
Role of adaptors and microtubule motors in polarized post Golgi trafficking
Enrique Rodriguez-Boulan (Cornell, NY, USA)
16:40 - 17:20
Studies on the morpho-functional organization of the secretory pathway in living cells
Alberto Luini (Consorzio Mario Negri Institute, Santa Maria Imbaro, Italy)
18:30 Dinner

The cytoskeleton and cell polarity

Tuesday 27 August 2002
09:00 - 09:40
Temporal and spatial organization of membrane traffic at the ER/Golgi boundary
Rainer Pepperkok (Heidelberg, Germany)
09:40 - 10:00
GRASP-golgin complexes are rab-GTPase effectors required for Golgi function and structure
Francis Barr (Max Planck Institute, Martinsried, Germany)
10:20 - 11:00 Coffee

11:00 - 11:40
Membrane protein sorting in yeast
Hugh Pelham (Cambridge, UK)
11:40 - 12:20
The role of Rab6 and Rab11 in the retrograde transport pathway between endosomes and the endoplasmic reticulum
Bruno Goud (Institut Curie, Paris, France)
12:20 - 14:00 Lunch and poster viewing

Wednesday 28 August 2002
09:00 - 09:40
Traffic within and beyond the TGN: molecules and movies
George Banting (Bristol, UK)
09:40 - 10:20
Endocytosis and intracellular transport of protein toxins
Kirsten Sandvig (Institute of Cancer Research, Oslo, Norway)
10:20 - 11:00 Coffee

11:00 - 11:40
TBA
11:40 - 12:20
Spatial regulation of exocytosis
Peter Novick (Trends in Cell Biology lecture) (Yale, CT, USA)
12:20 - 14:00 Lunch and poster viewing

Thursday 29 August 2002
09:00 - 09:40
Molecular mechanisms mediating calcium control of neurotransmitter release
Weiping Han (Dallas, TX, USA)
09:40 - 10:20
The role of snares and their regulators in membrane fusion dynamics during regulated exocytosis
Bob Burgoyne (Liverpool, UK)
10:20 - 11:00 Coffee

11:00 - 11:40
Molecular mechanisms of insulin secretory vesicle movement and exocytosis
Guy Rutter (Bristol, UK)
11:40 - 11:55
Conformational regulation of SNARE assembly and disassembly
Selected Poster Presentation - Mary Munson (UMass Medical School, Worcester, MA, USA)
11:55 - 12:10
The Ca2+ binding function of the C2B, but not the C2A domain of Drosophila synaptotagmin is essential for neurotransmitter release
Selected Poster Presentation - Iain Robinson (Cambridge, UK)
12:10 - 12:25
Mechanism of calcium action in synaptic vesicle exocytosis
Selected Poster Presentation - Kuang Hu (MRC, LMB, Cambridge, UK)
12:25 - 14:00 Lunch and poster viewing

14:00 - 14:15
Phosphatidylinositol 4-OH kinase is a downstream target of neuronal calcium sensor-1 enhancing exocytosis in neuroendocrine cells
Selected Poster Presentation - Sabine Hilfiker (Manchester, UK)
14:15 - 14:30
Ysc84p and Lsb5p link the actin cytoskeleton to the endocytic machinery in Saccharomyes Cerevisiae
Selected Poster Presentation - Kathryn Ayscough (Glasgow, UK)
14:30 - 14:45
Dual Arf/Rab11 effector proteins that play a role in cytokinesis and regulate distribution of the endosomal recycling compartment
Selected Poster Presentation - Johanne Matheson
14:40 - 15:20
Traffic to and through early endosomes
Judith Klumperman (Utrecht, Netherlands)
15:20 - 16:00 Coffee

16:00 - 16:40
Rab-domains in endosome organization and function
Marino Zerial (Max Plank Institute, Dresden, Germany)
16:40 - 17:20
Driving membrane curvature during endocytosis
Harvey McMahon (Cambridge, UK)

Afternoon

Coffee

Monday 26 August 2002
09:00 - 09:40
Microtubule motors in the early secretory pathway
Viki Allan (Manchester, UK)
09:40 - 10:20
Regulation and coordination of molecular motors
Vladimir Gelfand (Urbana, IL, USA)
10:20 - 11:00 Coffee

