Biochemical Society Meeting 678
Biochemical Aspects of Health and Disease

Imperial College, London

16 - 18 December 2002

Research Colloquium: Biological Functions of Sulphated Glycoproteins
Tuesday 17 December at 9.15

Registration for the meeting is in the Sherfield Building
From South Kensington underground (Exhibition Road exit), follow Exhibition Road to Imperial College Road. Follow Imperial College Road to Queen's Lawn, Sherfield Building is on the right across Queen's Lawn.
Imperial College London South Kensington campus map

POSTER ABSTRACTS

Abstracts first published on the Internet 13 November 2002

Proteases and the Regulation of Biological Processes
Annual Symposium

Poster Session Times:

1-17: Tuesday 17 December 14:50-16:20

18-34: Wednesday 18 December 10:20-11:50

1 Investigation of the proteolytic activity of recombinant human ADAMTS-1, -4 and -5.

M.N. Vankemmelbeke, G.C. Jones and D.J. Buttle

2 Proteolytic activity in human amnion: a comparison of normal deliveries and those complicated by premature rupture of membranes

E. Stuart, H. Powers and G. Evans

3 Membrane-linked 15-lipoxygenase activation and inhibition of Abeta42 amyloidogenic peptide formation by ibuprofen: possible link to supression of Alzheimer's disease.

J.M. Bailey and J.Y. Vanderhoek

4 Development of a broadly applicable surface plasmon resonance-based proteolytic assay

S.S. Taremi and W.W. Prosise

5 Regulation of epithelial cell interactions and migration by ADAM 17

A.J. Messent, D.V. Box, R.A. Maciewicz and M.J. Humphries

6 Molecular cloning and biochemical characterization of bovine lung tryptase

L. Fiorucci, A. Gambacurta, P. Basili, F. Erba, A. Amoresano and F. Ascoli

7 Differential regulation of inositol 1,4,5-trisphosphate by protease activated receptors on KU812 basophilic cells.

J.R. Purkiss, A. Mochizuki and A.F. Walls

8 Regulation of insulin secretion by calpain-10: implications for type 2 diabetes

C. Marshall, G.A. Hitman and M.D. Turner

9 Poster Withdrawn

10 The LIV-1 subfamily of ZIP transporters have a unique metalloprotease motif, HEXPHEXGD.

K.M. Taylor and R.I. Nicholson

11 Targeting angiotensin-converting enzyme to lipid rafts via a GPI anchor does not affect its protein kinase C-mediated shedding

E.T. Parkin, A.J. Turner and N.M. Hooper

12 Expression and stimulation of protease-activated receptors on malignant cancer cell lines

S.R. Wilson, S.A. Gallagher, B. Walker, K. Warpeha and S.J. Hawthorne

13 Oxidative stress induces intracellular relocation of the thromboxane receptor in Cos-cells

F. Valentin, M.C. Field and J.R. Tippins

14 Maspin inhibits cell migration in the absence of protease inhibitory acitivity

R. Bass, A.-M.M. Fernandez and V. Ellis

15 Ceramide activates ProMMP-2 in articular cartilage by a novel mechanism involving the protein kinase, PKR.

S.J. Gilbert, V.C. Duance and D.J. Mason

16 Receptor-mediated IL-6 production in prostate cancer cells

S. Gallagher, S. Wilson, B. Walker and S. Hawthorne

17 Tryptase in human basophils: quantitation, secretion, and sub-cellular localisation

A. Mochizuki, N.J.W. Easom, M.G. Buckley, A.F. Walls and A.R. McEuen

18 Thimet oligopeptidase : development of novel tailored antibodies from a human synthetic scFv phagemid library

L. Morrison J. Conner and A.R. Pierotti

19 Investigating interactions between the hepatocyte growth factor/scatter factor and plasminogen activation systems

K.A. Owen and V. Ellis

20 Matriptase is processed at Gly149 in a conserved "SEA" module

L.M. Kilpatrick and V. Ellis

21 Functional characterisation of the acidic domain of NRD-convertase.

K. Singleton and A.R. Pierotti

22 Different expression pattern of matrilysin by Epstein-Barr virus-immortalized B lymphocytes from healthy donors and chronic granulomatous disease patients

C. Trocme, L. Grange, M.J. Stasia, P. Gaudin and F. Morel

23 Transcriptional regulation of the thimet oligopeptidase gene.

L. Morrison and A.R. Pierotti

24 Substrate-derived inhibitors of sortase, a bacterial cysteine protease-transpeptidase

B. Walker, C.J. Scott, A. McDowell, S.L. Martin and K. Vandenbroeck

25 The effect of deamidation on antigen processing

C.X. Moss and C. Watts

26 Characterization using surface plasmon resonance (SPR) and enzyme kinetics of the interaction of a synthetic peptide corresponding to the carboxy-terminus of the leech inhibitor Antistasin (Arg109-Ser119) with human coagulation Factor Xa [FXa].

