Molecular Communications

University of Bristol

10 - 12 April 2001

Poster Abstract deadline: 15 January 2001
Registration deadline: 26 February 2001

POSTER ABSTRACTS

MISCELLANEOUS

Poster Session Times:

Tuesday 10 April, 16.00–18.00

Wednesday 11 April, 16.00–18.00

Thursday 12 April, 13.00–14.40

1 Interactive tutorials in bioenergetics

P.A.C. Paul and P.M. Wood

BIOENERGETICS GROUP COLLOQUIUM
Assembly and Repair of Membrane-Bound Electron Transport Complexes: Impact on Plant Physiology and Medicine

Poster Session Times

Tuesday 10 April, 16.00–18.00

Wednesday 11 April, 16.00–18.00

Thursday 12 April, 13.00–14.40

2 Abnormal gene expression and assembly of the multimeric complex I in the dum24 deletion mitochondrial mutant of Chlamydomonas reinhardtii

C. Remacle, F. Duby, P. Cardol and R.F. Matagne

3 Chlamydomonas reinhardtii mitochondrial mutants lacking complex I activity: characterization of the mutations and assembly of the multimeric complex I

P. Cardol, R.F. Matagne and C. Remacle

4 Study of a point mutation in the mitochondrially encoded subunit III of cytochrome oxidase affecting the complex assembly in yeast

A. Kapazoglou and B. Meunier

5 Function and regulation of fungal alternative oxidase

T. Joseph-Horne, C. Reeve, A. Hall and P.M. Wood

6 Biogenesis of the eukaryotic photosystem I complex

K. Ali and S. Purton

7 Var2 mutation in Arabidopsis affects D1 degradation

S. Bailey, E.P. Thompson, C.W. Mullineaux, P. Nixon, C. Robinson and N.H. Mann

8 Identification of a protease involved in the repair of photosystem II in Synechocystis 6803

P. Silva, S. Bailey, C.W. Mullineaux, C. Robinson, N.H. Mann and P.J. Nixon

REGULATION IN METABOLISM GROUP COLLOQUIUM
Insulin Action

Poster Session Times:

Tuesday 10 April, 16.00–18.00

Wednesday 11 April, 16.00–18.00

Thursday 12 April, 13.00–14.40

9 Role of the actin cytoskeleton in the insulin regulation of glycogen synthesis in human muscle cells

J.L. Armstrong and S.J. Yeaman

10 Different roles and regulation of insulin receptor substrate-1 and insulin receptor substrate-3 in insulin signalling in primary adipocytes

I. Hers, C.J. Bell, J.M. Tavaré and R.M. Denton

11 Modulation of signal transduction and activators of transcription (STAT)3 gene transcription through the phosphoinositide 3-kinase/Akt pathway: interaction between the transcription factors FKHR (forkhead in rhabdomyosarcoma) and STAT3

A. Barthel, M. Kortylewski, K.-D. Krueger, H. Axer, J. Behrmann, F. Feld, R.A. Roth, P.C. Heinrich and H.-G. Joost

12 A dual role for mitogen-activated protein kinase in insulin-dependent regulation of Fra-1 transcription and phosphorylation

T.W. Hurd, A.A. Culbert, J.B. Uney and J.M. Tavaré

13 Targeting protein kinase B to GLUT4 vesicles controls the sensitivity of GLUT4/insulin-regulated aminopeptidase (IRAP) translocation to insulin