11:00 - 11:40
Dual control of Caveolin-1 trafficking by microtubules and actin filaments
George Bloom (Charlottesville, VA, USA)
11:40 - 12:20
Myosin VI, a dynamic link between membrane traffic and the actin cytoskeleton
Folma Buss (Cambridge, UK)
12:20 - 14:00 Lunch and poster viewing

14:00 - 14:40
Kinesin based transport pathways in signalling and disease
Larry Goldstein (The Company of Biologists Lecture) (San Diego, CA, USA)
14:40 - 15:20
Dynein, dynactin and cytoplasmic organization
Trina Schroer (Johns Hopkins, MD, USA)
15:20 - 16:00 Coffee

16:00 - 16:40
Role of adaptors and microtubule motors in polarized post Golgi trafficking
Enrique Rodriguez-Boulan (Cornell, NY, USA)
16:40 - 17:20
Studies on the morpho-functional organization of the secretory pathway in living cells
Alberto Luini (Consorzio Mario Negri Institute, Santa Maria Imbaro, Italy)
18:30 Dinner

Golgi apparatus: transport and sorting

Monday 26 August 2002
09:00 - 09:40
Microtubule motors in the early secretory pathway
Viki Allan (Manchester, UK)
09:40 - 10:20
Regulation and coordination of molecular motors
Vladimir Gelfand (Urbana, IL, USA)
10:20 - 11:00 Coffee

11:00 - 11:40
Dual control of Caveolin-1 trafficking by microtubules and actin filaments
George Bloom (Charlottesville, VA, USA)
11:40 - 12:20
Myosin VI, a dynamic link between membrane traffic and the actin cytoskeleton
Folma Buss (Cambridge, UK)
12:20 - 14:00 Lunch and poster viewing

14:00 - 14:40
Kinesin based transport pathways in signalling and disease
Larry Goldstein (The Company of Biologists Lecture) (San Diego, CA, USA)
14:40 - 15:20
Dynein, dynactin and cytoplasmic organization
Trina Schroer (Johns Hopkins, MD, USA)
15:20 - 16:00 Coffee

16:00 - 16:40
Role of adaptors and microtubule motors in polarized post Golgi trafficking
Enrique Rodriguez-Boulan (Cornell, NY, USA)
16:40 - 17:20
Studies on the morpho-functional organization of the secretory pathway in living cells
Alberto Luini (Consorzio Mario Negri Institute, Santa Maria Imbaro, Italy)
18:30 Dinner

Wednesday 28 August 2002
09:00 - 09:40
Traffic within and beyond the TGN: molecules and movies
George Banting (Bristol, UK)
09:40 - 10:20
Endocytosis and intracellular transport of protein toxins
Kirsten Sandvig (Institute of Cancer Research, Oslo, Norway)
10:20 - 11:00 Coffee

11:00 - 11:40
TBA
11:40 - 12:20
Spatial regulation of exocytosis
Peter Novick (Trends in Cell Biology lecture) (Yale, CT, USA)
12:20 - 14:00 Lunch and poster viewing

14:00 - 17:00 Free Afternoon

18:30 - 20:00 Dinner

Thursday 29 August 2002
09:00 - 09:40
Molecular mechanisms mediating calcium control of neurotransmitter release
Weiping Han (Dallas, TX, USA)
09:40 - 10:20
The role of snares and their regulators in membrane fusion dynamics during regulated exocytosis
Bob Burgoyne (Liverpool, UK)
10:20 - 11:00 Coffee

11:00 - 11:40
Molecular mechanisms of insulin secretory vesicle movement and exocytosis
Guy Rutter (Bristol, UK)
11:40 - 11:55
Conformational regulation of SNARE assembly and disassembly
Selected Poster Presentation - Mary Munson (UMass Medical School, Worcester, MA, USA)
11:55 - 12:10
The Ca2+ binding function of the C2B, but not the C2A domain of Drosophila synaptotagmin is essential for neurotransmitter release
Selected Poster Presentation - Iain Robinson (Cambridge, UK)
12:10 - 12:25
Mechanism of calcium action in synaptic vesicle exocytosis
Selected Poster Presentation - Kuang Hu (MRC, LMB, Cambridge, UK)
12:25 - 14:00 Lunch and poster viewing

