Pre-doctoral Meeting, 18 December 2000

University of Sussex

19 - 21 December 2000

On-line poster abstract submissions are invited for Biochemical Society Meeting 672. The poster registration deadline for this meeting is Monday 2 October. We regret that gridsheets for abstracts can no longer be accepted.

Best Poster Prize
The Biochemical Society will award £200 for the best poster displayed at the University of Sussex meeting and £50 for the runner up. The judges will consider scientific content and presentation when deciding the winner. The only criterion for being considered for the prize is that the presenter is a student member of the Biochemical Society.

Trade Exhibition

POSTER ABSTRACTS

MISCELLANEOUS

Poster Session Times: Tuesday 19 December, 17.00–18.30
Wednesday 20 December, 17.00–18.30
Thursday 21 December, 10.30–11.55

1 Kinetics of tyrosine phosphorylation in the T-cell receptor (TCR) zeta chain

H.R. Street, T. Crabbe, M.J. Perry and M.G. Gore

2 Role of tyrosine residues of Protein L in the binding reaction with human IgG

N.G. Housden, J.A. Beckingham, N.M. Muir, S.P. Bottomley and M.G. Gore

3 Effect of chronic hypoxia on the changes of reactive oxygen species and apoptosis in the pancreas

P.S. Leung, Y.W. Chan and S.P. Ip

HOST/MOLECULAR ENZYMOLOGY GROUP/REGULATION IN METABOLISM GROUP COLLOQUIUM
Fatty Acid Oxidation and Ketone Body Metabolism

Poster Session Times: Tuesday 19 December, 17.00–18.30
Wednesday 20 December, 17.00–18.30
Thursday 21 December, 10.30–11.55

4 Comparative effects of hemipalmitoyl carnitinium (HPC) stereoisomers on hepatic mitochondrial carnitine palmitoyltransferases (CPTs) in the diabetic rat

N.E. Neptune, P.S. Savle, I.U. Nam, R.D. Gandour and K. Kashfi

5 The mouse Nudt7 nudix hydrolase gene encodes a peroxisomal coenzyme A diphosphatase homologous with yeast Pcd1p

L. Gasmi and A.G. McLennan

6 Cloning and functional expression of human peroxisomal 2,4-dienoyl-CoA reductase

K. De Nys, E. Meyhi, G. Mannaerts and P. Van Veldhoven

7 Is a microsomal aldehyde dehydrogenase involved in the degradation of 3-methyl branched fatty acids?

V. Foulon, L. Van Vaeck, S. Asselberghs, G. Mannaerts, P. Van Veldhoven and M. Casteels

8 Beta-Oxidation of docosahexaenoic acid and tetracosahexaenoic acid in isolated rat hepatocytes and human fibroblasts

K. De Nys, E. Meyhi, S. Asselberghs, G. Mannaerts and P. Van Veldhoven

9 Arachidonate and docosahexaenoate could be biochemical markers to differentiate between Crohn’s disease and ulcerative colitis

D.C. Bitsanis, R. Heuschkel, K. Ghebremeskel, J. Walker-Smith and M.A. Crawford

10 Characterization of mutations in six liver-type carnitine palmitoyltransferase I (L-CPT-I)-deficient patients

N.F. Brown, R.S. Mullur, V. Esser, M.J. Bennett, J.M. Saudubray, A.S. Feigenbaum, J.A. Kobari, P.M. Macleod, J.D. McGarry and J.C. Cohen

11 Impact of the intra-mitochondrial enzyme organization on fatty acid -oxidation

H. Schulz, W. Le and X. Liang

12 Molecular analysis of hepatic carnitine palmitoyltransferase I deficiency in an extended Hutterite family

C. Prip-Buus, L. Thuillier, N. Abadi, C. Prasad, L. Dilling, F. Demaugre, C. Greenberg, S. Gobin, J. Girard and J.-P. Bonnefont