M.T. Fox, B. Greer, G.J. Allen, J. Lawson, A. Healy and P. Harriott

27 The expression of trypsin in cells expressing protease-activated receptor 2

G.S. Cottrell, S. Amadesi, E.F. Grady and N.W. Bunnett

28 Effect of complement, cytokines and growth factors on the expression of MMP-2 and MMP-9 in porcine aortic smooth muscle cells

S. Capey and C.W. van den Berg

29 Subcellular localisation of caspase-9 in somatotrophs

M. Potokar, I. Milisav, M. Kreft, M. Stenovec and R. Zorec

30 Insights into the regulatory mechanism for caspase-8 activation

M. Donepudi, A. Mac Sweeney and M.G. Gruetter

31 Characterisation of SSP3, a nuclear SUMO specific protease

H. Mendoza, E. Jaffray, G.D. Kemp, L.-N. Shen, M.H. Tatham, O.A. Vaughan D. Chotai, A. Lewis, B. Ink and R.T. Hay

32 Timp-1 gene regulation by acetylation

D.A. Young, D.R. Edwards and I.M. Clark

33 Characterisation of matrix metalloproteinase homologues in Arabidopsis thaliana

M.D. Thomas, S. de Vos and I.M. Clark

34 Design of inhibitors for prostate-specific antigen using combinatorial library

C.F. Yang and Z. Cai

 

Late breaking abstracts:

120 Effects of omega-3 fatty acids on the expression of metalloproteinases in monocytic cell lines

J. Worley, X. Scott, D.A. Hughes, M.J. Sampson and J. Gavrilovic

121 Protein kinase C-?,?1,?2 expression in rat-liver treated with phenobarbital or WY-14,643

P. Pantasri, R. Rapley, R. Hoffman, P.G. Lord, M.J. Skehel, K. Kramer and K.M. Doyle

122 The TIMP-2 tail : its role in proMMP-2 activation by MT1-MMP

V. Knäuper, J.R. Worley, P. Soloway and G. Murphy

123 Biochemical basis for connective tissue laxity around parturition

L. Knott, A.J. Webster and J.F. Tarlton

124 TIMP-3 inhibits aggrecanase-mediated GAG release in IL-1?-stimulated bovine cartilage explants

C. Gendron, M. Kashiwagi, C. Hughes, B. Caterson and H. Nagase

125 Overexpression of the hemopexin domain (PEX) of MT1-MMP inhibits pericellular collagenolytic activity of MT1-MMP

Y. Itoh, H. Nagase, and M. Seiki

Polyamines and their Role in Human Disease

Poster Session Times:

35-43: Monday 16 December 14:50-16:20

44-51: Tuesday 17 December 14:50-16:20

35 Inhibition of the activity of the brain plasma membrane Ca2+-ATPase by spermine

J. Palacios, M.R. Sepúlveda and A.M. Mata

36 Gene expression analysis of mouse polyamine oxidase (PAOm)

M. Cervelli, A. Bellini, F. Polticelli, R. Federico and P. Mariottini

37 Bcl-2 overexpression prevents the cytotoxic effects of the unsymmetrically substituted polyamine analogues in human leukaemic cells

A.V. Fraser, C.M. Stefanelli, P.M. Woster and H.M. Wallace

38 The relation between proliferation, apoptosis and mitochondrial respiration in human breast cancer cell lines treated with N1,N11-diethylnorspermine

M. Holst and S.M. Oredsson

39 Defective cell cycle progression in MCF-7 breast cancer cells treated with the spermine analogue N1,N11-diethylnorspermine

L. Myhre, P. Berntsson and S. Oredsson

40 Increased delay in cell cycle progression by treatment with 2-difluoromethylornithine or N1N11-diethylnorspermine in a mouse cell line lacking spermine synthase