14:00 - 14:15
Phosphatidylinositol 4-OH kinase is a downstream target of neuronal calcium sensor-1 enhancing exocytosis in neuroendocrine cells
Selected Poster Presentation - Sabine Hilfiker (Manchester, UK)
14:15 - 14:30
Ysc84p and Lsb5p link the actin cytoskeleton to the endocytic machinery in Saccharomyes Cerevisiae
Selected Poster Presentation - Kathryn Ayscough (Glasgow, UK)
14:30 - 14:45
Dual Arf/Rab11 effector proteins that play a role in cytokinesis and regulate distribution of the endosomal recycling compartment
Selected Poster Presentation - Johanne Matheson
14:40 - 15:20
Traffic to and through early endosomes
Judith Klumperman (Utrecht, Netherlands)
15:20 - 16:00 Coffee

16:00 - 16:40
Rab-domains in endosome organization and function
Marino Zerial (Max Plank Institute, Dresden, Germany)
16:40 - 17:20
Driving membrane curvature during endocytosis
Harvey McMahon (Cambridge, UK)
19:00 Conference Dinner

Morning

Coffee

Tuesday 27 August 2002
09:00 - 09:40
Temporal and spatial organization of membrane traffic at the ER/Golgi boundary
Rainer Pepperkok (Heidelberg, Germany)
09:40 - 10:00
GRASP-golgin complexes are rab-GTPase effectors required for Golgi function and structure
Francis Barr (Max Planck Institute, Martinsried, Germany)
10:20 - 11:00 Coffee

11:00 - 11:40
Membrane protein sorting in yeast
Hugh Pelham (Cambridge, UK)
11:40 - 12:20
The role of Rab6 and Rab11 in the retrograde transport pathway between endosomes and the endoplasmic reticulum
Bruno Goud (Institut Curie, Paris, France)
12:20 - 14:00 Lunch and poster viewing

Afternoon

Coffee

Tuesday 27 August 2002
14:00 - 14:15
Cargo sorting during ER-to-Golgi transport
Selected Poster Presentation - David Stephens (Bristol, UK)
14:15 - 14:30
Identification and characterization of a conserved Golgi membrane protein related to giantin and golgi-84
Selected Poster Presentation - Alison Gillingham (MRC, LMB, Cambridge, UK)
14:30 - 14:45
Glycosylation determinants for endolyn sorting in polarized MDCK cells
Selected Poster Presentation - Ora Weisz (Pittsburgh, PA, USA)
14:45 - 15:00
Intracellular localization of Fv1, a murine retroviral resistance gene
Selected Poster Presentation - Melvyn Yap (NIMR, London)
15:00 - 15:15
DAMP-1 is a lipid raft associated with a novel topology
Selected Poster Presentation - Viktor Korolchuk (Bristol, UK)
15:15 - 15:30
A feed-forward loop between PI-3,4,5-P3 generation and actin remodelling is required for enhancing GLUT4 traffic by insulin skeletal muscle cells
Selected Poster Presentation - Assaf Rudich (Hospital for Sick Children, Ontario, Canada)
15:30 - 16:00 Coffee

16:00 - 16:15
Rab and Rho effector complexes in plants
Selected Poster Presentation - Viktor Zarsky (Institute Of Experimental Botany, Prague, Czech Republic)
16:15 - 16:30
Regulation of exocytotic activity by synthesis of secretory protein and ion channels
Selected Poster Presentation - Jens - Uwe Sutter (IBLS, Glasgow, UK)
16:30 - 16:45
TPA-induced macropinocytotis require cholesterol
Selected Poster Presentation - Stine Grimmer (The Norwegian Radium Hospital, Oslo, Norway)
16:45 - 17:00
Human NK cell CD94/NKG2A inhibitor receptors are internalized and recycle independently of the inhibitory signaling process
Selected Poster Presentation - Francisco Borrego (NIAID, NIH, Rockville, MD, USA)
17:00 - 17:15
Synapsin I-associated phosphatidylinositol 3-kinase activity is required for maintenance of exocytosis in nerve terminals
Selected Poster Presentation - Mike Cousin (Edinburgh, UK)
17:15 - 17:30
Membrane tubulovesicular extensions of neutrophils can represent protusions of an exocytotic cell machinery and serve as celluar probing and adhesive organelles
Selected Poster Presentation - Svetlana Galkina (Moscow, Russia)
18:30 Dinner

