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57th Harden Conference - Proteinase Structure and Function

9—13 September 2003

Oriel College, Oxford, UK



Proteinase Structure and Function

Organizers:
Robin Leatherbarrow (Imperial College London, UK)
Brian Austen (St. Georges Hospital Medical School, London, UK)
Chris Southan (Oxford Glycosciences, UK)
John Deadman (Thrombosis Research Institute, London, UK)
Sponsors:
Tuesday 9 September 2003
18:00 - 20:00 Registration

Wednesday morning

Chair:
Brian Austen (St. Georges Hospital Medical School, London, UK)
Wednesday 10 September 2003
08:55 - 09:00
Introduction and welcome
Brian Austen (St. Georges Hospital Medical School, London, UK)
09:00 - 09:45
Crystal structure of DegP (HtrA) reveals a new protease-chaperone machine
Tim Clausen (Institute for Molecular Pathology, Vienna, Austria)
09:45 - 10:30
Proteasome function in antigen presentation
Jennifer Rivett (Bristol, UK)
10:30 - 11:00 Coffee/tea break

11:00 - 11:45
Protease ligand conformation and inhibitor design
David Fairlie (Queensland, Australia)
11:45 - 11:55
Comparative modelling of angiotensin-converting enzyme-2 (ACE2)
Selected Poster Presentation - Jodie Guy (Leeds, UK)
11:55 - 12:05
An integrated platform for high throughput expression of human proteases
Selected Poster Presentation - Daniele Carettoni (Axxam, Italy)
12:05 - 12:15
FAK serves as a calpain protease-targeting adaptor molecule
Selected Poster Presentation - Neil Carragher (The Beatson Institute for Cancer Research, Glasgow, UK)
12:15 - 12:25
The crystal structure of the proprotein processing proteinase Furin
Selected Poster Presentation - Manuel Than (Max-Planck-Institut für Biochemie, Martinsried, Germany)
12:30 - 14:00 Lunch

Wednesday afternoon

Chair:
Christian Sommerhoff (Munich, Germany)
Wednesday 10 September 2003
12:30 - 14:00 Lunch

14:00 - 14:45
Bioinformatic analysis of the beta secretases, BACE1 and BACE2: integrating genome and transcript data from humans, rodents and fish
Chris Southan (Oxford Glycosciences, UK)
14:45 - 15:30
A presenilin dimer at the core of the g-secretase enzyme? Insights from parallel analysis of Notch 1 and APP proteolysis
Raphael Kopan (St Louis, MO, USA)
15:30 - 16:00 Coffee/tea break

16:00 - 16:45
Proteasome inhibitors induce apoptosis in human hepatoma cells by a c-Jun/JNK pathway
Sonia Emanuele (Palermo, Italy)
16:45 - 17:30
Chemical genetic and chemical biological applications of protease affinity labels
Brian Walker (Belfast, UK)
17:30 - 19:00 Drinks reception and poster viewing

19:00 Dinner

Thursday morning

Chair:
Chris Southan (Oxford Glycosciences, UK)
Thursday 11 September 2003
09:00 - 09:45
Structural studies on acyl enzyme complexes of serine proteases
Chris Schofield (Oxford, UK)
09:45 - 10:30
Zinc metalloproteinases as therapeutic targets in human disease
Anthony Turner (Leeds, UK)
10:30 - 11:00 Coffee/tea break

11:00 - 11:45
Intramembrane proteolysis by signal peptide peptidase
Bruno Martoglio (Swiss Federal Institute of Technology, Zurich, Switzerland)
11:45 - 12:30
The role of the apoptosome in caspase activation and cell death
Kelvin Cain (Leicester, UK)
12:30 - 14:00 Lunch

Thursday afternoon

Chair:
Anthony Turner (Leeds, UK)
Thursday 11 September 2003
12:30 - 14:00 Lunch

14:00 - 14:45
Subcellular trafficking and phosphorylation of endothelin-converting enzyme-1
Ian Smith (Cryptome Research, Baker Medical Institute, Melbourne, Australia)
14:45 - 15:30
Functional profiling of proteases: PNA-encoded small molecule probes
Jennifer Harris (Genomics Institute of the Novartis Research Foundation, San Diego, CA, USA)
15:30 - 17:30 Coffee/tea and poster session

19:00 Meeting dinner

Friday morning

Chair:
Jennifer Rivett (Bristol, UK)
Friday 12 September 2003
09:00 - 09:45
A mutagenesis approach to the understanding of TIMP specificity
Gillian Murphy (Cambridge, UK)
09:45 - 10:30
Mechanisms regulating protease function at the cell surface: the versatility of the plasminogen activation system
Vince Ellis (East Anglia, UK)
10:30 - 11:00 Coffee/tea break

11:00 - 11:45
Mast cell tryptases: unequal siblings
Christian Sommerhoff (Munich, Germany)
11:45 - 12:30
Substrate specificity of serine proteases
Edwin Madison (Corvas International Inc., San Diego, CA, USA)
12:30 - 14:00 Lunch

Friday afternoon

Chair:
Robin Leatherbarrow (Imperial College London, UK)
Friday 12 September 2003
12:30 - 14:00 Lunch

14:00 - 14:45
Human kallikreins: gene locus characterization and clinical applications
Eleftherios Diamandis (Toronto, Canada)
14:45 - 15:30
Signal peptidase: an atypical serine protease
Mark Lively (Winston-Salem, NC, USA)
15:30 - 16:00 Coffee/tea break

16:00 - 16:45
Protease-inhibitor docking and database searching for the inhibitor canonical loop conformation
Richard Jackson (Leeds, UK)
16:45 - 17:30
Molecular structure of human angiotensin converting enzyme (ACE)
Ravi Acharya (Bath, UK)
19:15 Boat trip and BBQ