K. Alm, C.A. Macintosh, A.E. Pegg and S.M. Oredsson

41 Mammalian putrescine biosynthesis

C.S. Coleman and A.E. Pegg

42 CYP1A1 genotype and colorectal cancer

N.T. Brockton, J. Cassidy, N. Haites and J. Little

43 Polyamine depletion by non-steroidal anti-inflammatory drugs in human bladder cancer cells

A. Hughes, A. Milsom, L. Crow, R.A. Lockhart, A. Alhasso, J. N'Dow and H.M. Wallace

44 Rapid turnover of Crithidia fasciculata ornithine decarboxylase

S. Nasizadeh and L. Persson

45 Altered polyamine synthesis and NMDA receptor expression during experimental allergic encephalomyelitis (EAE)

E.R. Wheeler, E.M. Walemba, C. Bolton, N.A. Punchard, J.G.L. Mullins and C. Paul

46 Computer simulations of spermine-induced A-form stabilisation in GC-rich DNA

J.S. Uretsky, A.N. Real and R.J. Greenall

47 SPD-C2-BODIPY, a fluorescent probe of spermidine transport is internalized into acidic vesicles via a two-step mechanism

D. Soulet, B. Gagnon, M. Audette, S. Rivest and R. Poulin

48 Putrescine regulates neurogenesis in the adult rodent brain

J. Malaterre, M. Cayre, R. Augier, C. Strambi, A. Strambi and G. Rougon

49 Polyamine profiles in the peripheral blood leucocytes of childhood acute lymphoblastic leukemia patients

D. Buyuktuncer, B. Cirakoglu, N. Palavan-Unsal, A. Ozalpan and K. Ozdilli

50 Polyamine homeostasis in mice with the targeted disruption of spermidine/spermine N1-acetyltransferase gene; effects of carbon tetrachloride in liver

K. Niiranen, S. Suppola, M. Pietilä, T.A. Keinänen, L. Alhonen and J. Jänne

51 Increased insulin sensitivity despite of lipoatrophy in transgenic mice overexpressing spermidine/spermine N1-acetyltransferase

E. Pirinen, S. Heikkinen, A. Virkamäki, E. Hohtola, M. Pietilä, J. Jänne and M. Laakso

Human Ageing: from the Bench to the Clinic

Poster Session Time:

52-72: Monday 16 December 14:50-16:20

52 Imaging of biological tissue using Monte Carlo simulation

A.K. Aggarwal

53 Serum bile acid profiling: an enzymatic approach

S.A. Birkensteins, S.M. Fernandez-Diaz, G.M. Murphy and R. Deane

54 DHEA regulates neutrophil function and apoptosis directly via PKC and PKB signalling pathways

D. Lascelles, K. Wang, S. Butcher and J.M. Lord

55 Neutrophil superoxide generation and phagocytosis are reduced in elderly trauma patients: relation to increased infection rates.

S.K. Butcher, H. Chahal, V. Killampali, E.K. Alpar and J.M. Lord

56 Polymorphisms of gene cluster Apo AI /CIII /AIV in patients with coronary artery disease

M. Firoozrai, A. Ehsani and B. Hesabi

57 Age effects on antibody affinity maturation

D.K. Dunn-Walters, M. Banerjee and R. Mehr

58 Dynamical systems theory and healthy ageing

M. Kyriazis

59 Cloning and characterisation of a CCR9/IL-7 fusion protein

S. Henson and R. Aspinall

60 A critique of the demographic evidence that the oldest old flies and humans age very slowly

A.D.N.J. de Grey

61 Antioxidant role of cytosolic NADP1-dependent isocitrate dehydrogenase

S.M. Lee and J.W. Park

62 Free radical production in Drosophila mitochondria

S. Miwa and M.D. Brand

63 Age-related decline in cerebrospinal fluid transthyretin (thyroid binding protein).

R.L. Chen, M.B. Segal and J.E. Preston

64 Changes in amyloid precursor protein processing in herpes simplex virus type 1 infected neuroblastoma cells.

C.B. Dobson, R.F. Itzhaki and S. Shipley

65 Skeletal muscles of aged male mice fail to adapt following contractile activity

A. Vasilaki, L. Iwanejko, F. McArdle, M.J. Jackson and A. McArdle

66 Effect of antioxidant supplementation on the ability of cells from older people to respond to oxidative stress

Y. Cheng, M.A. Gosney, F. McArdle, M.J. Jackson and A. McArdle

67 Voltammetric behaviour of salen-derived superoxide dismutase/catalase mimics

F. Fucassi, P.J. Cragg, M.H.L. Green and D. O'Hare

68 The effects of N-tert-alpha-phenyl-butylnitrone or carnosine on the age-related accumulation of oxidative DNA damage, replicative capacity and lifespan of human CD4+ T cell clones in vitro