Traffic out of the Golgi apparatus

Monday 26 August 2002
09:00 - 09:40
Microtubule motors in the early secretory pathway
Viki Allan (Manchester, UK)
09:40 - 10:20
Regulation and coordination of molecular motors
Vladimir Gelfand (Urbana, IL, USA)
10:20 - 11:00 Coffee

11:00 - 11:40
Dual control of Caveolin-1 trafficking by microtubules and actin filaments
George Bloom (Charlottesville, VA, USA)
11:40 - 12:20
Myosin VI, a dynamic link between membrane traffic and the actin cytoskeleton
Folma Buss (Cambridge, UK)
12:20 - 14:00 Lunch and poster viewing

14:00 - 14:40
Kinesin based transport pathways in signalling and disease
Larry Goldstein (The Company of Biologists Lecture) (San Diego, CA, USA)
14:40 - 15:20
Dynein, dynactin and cytoplasmic organization
Trina Schroer (Johns Hopkins, MD, USA)
15:20 - 16:00 Coffee

16:00 - 16:40
Role of adaptors and microtubule motors in polarized post Golgi trafficking
Enrique Rodriguez-Boulan (Cornell, NY, USA)
16:40 - 17:20
Studies on the morpho-functional organization of the secretory pathway in living cells
Alberto Luini (Consorzio Mario Negri Institute, Santa Maria Imbaro, Italy)
18:30 Dinner

Tuesday 27 August 2002
09:00 - 09:40
Temporal and spatial organization of membrane traffic at the ER/Golgi boundary
Rainer Pepperkok (Heidelberg, Germany)
09:40 - 10:00
GRASP-golgin complexes are rab-GTPase effectors required for Golgi function and structure
Francis Barr (Max Planck Institute, Martinsried, Germany)
10:20 - 11:00 Coffee

11:00 - 11:40
Membrane protein sorting in yeast
Hugh Pelham (Cambridge, UK)
11:40 - 12:20
The role of Rab6 and Rab11 in the retrograde transport pathway between endosomes and the endoplasmic reticulum
Bruno Goud (Institut Curie, Paris, France)
12:20 - 14:00 Lunch and poster viewing

14:00 - 14:15
Cargo sorting during ER-to-Golgi transport
Selected Poster Presentation - David Stephens (Bristol, UK)
14:15 - 14:30
Identification and characterization of a conserved Golgi membrane protein related to giantin and golgi-84
Selected Poster Presentation - Alison Gillingham (MRC, LMB, Cambridge, UK)
14:30 - 14:45
Glycosylation determinants for endolyn sorting in polarized MDCK cells
Selected Poster Presentation - Ora Weisz (Pittsburgh, PA, USA)
14:45 - 15:00
Intracellular localization of Fv1, a murine retroviral resistance gene
Selected Poster Presentation - Melvyn Yap (NIMR, London)
15:00 - 15:15
DAMP-1 is a lipid raft associated with a novel topology
Selected Poster Presentation - Viktor Korolchuk (Bristol, UK)
15:15 - 15:30
A feed-forward loop between PI-3,4,5-P3 generation and actin remodelling is required for enhancing GLUT4 traffic by insulin skeletal muscle cells
Selected Poster Presentation - Assaf Rudich (Hospital for Sick Children, Ontario, Canada)
15:30 - 16:00 Coffee

16:00 - 16:15
Rab and Rho effector complexes in plants
Selected Poster Presentation - Viktor Zarsky (Institute Of Experimental Botany, Prague, Czech Republic)
16:15 - 16:30
Regulation of exocytotic activity by synthesis of secretory protein and ion channels
Selected Poster Presentation - Jens - Uwe Sutter (IBLS, Glasgow, UK)
16:30 - 16:45
TPA-induced macropinocytotis require cholesterol
Selected Poster Presentation - Stine Grimmer (The Norwegian Radium Hospital, Oslo, Norway)
16:45 - 17:00
Human NK cell CD94/NKG2A inhibitor receptors are internalized and recycle independently of the inhibitory signaling process
Selected Poster Presentation - Francisco Borrego (NIAID, NIH, Rockville, MD, USA)
17:00 - 17:15
Synapsin I-associated phosphatidylinositol 3-kinase activity is required for maintenance of exocytosis in nerve terminals
Selected Poster Presentation - Mike Cousin (Edinburgh, UK)
17:15 - 17:30
Membrane tubulovesicular extensions of neutrophils can represent protusions of an exocytotic cell machinery and serve as celluar probing and adhesive organelles
Selected Poster Presentation - Svetlana Galkina (Moscow, Russia)
18:30 Dinner