P.-H. Ducluzeau, L.M. Fletcher, G.I. Welsh and J.M. Tavaré

14 Insulin-mimetic signalling by redistribution of caveolar components in rat adipocytes

G. Mueller, S. Welte, C. Jung and W. Frick

15 Inhibition of insulin receptor tyrosine kinase activity by the molecular adapter Grb14

V. Béréziat, A. Kasus-Jacobi, D. Perdereau, J. Girard and A.-F. Burnol

16 Regulation of glucose-6-phosphatase gene expression by protein kinase B and the forkhead transcription factor FKHR

D. Schmoll, R. Grempler, T.G. Unterman and R. Walther

17 Regulation of pyruvate dehydrogenase in human muscle cells

E.L. Abbot, J.G. McCormack and S.J. Yeaman

18 Hepatic glucose handling is programmed in fetal life

H.C. Murphy, R.A. Iles, R.D. Cohen and S.P. Burns

19 Microtubule integrity is not required for insulin-stimulated GLUT4 translocation in 3T3-L1 adipocytes

J.C. Molero, J.P. Whitehead and D.E. James

20 Skeletal muscle of the stroke-prone spontaneously hypertensive rat (SHRSP) is insulin- insensitive

F. Cairns, D.J. James, D. Graham, I.P. Salt, G.J. Murphy, A.F. Dominiczak, J.M.C. Connell and G.W. Gould

21 Insulin-stimulated nitric oxide production in human aortic endothelial cells

V.A. Nicolson, I.P. Salt, J.M.C. Connell, J.R. Petrie and G.W. Gould

22 Investigation into the association of insulin receptor substrate-1 with the cytoskeleton

J. Whitehead, J.C. Molero, S. Clark, S. Martin, G. Ramm and D.E. James

23 Regulation of protein kinase B activity by -adrenergic agonists in rat epididymal fat cells

C.J. Bell and S.K. Moule

24 Characterization of a PTEN homologue from Drosophila

G. McConnachie, I. Pass, N. Leslie and C.P. Downes

25 Sterol regulatory element binding protein-1c (SREBP-1c) is strongly expressed in MIN6 cells

C. Andreolas, C. Zhao, G. da Silva Xavier, A. Varadi, F. Diraison, F. Foufelle, P. Ferré and G.A. Rutter

PROTEIN AND PEPTIDE SCIENCE GROUP COLLOQUIUM
Membrane Active Peptides

Poster Session Times:

Tuesday 10 April, 16.00–18.00

Wednesday 11 April, 16.00–18.00

Thursday 12 April, 13.00–14.40

26 Nanoscale behaviour of ions in narrow channels

W.L. Duax, B.M. Burkhart and V. Pletnev

27 Pro-matrix metalloproteinase-3 (pro-MMP-3) and atherosclerotic carotid artery plaques

A.D. Khattab, I.S. Ali, R.R. Dils, D. Kerr, D. Jenkinson, S. Allen and M.Z. Rana

28 Membrane active peptides as gene-delivery agents

R.O. Phillips, J.H. Welsh, R. Husain, D. Williams, J. Charlesworth and A.M.R. Haines

29 Effect of peptide main-chain length on the membrane-modifying properties of short analogues of the lipopeptaibol antibiotic trichogin GA IV

F. Formaggio, C. Peggion, M. Crisma and C. Toniolos

30 Structure and function of equinatoxin, a novel citolysin from sea anemone

G. Anderluh, A. Barlic, P. Malovrh, Z. Podlesek, A. Athanasiadis, D. Turk, G. Viero, M. Dalla Serra, G. Menestrina and P. Macek

31 Conformation and membrane activity of analogues of the peptaibol antibiotic trichogin GA IV with a lipophilic amino acid at different positions

F. Formaggio, C. Peggion, M. Crisma, Q.B. Broxterman, B. Kaptein and C. Toniolo

32 Incorporation of a membrane-active peptide in S-layer ultrafiltration membrane-supported lipid membranes

B. Schuster, P.C. Gufler, D. Pum and U.B. Sleytr

33 Studies on the membrane anchoring of Escherichia coli penicillin-binding protein 5

D.A. Phoenix and F. Harris

34 A neutron diffraction study of lipid sensing by the membrane-binding domain of phosphocholine cytidylyltransferase

S.M.A. Davies, T.A. Harroun, M.J.M. Darkes, T. Hauss, R.B. Cornell and J.P. Bradshaw

35 Disulphide-dimerized analogues of melittin and magainin: pore kinetics and molecular dynamics simulations in membranes