13 The NADH diphosphatase encoded by the Saccharomyces cerevisiae NPY1 nudix hydrolase gene is located in peroxisomes

S.R. AbdelRaheim, J.L. Cartwright, L. Gasmi and A.G. McLennan

14 Organization of the liver-type human carnitine palmitoyltransferase I (CPTIA) gene

S. Gobin, J.P. Bonnefont, C. Prip-Buus, C. Mugnier, F. Demaugre, M. Ferrec, J. Girard and L. Thuillier

15 Muscle cell lipid and insulin sensitivity

L.A. Baur and C.H. Thompson

16 Glutamine increases fatty acid oxidation in neonatal hepatocytes from both control and endotoxaemic rats

S.-C. Kim, M. Zamparelli, L. Spitz, A. Pierro and S. Eaton

17 Shifted co-operation of desaturation and oxidation of palmitic acid in rat liver

L. Demizieux, P. Degrace, J. Gresti, M. Narce, J.-P. Poisson and P. Clouet

18 Isoprenoid biosynthesis is not compromised in a Zellweger syndrome mouse model

I. Vanhorebeek, M. Baes and P.E. Declercq

19 Supplementation of peroxisome-deficient mice with docosahexaenoic acid and plasmalogen precursors

A. Janssen, P.E. Declercq, M. Baes and P.P. Van Veldhoven

20 Biochemical and genetic studies in a family with short-chain acyl-CoA dehydrogenase (SCAD) deficiency

P. Vreken, L.A. Bok, N. Gregersen, J.P.N. Ruiter, S. Denis, L. Ijlst, F.A. Wijburg, H.R. Waterham and R.J.A. Wanders

21 Analysis of serum pristanic and phytanic acid stereoisomers in Zellweger syndrome, Refsum’s disease, D-bifunctional protein deficiency and -methylacyl-CoA racemase deficiency

P. Vreken, H. Rusch, S. Ferdinandusse, L. van Lint and R.J.A. Wanders

22 Plasma analysis of di- and tri-hydroxycholestanoic acid (DHCA and THCA) stereoisomers using HPLC tandem-mass spectrometry, and its application in peroxisomal alpha-methylacyl-CoA racemase deficiency

S. Ferdinandusse, H. Overmars, S. Denis, H.R. Waterham, R.J.A. Wanders and P. Vreken

23 Abnormal cardiolipin and phosphatidylglycerol remodelling in Barth syndrome

P. Vreken, F. Valianpour, L.A. Grivell, L.G. Nijtmans, R.J.A. Wanders and P.G. Barth

24 Analysis of free and conjugated varanic acid diastereoisomers using HPLC electrospray tandem-mass spectrometry in patients with D-bifunctional protein deficiency

P. Vreken, H. Overmars, L. van Lint, E.G. van Grunsven, D.H. Hunneman and R.J.A. Wanders

25 System properties of ATP supply and demand in exercising skeletal muscle in vivo

G.J. Kemp, M. Roussel, D. Bendahan, Y. Le Fur and P.J. Cozzone

26 Ontogeny of peroxisomal lipid-metabolizing pathways in the mouse

S. Huyghe, M. Casteels, A. Janssen, P.E. Declercq, P.P. Van Veldhoven and M. Baes

76 Phylogenetic relationships of carnitine- and choline-acyltransferases

F.R. van der Leij, N.C.A. Huijkman, J.R.G. Kuipers and B. Bartelds

77 Mitochondrial respiration on rumenic and linoleic acids

P. Clouet, L. Demizieux, J. Gresti and P. Degrace

78 Regulation of rat heart lipid metabolism during mildronate-induced L-carnitine deficiency

P. Degrace, L. Demizieux, J. Gresti and P. Clouet

79 Influence of the N-terminal domain of rat liver-type carnitine palmitoyltransferase I (L-CPTI) on the folding state of its catalytic C-terminal domain

I. Cohen, F. Guillerault, J. Girard and C. Prip-Buus

82 Mitochondrial carnitine acylcarnitine translocase (CACT) deficiency: identification of new cases using a novel assay followed by molecular analysis of the CACT gene

L. Ijlst, J.P.N. Ruiter, W. Oostheim, H.R. Waterham, V. Lacobazzi, F. Palmieri and R.J.A. Wanders

83 Peroxisomal beta-oxidation in yeast: new insights into the mechanisms involved, with emphasis on the role of PEX11P and YPR128CP, two peroxisomal membrane proteins, in b-oxidation and peroxisomal proliferation