Thursday 29 August 2002
09:00 - 09:40
Molecular mechanisms mediating calcium control of neurotransmitter release
Weiping Han (Dallas, TX, USA)
09:40 - 10:20
The role of snares and their regulators in membrane fusion dynamics during regulated exocytosis
Bob Burgoyne (Liverpool, UK)
10:20 - 11:00 Coffee

11:00 - 11:40
Molecular mechanisms of insulin secretory vesicle movement and exocytosis
Guy Rutter (Bristol, UK)
11:40 - 11:55
Conformational regulation of SNARE assembly and disassembly
Selected Poster Presentation - Mary Munson (UMass Medical School, Worcester, MA, USA)
11:55 - 12:10
The Ca2+ binding function of the C2B, but not the C2A domain of Drosophila synaptotagmin is essential for neurotransmitter release
Selected Poster Presentation - Iain Robinson (Cambridge, UK)
12:10 - 12:25
Mechanism of calcium action in synaptic vesicle exocytosis
Selected Poster Presentation - Kuang Hu (MRC, LMB, Cambridge, UK)
12:25 - 14:00 Lunch and poster viewing

14:00 - 14:15
Phosphatidylinositol 4-OH kinase is a downstream target of neuronal calcium sensor-1 enhancing exocytosis in neuroendocrine cells
Selected Poster Presentation - Sabine Hilfiker (Manchester, UK)
14:15 - 14:30
Ysc84p and Lsb5p link the actin cytoskeleton to the endocytic machinery in Saccharomyes Cerevisiae
Selected Poster Presentation - Kathryn Ayscough (Glasgow, UK)
14:30 - 14:45
Dual Arf/Rab11 effector proteins that play a role in cytokinesis and regulate distribution of the endosomal recycling compartment
Selected Poster Presentation - Johanne Matheson
14:40 - 15:20
Traffic to and through early endosomes
Judith Klumperman (Utrecht, Netherlands)
15:20 - 16:00 Coffee

16:00 - 16:40
Rab-domains in endosome organization and function
Marino Zerial (Max Plank Institute, Dresden, Germany)
16:40 - 17:20
Driving membrane curvature during endocytosis
Harvey McMahon (Cambridge, UK)
19:00 Conference Dinner

Morning

Coffee

Wednesday 28 August 2002
09:00 - 09:40
Traffic within and beyond the TGN: molecules and movies
George Banting (Bristol, UK)
09:40 - 10:20
Endocytosis and intracellular transport of protein toxins
Kirsten Sandvig (Institute of Cancer Research, Oslo, Norway)
10:20 - 11:00 Coffee

11:00 - 11:40
TBA
11:40 - 12:20
Spatial regulation of exocytosis
Peter Novick (Trends in Cell Biology lecture) (Yale, CT, USA)
12:20 - 14:00 Lunch and poster viewing

Afternoon

Coffee

Wednesday 28 August 2002
14:00 - 17:00 Free Afternoon

18:30 - 20:00 Dinner

Harden lecture (evening)

Coffee

Wednesday 28 August 2002
20:00 - 21:00
Biogenesis of the Golgi apparatus
Graham Warren (Yale, CT, USA)
21:00 Poster session

Dynamics of exocytosis

Monday 26 August 2002
09:00 - 09:40
Microtubule motors in the early secretory pathway
Viki Allan (Manchester, UK)
09:40 - 10:20
Regulation and coordination of molecular motors
Vladimir Gelfand (Urbana, IL, USA)
10:20 - 11:00 Coffee

11:00 - 11:40
Dual control of Caveolin-1 trafficking by microtubules and actin filaments
George Bloom (Charlottesville, VA, USA)
11:40 - 12:20
Myosin VI, a dynamic link between membrane traffic and the actin cytoskeleton
Folma Buss (Cambridge, UK)
12:20 - 14:00 Lunch and poster viewing

