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Experimental approaches to protein:protein interactions

11—12 January 2010

University of Sheffield, UK



Organizers:
Mike Sutcliffe (University of Manchester, UK)
Mike Williamson (University of Sheffield, UK)

Session 1

 

Chair:
Mike Williamson
Monday 11 January 2010
10:00 - 11:30 Registration with tea and coffee

11:30 - 11:40
Welcome
11:40 - 12:30
Evolution and assembly of protein complexes
Plenary lecture - Sarah Teichmann (MRC Laboratory of Molecular Biology, UK)
12:30 - 12:45 Semantic Biochemical Journal presentation

12:45 - 13:30 Lunch

Session 2

 

Chair:
Stephen Harding
Monday 11 January 2010
13:30 - 14:00
Study of protein complexes using Tandem Affinity Purification-Mass Spectrometry
Pierre-Olivier Angrand (CNRS, France)
14:00 - 14:30
Calorimetry as a tool for understanding biomolecular interactions and an aid in drug design
John Ladbury (M. D. Anderson Cancer Center, USA)
14:30 - 14:50
Molecular architecture of the stressosome, a signal integration and transduction hub
Selected oral communication - Richard Lewis (Newcastle University, UK)
14:50 - 15:10
Selectivity in a barren landscape: The P450BioI-ACP complex
Selected oral communication - Max Cryle (Max-Planck Institute for Medical Research, Germany)
15:10 - 15:40 Coffee/tea break

Session 3

 

Chair:
John Ladbury
Monday 11 January 2010
15:40 - 16:10
Protein: protein interactions of complement factor H using a multidisciplinary strategy
Steve Perkins (University College London, UK)
16:10 - 16:40
Insight into protein:protein interactions from analytical ultracentrifugation
Stephen Harding (University of Nottingham, UK)
16:40 - 17:00
Inducible protein dimerisation for signal transduction
Selected oral communication - Dominik Erhart (University of Basel, Switzerland)
17:00 - 19:00 Poster Session 1 and networking event

19:30 - 22:00 Conference dinner at Firth Hall

Session 4

 

Chair:
Paul Dupree
Tuesday 12 January 2010
09:00 - 09:50
Synchrotron Radiation Circular Dichroism (SRCD) Spectroscopy - A new method for examining protein conformations and protein:protein interactions
Bonnie Wallace - AstraZeneca Award lecture (University of London, UK)
09:50 - 10:20
Information-driven modelling of biomolecular complexes
Alexandre Bonvin (Utrecht University, The Netherlands)
10:20 - 10:40
Protein-Protein interaction using isotope-edited Fourier transform infrared spectroscopy
Selected oral communication - Parvez Haris (De Montfort University, Leicester, UK)
10:40 - 11:10 Coffee/tea break

Session 5

 

Chair:
Michael Dyson
Tuesday 12 January 2010
11:10 - 11:40
Optimising expression of mammalian proteins for interaction studies
Michael Dyson (University of Cambridge, UK)
11:40 - 12:00
Complexes of ceruloplasmin with proteins of neutrophils and their probable biochemical roles
Selected oral communication - Vadim Vasilyey (Institute for Experimental Medicine, Saint-Petersburg, Russia)
12:00 - 12:30
Probing protein interactions using single molecule fluorescence
David Klenerman (University of Cambridge, UK)
12:30 - 13:30 Lunch and Poster Session 2

Session 6

 

Chair:
Mike Sutcliffe
Tuesday 12 January 2010
13:30 - 14:00
Large-scale screening for novel low affinity extracellular protein interactions
Gavin Wright (The Wellcome Trust Sanger Institute, UK)
14:00 - 14:30
Proteomic Complex Detection using Sedimentation (ProCoDeS): A methodology for identification of proteins in complexes
Paul Dupree (University Of Cambridge, UK)
14:30 - 14:40 Presentation of the poster prize

14:40 - 15:15 General discussion and concluding remarks

15:15 Coffee/tea break and close of meeting