C.E. Dempsey, R.B. Sessions, K. Hristova and S.H. White

36 TMAlpha: a software tool for analysis of transmembrane region -helices

K.S. Sarakinou, J.F. Antoniw, R.C. Togawa and J.G.L. Mullins

37 Oblique orientated -helix formation in the C-terminal regions of the Escherichia coli low-molecular-mass penicillin-binding proteins

F. Harris, J. Wallace and D.A. Phoenix

38 Interaction of viscotoxin A3 with model membranes of acidic phospholipids

A. Coulon, A.M. Sautereau, K. Urech, P. Rouge and A. Lopez

39 Can the loss of an exon reverse the membrane topology of a glutamate transporter?

J. Huggett and D. Mason

HOST COLLOQUIUM
Molecular Communications

Poster Session Times:

Tuesday 10 April, 16.00–18.00

Wednesday 11 April, 16.00–18.00

Thursday 12 April, 13.00–14.40

40 Transcription of variant surface glycoprotein (VSG) genes during the metacyclic development of African trypanosomes in the tsetse fly

M.L. Ginger, P. Blundell, A.M. Lewis and J.D. Barry

41 Association of small G-protein ADP ribosylation factor (ARF1) with the third intracellular loop of the M3 muscarinic receptor

D.N. Robertson, M.S. Johnson, P.J. Holland and R. Mitchell

42 Co-ordination of actin-based membrane rearrangements by a novel Rho GTPase, Rif

S. Ellis and H. Mellor

43 Study of mitogenic/cytotoxic effects of venom proteins from Scorpion fish S. verrucosa and H. rubripinnis, on normal and tumour cell lines using flow cytometry

A. Siddiq, H. Nakagawa, Al-Sofyani, K. Kanemaru, F. Satoh and Z.H. Zaidi

44 Inhibition of the phosphoinositide 3-kinase pathway sensitizes carcinoma cells to CD40-mediated apoptosis

C.C. Davies, L.S. Young and A.G. Eliopoulos

45 Interactions between phospholipase D1 and its effectors and substrate detected by fluorescence lifetime imaging microscopy (FLIM)

B. Larijani, W.H. Hughes, T. Ng and P.J. Parker

46 Specificity determinants in high-affinity complexes

A.H. Keeble, W. Li, A.M. Hemmings, G.R. Moore, R. James and C. Kleanthous

47 High-affinity binding of extracellular-signal-regulated kinase (ERK)2 to docking regions within the C-terminus of cAMP-specific phosphodiesterase family PDE4D3 is required for phosphorylation and regulation

G.S. Baillie, S.J. Mackenzie, I. McPhee, G. Bolger and M.D. Houslay

48 Molecular communication across a membrane: spatial and temporal regulation of endoplasmic reticulum (ER)-to-Golgi transport

D.J. Stephens and R. Pepperkok

49 Cloning and characterization of novel phosphoinositide effector proteins

S. Dowler, R.A. Currie, D.G. Campbell, M. Deak, G. Kular, C.P. Downes and D.R. Alessi

50 The cAMP phosphodiesterase PDE4A5 is cleaved by caspase-3 downstream of its SH3-binding domain

E. Huston, M. Beard, G. Scotland and M.D. Houslay

51 Tethered bilayers for study in membrane biochemistry

M.J. Spencelayh, P.F. Knowles and S.D. Evans

52 Confirmation of the interaction between the monocarboxylate transporter MCT1 and its ancillary protein CD147 using fluorescence resonance energy transfer

M.C. Wilson, D. Meredith and A.P. Halestrap

53 The genomic structure of the human lactate transporters MCT1 and MCT2 and evidence for alternative promoters

A.J. Davies, M.C. Wilson and A.P. Halestrap

54 Identification of critical cysteine residues whose oxidative cross-linking regulates the mitochondrial permeability transition pore

G.P. McStay, S.J. Clarke and A.P. Halestrap

55 Mitogen-activated-protein-kinase-selective regulation of pro-inflammatory cytokines in the alveolar epithelium

J.J. Haddad and S.C. Land

56 Interactions of the novel PtdIns(3,4)P2-binding proteins TAPP1 (tandem PH-domain-containing protein 1) and TAPP2 with PDZ-domain-containing proteins