14:00 - 14:40
Kinesin based transport pathways in signalling and disease
Larry Goldstein (The Company of Biologists Lecture) (San Diego, CA, USA)
14:40 - 15:20
Dynein, dynactin and cytoplasmic organization
Trina Schroer (Johns Hopkins, MD, USA)
15:20 - 16:00 Coffee

16:00 - 16:40
Role of adaptors and microtubule motors in polarized post Golgi trafficking
Enrique Rodriguez-Boulan (Cornell, NY, USA)
16:40 - 17:20
Studies on the morpho-functional organization of the secretory pathway in living cells
Alberto Luini (Consorzio Mario Negri Institute, Santa Maria Imbaro, Italy)
18:30 Dinner

Tuesday 27 August 2002
09:00 - 09:40
Temporal and spatial organization of membrane traffic at the ER/Golgi boundary
Rainer Pepperkok (Heidelberg, Germany)
09:40 - 10:00
GRASP-golgin complexes are rab-GTPase effectors required for Golgi function and structure
Francis Barr (Max Planck Institute, Martinsried, Germany)
10:20 - 11:00 Coffee

11:00 - 11:40
Membrane protein sorting in yeast
Hugh Pelham (Cambridge, UK)
11:40 - 12:20
The role of Rab6 and Rab11 in the retrograde transport pathway between endosomes and the endoplasmic reticulum
Bruno Goud (Institut Curie, Paris, France)
12:20 - 14:00 Lunch and poster viewing

Wednesday 28 August 2002
09:00 - 09:40
Traffic within and beyond the TGN: molecules and movies
George Banting (Bristol, UK)
09:40 - 10:20
Endocytosis and intracellular transport of protein toxins
Kirsten Sandvig (Institute of Cancer Research, Oslo, Norway)
10:20 - 11:00 Coffee

11:00 - 11:40
TBA
11:40 - 12:20
Spatial regulation of exocytosis
Peter Novick (Trends in Cell Biology lecture) (Yale, CT, USA)
12:20 - 14:00 Lunch and poster viewing

Morning

Coffee

Thursday 29 August 2002
09:00 - 09:40
Molecular mechanisms mediating calcium control of neurotransmitter release
Weiping Han (Dallas, TX, USA)
09:40 - 10:20
The role of snares and their regulators in membrane fusion dynamics during regulated exocytosis
Bob Burgoyne (Liverpool, UK)
10:20 - 11:00 Coffee

11:00 - 11:40
Molecular mechanisms of insulin secretory vesicle movement and exocytosis
Guy Rutter (Bristol, UK)
11:40 - 11:55
Conformational regulation of SNARE assembly and disassembly
Selected Poster Presentation - Mary Munson (UMass Medical School, Worcester, MA, USA)
11:55 - 12:10
The Ca2+ binding function of the C2B, but not the C2A domain of Drosophila synaptotagmin is essential for neurotransmitter release
Selected Poster Presentation - Iain Robinson (Cambridge, UK)
12:10 - 12:25
Mechanism of calcium action in synaptic vesicle exocytosis
Selected Poster Presentation - Kuang Hu (MRC, LMB, Cambridge, UK)
12:25 - 14:00 Lunch and poster viewing

14:00 - 14:15
Phosphatidylinositol 4-OH kinase is a downstream target of neuronal calcium sensor-1 enhancing exocytosis in neuroendocrine cells
Selected Poster Presentation - Sabine Hilfiker (Manchester, UK)
14:15 - 14:30
Ysc84p and Lsb5p link the actin cytoskeleton to the endocytic machinery in Saccharomyes Cerevisiae
Selected Poster Presentation - Kathryn Ayscough (Glasgow, UK)
14:30 - 14:45
Dual Arf/Rab11 effector proteins that play a role in cytokinesis and regulate distribution of the endosomal recycling compartment
Selected Poster Presentation - Johanne Matheson
14:40 - 15:20
Traffic to and through early endosomes
Judith Klumperman (Utrecht, Netherlands)
15:20 - 16:00 Coffee

16:00 - 16:40
Rab-domains in endosome organization and function
Marino Zerial (Max Plank Institute, Dresden, Germany)
16:40 - 17:20
Driving membrane curvature during endocytosis
Harvey McMahon (Cambridge, UK)