C.W.T. van Roermund, M. van den Berg, H.F. Tabak, R.J.A. Wanders and E.H. Hettema

84 Mutations in the gene encoding peroxisomal alpha-methylacyl-CoA racemase cause adult-onset sensory motor neuropathy

S. Ferdinandusse, S. Denis, P.T. Clayton, A. Graham, J.E. Rees, J.T. Allen, B.N. McLean, A.Y. Brown, P. Vreken, H.R. Waterham and R.J.A. Wanders

85 Azole sterol biosynthesis inhibitors as anti-mycobacterial agents

C.A. Jackson, D.C. Lamb, D.E. Kelly and S.L. Kelly

NUCLEIC ACIDS AND MOLECULAR BIOLOGY GROUP COLLOQUIUM
New Frontiers in DNA Repair

Poster Session Times: Tuesday 19 December, 17.00–18.30
Wednesday 20 December, 17.00–18.30

27 A new model of DNA binding by bacteriophage T5 exonuclease, a flap endonuclease

J.J. Dervan, L.M. Allen and J.R. Sayers

28 Mutagenesis of conserved residues in bacteriophage T5 5'3' exonuclease supports the model of separate mechanisms of endo- and exo-nucleolytic cleavage

D. Patel, S.J. Garforth, T.J. Pickering, J.A. Grasby and J.R. Sayers

29 Differential binding of Mn(II) to bacteriophage T5 5'-nuclease, and vital role of metal site II in exonucleolytic cleavage

M. Feng, I. Hag, J. Dervan, D. Patel, L. Allen and J. Sayers

30 DNA binding and hydrolysis catalysed by a flap endonuclease

J.R. Sayers, D. Patel, M. Feng and J.J. Dervan

31 SUMO-1 modification in S. pombe

J.C.Y. Ho, D.L. Taylor and F.Z. Watts

32 Processing of methylated DNA by the Escherichia coli AlkB protein

S. Dinglay, S.C. Trewick, T. Lindahl and B. Sedgwick

80 Stimulation of DNA-dependent protein kinase (DNA-PK) by radiation-induced complex single-strand breaks

P. Palmer, C.J. Skidmore and P. O’Neill

81 The green tea polyphenol, epigallocatechin 3-gallate, protects against the oxidative damage of UVA radiation

S. Tobi

BIOENERGETICS GROUP COLLOQUIUM
Protein Engineering of Peroxidases and Cytochrome P450

Poster Session Times: Tuesday 19 December, 17.00–18.30
Wednesday 20 December, 17.00–18.30

33 Blood from pre-eclamptic women and peroxidized normotensive blood contain an extractable vasoactive factor

E.H. Sharp, N.F. Thomson, J.F. Clark, D.A. Harris and S. Thornton

34 Haloperoxidase activity of bacterial acid phosphatases

N. Tanaka, V. Dumay and R. Wever

35 NMR investigations into the binding of azo dyes to horseradish peroxidase

J.J.F. Coen, A.T. Smith and N.C. Veitch

36 Efficient hydroxylation catalysts based upon P450 enzymes derived from Actinomycetes

H.A. Hussain and J.M. Ward

37 Cytochromes P450 as drug targets in Mycobacterium tuberculosis

K.J. McLean and A.W. Munro

38 Structure–function analysis of lignin peroxidase

W.A. Doyle, W. Blodig, K. Piontek and A.T. Smith

39 Lanosterol demethylase P450 in Mycobacterium tuberculosis

A. Souter, K.J. McLean and A.W. Munro

40 Reconstituting the electron-transport system in cytochrome P450 BioI

A.J. Green, A.W. Munro, S.L. Rivers, G.A. Reid and S.K. Chapman

41 Signal-sequence-appended native and soluble cytochromes P450 can be targeted to the periplasm of Escherichia coli for enhanced biocatalysis in vivo