W.A. Kimber, M. Deak and D.R. Alessi

57 Comparison of the efficiency of different techniques for viroid detection from citrus culture

A. Elleuch, H. Fakhfakh, L. Jendoubi, N. Bessais and M. Marrkchi

58 Cross-talk between tyrosine kinase Btk and serine/threonine kinase protein kinase C in platelets: functional role of reciprocal phosphorylation

D. Crosby and A.W. Poole

59 Possible role a tetrose-PO4 cycle in the origin of homochirality

J.M. Bailey, C.M. Fedo and G.A. Walker

60 Phospholipase D localization and substrate specificity

M. McDermott, T.R. Pettitt and M.J.O. Wakelam

61 Expression of wild-type and truncated Hermansky–Pudlak syndrome protein

J.E. Pople and B.J. Reaves

62 14-3-3 docking to the M3 muscarinic receptor

D.N. Robertson, J.K. Beggs, D.M. Collins, M.S. Johnson, P.J. Holland, E.M. Lutz, T. Dubois, A. Aitken and R. Mitchell

63 New epitope-tagged constructs of the human 5-hydroxytryptamine (HT)2A receptor

M.S. Johnson, E.M. Lutz, P.J. Holland and R. Mitchell

64 Analysis of the importance of two pleckstrin homology (PH) domains of centaurin-1 for PTDIns (3,4,5) P3 binding

K.G. Brandom and K. Venkateswarlu

MEMBRANE GROUP AND NEUROSCIENCE GROUP COLLOQUIUM
The Interface of Receptor Signalling and Trafficking

Poster Session Times:

Tuesday 10 April, 16.00–18.00

Wednesday 11 April, 16.00–18.00

Thursday 12 April, 13.00–14.40

65 Intracellular distribution of angiotensin II type-1 receptors

S.L.R. Ball, G.F. Wilkinson and J.A. Koenig

66 The Sec1p-like/Munc18 (SM) protein Vps45p acts as a t-SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) chaperone and positively regulates SNARE complex formation

N.J. Bryant and D.E. James

67 Use of a 2xFYVE–EGFP fusion protein to localize phosphatidylinositol 3-phosphate in living cells during Salmonella infection

K. Pattni, M. Jepson, H. Stenmark and G. Banting

68 Phospholipase D1b is activated by Rac1, ADP-ribosylation factor (ARF)6 and protein kinase C in RBL-2H3 cells

D.J. Powner, M. Hodgkin and M.J.O. Wakelam

69 Regulation of epidermal growth factor receptor traffic by RhoB

A. Gampel, M. Wherlock and H. Mellor

70 Casein kinase II is associated with clathrin-coated vesicles

V.I. Korolchuk and G. Banting

71 Tyrphostins: inhibitors of membrane traffic pathways?

D. Banbury, J. Oakley, C. Crump, R. Sessions and G. Banting

72 Agonist-induced internalization of the mGlu1a receptor is arrestin- and dynamin-dependent

S.J. Mundell, A.L. Matharu, G. Pula, P.J. Roberts and E. Kelly

73 Effect of pertussis toxin on the internalization of the somatostatin2 receptor

L.A. Frawley and J.A. Koenig

74 Dual regulation of endothelial differentiation gene (EDG)1 receptor phosphorylation and internalization by sphingosine-1-phosphate and protein kinase C is controlled by its C-terminus

K.R. Watterson and T.M. Palmer

75 Internalization of mammalian and non-mammalian GnRH receptors: uncoupling of dynamin-dependent internalization from mitogen-activated protein (MAP) kinase signalling

J.N. Hislop, H.M. Everest, A. Flynn and C.A. McArdle

76 Purification of a novel 50kDa protein that regulates Ca2+-dependent exocytosis in mast cells

G.A. Way and A.J. O’Sullivan

77 Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) regulates endosomal trafficking and tyrosine kinase receptor signalling