The endocytic pathway

Monday 26 August 2002
09:00 - 09:40
Microtubule motors in the early secretory pathway
Viki Allan (Manchester, UK)
09:40 - 10:20
Regulation and coordination of molecular motors
Vladimir Gelfand (Urbana, IL, USA)
10:20 - 11:00 Coffee

11:00 - 11:40
Dual control of Caveolin-1 trafficking by microtubules and actin filaments
George Bloom (Charlottesville, VA, USA)
11:40 - 12:20
Myosin VI, a dynamic link between membrane traffic and the actin cytoskeleton
Folma Buss (Cambridge, UK)
12:20 - 14:00 Lunch and poster viewing

14:00 - 14:40
Kinesin based transport pathways in signalling and disease
Larry Goldstein (The Company of Biologists Lecture) (San Diego, CA, USA)
14:40 - 15:20
Dynein, dynactin and cytoplasmic organization
Trina Schroer (Johns Hopkins, MD, USA)
15:20 - 16:00 Coffee

16:00 - 16:40
Role of adaptors and microtubule motors in polarized post Golgi trafficking
Enrique Rodriguez-Boulan (Cornell, NY, USA)
16:40 - 17:20
Studies on the morpho-functional organization of the secretory pathway in living cells
Alberto Luini (Consorzio Mario Negri Institute, Santa Maria Imbaro, Italy)
18:30 Dinner

Tuesday 27 August 2002
09:00 - 09:40
Temporal and spatial organization of membrane traffic at the ER/Golgi boundary
Rainer Pepperkok (Heidelberg, Germany)
09:40 - 10:00
GRASP-golgin complexes are rab-GTPase effectors required for Golgi function and structure
Francis Barr (Max Planck Institute, Martinsried, Germany)
10:20 - 11:00 Coffee

11:00 - 11:40
Membrane protein sorting in yeast
Hugh Pelham (Cambridge, UK)
11:40 - 12:20
The role of Rab6 and Rab11 in the retrograde transport pathway between endosomes and the endoplasmic reticulum
Bruno Goud (Institut Curie, Paris, France)
12:20 - 14:00 Lunch and poster viewing

14:00 - 14:15
Cargo sorting during ER-to-Golgi transport
Selected Poster Presentation - David Stephens (Bristol, UK)
14:15 - 14:30
Identification and characterization of a conserved Golgi membrane protein related to giantin and golgi-84
Selected Poster Presentation - Alison Gillingham (MRC, LMB, Cambridge, UK)
14:30 - 14:45
Glycosylation determinants for endolyn sorting in polarized MDCK cells
Selected Poster Presentation - Ora Weisz (Pittsburgh, PA, USA)
14:45 - 15:00
Intracellular localization of Fv1, a murine retroviral resistance gene
Selected Poster Presentation - Melvyn Yap (NIMR, London)
15:00 - 15:15
DAMP-1 is a lipid raft associated with a novel topology
Selected Poster Presentation - Viktor Korolchuk (Bristol, UK)
15:15 - 15:30
A feed-forward loop between PI-3,4,5-P3 generation and actin remodelling is required for enhancing GLUT4 traffic by insulin skeletal muscle cells
Selected Poster Presentation - Assaf Rudich (Hospital for Sick Children, Ontario, Canada)
15:30 - 16:00 Coffee

16:00 - 16:15
Rab and Rho effector complexes in plants
Selected Poster Presentation - Viktor Zarsky (Institute Of Experimental Botany, Prague, Czech Republic)
16:15 - 16:30
Regulation of exocytotic activity by synthesis of secretory protein and ion channels
Selected Poster Presentation - Jens - Uwe Sutter (IBLS, Glasgow, UK)
16:30 - 16:45
TPA-induced macropinocytotis require cholesterol
Selected Poster Presentation - Stine Grimmer (The Norwegian Radium Hospital, Oslo, Norway)
16:45 - 17:00
Human NK cell CD94/NKG2A inhibitor receptors are internalized and recycle independently of the inhibitory signaling process
Selected Poster Presentation - Francisco Borrego (NIAID, NIH, Rockville, MD, USA)
17:00 - 17:15
Synapsin I-associated phosphatidylinositol 3-kinase activity is required for maintenance of exocytosis in nerve terminals
Selected Poster Presentation - Mike Cousin (Edinburgh, UK)
17:15 - 17:30
Membrane tubulovesicular extensions of neutrophils can represent protusions of an exocytotic cell machinery and serve as celluar probing and adhesive organelles
Selected Poster Presentation - Svetlana Galkina (Moscow, Russia)
18:30 Dinner