C.C. Ugochukwu, M. Kaderbhai, S.L. Kelly and D.C. Lamb

42 Investigation of the active site of ascorbate peroxidase

M. Mewies, L. Lad and E.L. Raven

43 Comparative analysis of substrate-access-channel dynamics in three bacterial cytochrome P450s reveals structurally similar pathways, but different opening mechanisms

P.J. Winn, S.K. Lödemann, R. Gauges, V. Lounnas and R.C. Wade

44 Characterization of the horseradish peroxidase (HRP) distal pocket mutants Arg³⁸His/His⁴²Gln, Arg³⁸His/Phe⁴¹Ala/His⁴²Gln and Arg³⁸Ala/Phe⁴¹His/His⁴²Gln

F. Schneider-Belhaddad and A.T. Smith

45 Reaction of ascorbate peroxidase with hydrogen peroxide: evidence for a protein radical

A.N.P. Hiner, E. Raven, M.B. Arnao, M. Acosta, J.I. Martínez and J.N. Rodríguez López

46 Escherichia coli expression and in vitro folding optimization of Pleurotus eryngii peroxidase

M. Perez-Boada, W.A. Doyle, F.J. Ruiz-Dueñas, M.J. Martinez, A.T. Martinez and A.T. Smith

48 T-2 toxin induces apoptosis in normal human lymphocytes

T. Moodley, A.A. Chuturgoon, M.F. Dutton, C. Branford-White and M.A. Crawford

49 A proposed role for serine hydroxymethyltransferase in the control of the thymidylate cycle

K. Snell

50 Inactivation of ascorbate peroxidase and horseradish peroxidases A2 and C by hydrogen peroxide

A.N.P. Hiner, J. Hernández Ruíz, M.B. Arnao, F. García Cánovas and M. Acosta

51 A structural determinant of phenolic substrate oxidation in horseradish peroxidase C

G.A. Williams, N.C. Brissett, M. Gajhede and A.T. Smith

52 Azole resistance mechanisms in clinical isolates of Candida albicans and Cryptococcus neoformans from intensive-care patients

D. Greetham, D. Lamb, D. Kelly and S. Kelly

53 Phenylalanine-393: controlling the electronic properties of the haem in flavocytochrome P450 BM3

T.W.B. Ost, C.S. Miles, A.W. Munro, G.A. Reid and S.K. Chapman

54 Kinetic characterization of the catalase–peroxidase enzyme from Mycobacterium tuberculosis

N.A.J. Eady, J.M. Nagy, A.E.G. Cass and K.A. Brown

55 Probing the stability of the class II peroxidase from Coprinus cinereus

N.C. Brissett, L. Jørgensen, P. Schneider and A.T. Smith

56 Site-directed mutants of the active-site residues of catalase–peroxidase from Mycobacterium tuberculosis

Jesmin, J.M. Nagy, A.E.G. Cass and K.A. Brown

57 Engineering a soluble, catalytically self-sufficient human P450 for nanobiotechnology

S. Giannini, M. Fairhead and G. Gilardi

58 Redox properties of human cytochrome P450 reductase

A.W. Munro, M.A. Noble, S.N. Daff and S.K. Chapman

SOCIETY SPECIAL COLLOQUIUM
Role of Specific Cytokines and Cytokine Receptors in Fetal Growth

Poster Session Times: Wednesday 20 December, 17.00–18.30
Thursday 21 December, 10.30–11.55

59 Molecular evolution of growth hormone in primates

O.C. Wallis and M. Wallis

60 Detection of polymorphisms in normally grown and growth-retarded infants

L. Forsyth, R. Hume, A. Howatson and A. Burchell

61 Cortisol has no effect on prolactin receptor abundance in brown adipose tissue in late-gestation fetal sheep

S. Pearce, H. Budge, A. Forhead, A. Fowden, P. Ingleton, M.E. Symonds and T. Stephenson

62 Molecular evolution of growth hormone in Cetartiodactyla

Z. Maniou, O.C. Wallis and M. Wallis

63 Effect of chronic leptin administration on plasma leptin, prolactin and uncoupling protein-1 in neonatal lambs

A. Mostyn, S. Pearce, H. Budge, C. Morrison, D. Keisler, R. Webb, T. Stephenson and M.E. Symonds

64 Variable longitudinal patterns of leptin expression during human pregnancy

K. Stafford, V. Jha, A.J. Harrold and A. Burchell

66 An in vitro system for the study of cytokine activity

F. Imtiaz, G. Barden, C. Underwood, B. Fuller and M.C. Winslet

67 Fucoidan interacts with transforming growth factor- and modulates its activity: implications for adult wound repair by mimicking the fetal environment