T.E. Lloyd, R.A. Atkinson, M.N. Wu and H.J. Bellen

78 Study of a novel protein CHC22, a muscle-specific form of clathrin

M.C. Towler, S.-H. Lui, E. Chen, C.-Y. Chen, W. Song, G. Apodoca and F.M. Brodsky

79 Regulation of endogenous secretin-receptor responsiveness by protein kinase C

R. Ghadessy and E. Kelly

80 Localization of presynaptic metabotropic glutamate receptors at the Calyces of Held

B. Pilkington, M. Cuttle, J. Chad and V. O’Connor

81 A role for receptor internalization in M3 muscarinic receptor association with ADP-ribosylation factor (ARF) and activation of phospholipase D

D.N. Robertson, M.S. Johnson, E.M. Lutz, P.J. Holland and R. Mitchell

82 In vivo imaging of ryanodine-receptor-mediated release of Ca2+ from dense core secretory vesicles

K. Mitchell, A. Varadi, P. Pinton, T. Pozzan, R. Rizzuto and G.A. Rutter

83 Desensitization and trafficking of the rat A2B adenosine receptor

A. Matharu, S. Mundell and E. Kelly

84 An investigation of the role of TGN38 in secretion and characterization of its lumenal domain

S.S. Lee and G. Banting

85 Azithromycin (AZ), a lysosomotropic antibiotic, differently impairs various types of endocytosis in cultured macrophages

D. Tyteca, F. Van Bambeke, P.M. Tulkens, P.J. Courtoy and M.P. Mingeot-Leclercq

86 Investigation of surface expression of a GluR2 -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunit tagged with a pH-sensitive green fluorescent protein

S.A. De La Rue, C.J. Matthews, G.S. Ralph, J.B. Uney, G.L. Collingridge and J.M. Henley

87 Role of phosphatidylinositol 3-kinases in sorting of epidermal growth factor receptors within multivesicular endosomes

C.E. Futter, L.M. Collinson, J.M. Backer and C.R. Hopkins

88 Mutations in the band 3 (AE1) gene associated with distal renal tubular acidosis

A.M. Toye, L.J. Bruce, O. Wrong, M.T. Young, R.J. Unwin and M.J.A. Tanner

89 Activation of -aminobutyric acid (GABA)-B receptors causes a translocation of activating transcription factor 4 (ATF4) from the nucleus in cultured hippocampal neurons

L.A. Pickard, G. Meyer, E. Molnar and J.M. Henley

90 Modulation of recombinant NR1a/NR2A receptors by mGluR5a activation in HEK-293 cells may involve tyrosine phosphorylation

V.J. Collett and G.L. Collingridge

91 Characterization of the interaction between the kainate receptor subunit GluR5-2b and PICK1 (protein interacting with C-kinase)

J.C. Francis, H. Hirbec, S.P. Braithwaite, K. Dev and J.M. Henley

92 Developmental profile of -aminobutyric acid (GABA)-B receptor subunit expression in hippocampal cultures

R.J. Sutch, L. Pickard and J. Henley

93 Tethering proteins in endoplasmic reticulum (ER)–Golgi trafficking

C.I. Alvarez and E.S. Sztul

94 Syntenin interacts with kainate receptors: in vitro and in vivo evidence

H. Hirbec, S.E. Lauri, J.C. Francis, S.P. Braithwaite, J.T. Isaac, G.L. Collingridge and J.M. Henley

95 Use of glutathione S-transferase pull-down assays to identify new interactions between the PICK1 (protein interacting with protein C-kinase) and GRIP proteins and the glutamate receptors

O. Perestenko, H. Hirbec, K. Dev, A. Nishimune, G. Meyer, J.C. Francis and J.M. Henley

96 -Aminobutyric acid (GABA)-B receptor binds a novel scaffolding protein that forms a multiple signal transduction protein complex

A. Nishimune, S. Kantamneni, N. Vinh, G. Meyer and J.M. Henley

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