Wednesday 28 August 2002
09:00 - 09:40
Traffic within and beyond the TGN: molecules and movies
George Banting (Bristol, UK)
09:40 - 10:20
Endocytosis and intracellular transport of protein toxins
Kirsten Sandvig (Institute of Cancer Research, Oslo, Norway)
10:20 - 11:00 Coffee

11:00 - 11:40
TBA
11:40 - 12:20
Spatial regulation of exocytosis
Peter Novick (Trends in Cell Biology lecture) (Yale, CT, USA)
12:20 - 14:00 Lunch and poster viewing

14:00 - 17:00 Free Afternoon

18:30 - 20:00 Dinner

Afternoon

Coffee

Thursday 29 August 2002
09:00 - 09:40
Molecular mechanisms mediating calcium control of neurotransmitter release
Weiping Han (Dallas, TX, USA)
09:40 - 10:20
The role of snares and their regulators in membrane fusion dynamics during regulated exocytosis
Bob Burgoyne (Liverpool, UK)
10:20 - 11:00 Coffee

11:00 - 11:40
Molecular mechanisms of insulin secretory vesicle movement and exocytosis
Guy Rutter (Bristol, UK)
11:40 - 11:55
Conformational regulation of SNARE assembly and disassembly
Selected Poster Presentation - Mary Munson (UMass Medical School, Worcester, MA, USA)
11:55 - 12:10
The Ca2+ binding function of the C2B, but not the C2A domain of Drosophila synaptotagmin is essential for neurotransmitter release
Selected Poster Presentation - Iain Robinson (Cambridge, UK)
12:10 - 12:25
Mechanism of calcium action in synaptic vesicle exocytosis
Selected Poster Presentation - Kuang Hu (MRC, LMB, Cambridge, UK)
12:25 - 14:00 Lunch and poster viewing

14:00 - 14:15
Phosphatidylinositol 4-OH kinase is a downstream target of neuronal calcium sensor-1 enhancing exocytosis in neuroendocrine cells
Selected Poster Presentation - Sabine Hilfiker (Manchester, UK)
14:15 - 14:30
Ysc84p and Lsb5p link the actin cytoskeleton to the endocytic machinery in Saccharomyes Cerevisiae
Selected Poster Presentation - Kathryn Ayscough (Glasgow, UK)
14:30 - 14:45
Dual Arf/Rab11 effector proteins that play a role in cytokinesis and regulate distribution of the endosomal recycling compartment
Selected Poster Presentation - Johanne Matheson
14:40 - 15:20
Traffic to and through early endosomes
Judith Klumperman (Utrecht, Netherlands)
15:20 - 16:00 Coffee

16:00 - 16:40
Rab-domains in endosome organization and function
Marino Zerial (Max Plank Institute, Dresden, Germany)
16:40 - 17:20
Driving membrane curvature during endocytosis
Harvey McMahon (Cambridge, UK)

Evening

Coffee

Thursday 29 August 2002
09:00 - 09:40
Molecular mechanisms mediating calcium control of neurotransmitter release
Weiping Han (Dallas, TX, USA)
09:40 - 10:20
The role of snares and their regulators in membrane fusion dynamics during regulated exocytosis
Bob Burgoyne (Liverpool, UK)
10:20 - 11:00 Coffee

11:00 - 11:40
Molecular mechanisms of insulin secretory vesicle movement and exocytosis
Guy Rutter (Bristol, UK)
11:40 - 11:55
Conformational regulation of SNARE assembly and disassembly
Selected Poster Presentation - Mary Munson (UMass Medical School, Worcester, MA, USA)
11:55 - 12:10
The Ca2+ binding function of the C2B, but not the C2A domain of Drosophila synaptotagmin is essential for neurotransmitter release
Selected Poster Presentation - Iain Robinson (Cambridge, UK)
12:10 - 12:25
Mechanism of calcium action in synaptic vesicle exocytosis
Selected Poster Presentation - Kuang Hu (MRC, LMB, Cambridge, UK)
12:25 - 14:00 Lunch and poster viewing

19:00 Conference Dinner