R. O’Leary, M. Rerek and E.J. Wood

HORMONE GROUP COLLOQUIUM
Steroids, Steroid Receptors and Disease

Poster Session Time: Tuesday 19 December, 17.00–18.30

68 Development of a mammalian screen for the detection of compounds with androgenic activity

J.R. Higman, P.D. Darbre, G.S. Pope and M.J. Sauer

69 Isobutylparaben is oestrogenic to human breast-cancer cells

P.D. Darbre, J.R. Higman, G.S. Pope, R.A. Horton and M.J. Sauer

70 3'-Azido-2',3'-dideoxythymidine (AZT)-sterol dicarboxylates and sterol-PFA bioesters as novel HIV-provirus inhibitors

J.M. Bailey

71 Parabens bind directly to recombinant human oestrogen receptor ligand-binding domain

M.F. Byford, J.R. Higman, P.D. Darbre and M.J. Sauer

GLYCOBIOLOGY GROUP/BRITISH SOCIETY FOR DEVELOPMENTAL BIOLOGY
The Glycobiology of Development

Poster Session Times: Wednesday 20 December, 17.00–18.30
Thursday 21 December, 10.30–11.55

72 Molecular analysis of the glucose-6-phosphatase enzyme system in a family with glycogen storage disease type Ib

K.J. Isaac and A. Burchell

74 Heparan sulphate in the regulation of basic fibroblast growth factor

S.J. Goodger, D.A. Pye and J.T. Gallagher

75 Glycosylation and Notch signalling

E. Selva, U. Haecker, K. Hong, S.J. Turco and N. Perrimon

LATE ABSTRACTS

86 Heterologous CYP51 expression to probe altered interactions with azole inhibitors

T. Marczylo, D.C. Lamb, D.E. Kelly and S.L. Kelly

87 Probing the activity of agrochemicals against plant and fungal CYP51

M. Cannieux, D.C. Lamb, D.E. Kelly, R. Kahn, S. Bak and S.L. Kelly

88 Manipulation of P450 in Acinetobacter and application to pollutant degradation

S.L. Kelly, D.E. Kelly, G. Jones and D.C. Lamb

89 Heterologous expression of Streptomyces griseus CYP105D1: comparative production methods, diverse xenobiotic substrate range, activation effects and biological function

H. Song, D.C. Lamb, M. Taylor, M. Dawson, R. Cannell, D.B. Kell and S.L. Kelly

90 Yeast cytochrome P450 reductase: expression of a soluble and full-length form, kinetic mechanisms and crystallization

D.C. Lamb, A. Warrilow, K. Venkateswarlu, D.E. Kelly and S.L. Kelly

Biochemical Society/Portland Press Third Floor Eagle House 16 Procter Street London WC1V 6NX United Kingdom Map Biochemical Society Registered Company Number 892796 limited by guarantee VAT No. GB 523 2392 69 Registered Charity Number 253894 Portland Press Ltd Registered Company Number 2453983 Vat No GB 523 2392 69 Biochemical Society Membership Office Portland Customer Services Commerce Way Whitehall Industrial Estate Colchester Essex CO2 8HP United Kingdom Map

Administration: Tel: 020 7280-4100 - Fax: 020 7280-4170 E-mail: genadmin@biochemistry.org Editorial: Tel: 020 7280-4110 - Fax: 020 7280-4169 E-mail: editorial@portlandpress.com Meetings: Tel: 020 7280-4150 - Fax: 020 7280-4167 E-mail: meetings@biochemistry.org Membership: Tel: 01206 796 351 - Fax: 01206 799 331 E-mail: Membership@Biochemistry.org Customer services: Tel: 01206 796 351 - Fax: 01206 799 331 E-mail: sales@portland-services.com Read our Privacy Policy