P. Hyland, O. Duggan, K.E. Annett, C. Barnett and Y. Barnett.

69 Variance in intracellular apoptotic pathways of T cells derived from young and old donors

N.S. Della Valle, R.N. Barcia, R.W. Jones and J.D. McLeod

70 A study of mitochondrial DNA mutation in peripheral lymphocytes in an ageing cohort

B. Zhang, S. Ye, A.A. Sayer, S.R. Hammans, S. Adio, L.J. Hinks, P.J. Smythe, D. Groot, C. Cooper and I.N.M. Day

71 Changes in tropomyosin isoform expression with healthy and unhealthy ageing

S.A. Dunn, A. Advani, S.M. Marshall, A.K. Daly and T.H. Thomas

72 Overexpression of HSP70 in skeletal muscle modulates oxidative stress

C.S. Broome, M.J. Jackson and A. McArdle

 

Late breaking abstracts:

126 A novel RONS scavenging metal binding peptoid useful for assaying the mediators of oxidative stress

A.E.O. Fisher and D.P. Naughton

127 A stochastic model of somatic mutations as a cause of cellular ageing

T.B.L. Kirkwood and C.J. Proctor

Sulphotransferases in Glycobiology

Poster Session Time:

73-81: Monday 16 December 14:50-16:20

73 Chondroitin and heparan sulfotransferases in the injured CNS: their role in the formation of the glial scar and in the inhibition of axon regrowth

F. Properzi, L.M. Camargo, R.A. Asher and J.W. Fawcett

74 Glial cell line-derived neurotrophic factor (GDNF) binds to heparin with a particular dependence on 2-O-sulphates

S. Rickard, R. Mummery, B. Mulloy and C. Rider

75 Expression of heparan sulphate glucosaminyl 6-O-sulphotransferases in human kidney 293 cells

A.-T. Do, M. Kusche-Gullberg

76 2-O-sulphate groups promote the catalytic activity of heparan sulphate 6-O-sulphotransferases

P. Jemth, E. Smeds, A.-T. Do, H. Habuchi, K. Kimata, U. Lindahl, and M. Kusche-Gullberg

77 Immunohistochemical localisation of GLUT 2, sulfotransferase 1A1, and dehydroepiandrosterone sulfotransferase in human embryonic red blood cells

R. Gregoire, R. Hume, M. Coughtrie and A. Burchell

78 Variant heparan sulfates synthesised in mice lacking heparan sulfate 2-O sulfotransferase differentially regulate FGF signaling

S.E. Guimond, V. Wilson and J.E. Turnbull

79 Selection and application of phage display antibodies against the unusual GAG acharan sulphate (GlcNAc-IdoUA2S)n

G.B. ten Dam, M. Willemse, J.T. Gallagher, Y.S. Kim and T.H. van Kuppevelt

80 Structural variations of heparan sulphates in rat spleen

G.B. ten Dam, A. Oosterhof and T.H. van Kuppevelt

81 Expression levels of N-deacetylase/N-sulphotransferase isoforms in a mouse podocyte cell line cultured under high and low glucose conditions

J. van der Vlag, S.J. Rietjens and J.H. Berden

Research Colloquium - Biological Functions of Sulphated Glycoproteins

Poster Session Time:

82-95: Tuesday 17 December 14:50-16:20

82 Expression of perineuronal nets in the normal and injured rat CNS

K.E. Rhodes and J.W. Fawcett

83 Molecular recognition and modulation of hepatocyte growth factor activity by heparan and dermatan sulphates.

K.R. Catlow, J.A. Deakin, M. Delehedde, D.G. Fernig, M.S.G. Pavão, J.T. Gallagher and M. Lyon

84 Glycoprotein biosynthesis in human endometrium as revealed by microarray analysis of two stages of the menstrual cycle

J.D. Aplin, J. Borthwick, A. Sharkey, D.S. Charnock-Jones and S.K. Smith

85 Localisation of heparan sulfate 6-O-sulfotransferases 1, 2 and 3 in the developing mouse brain

K.J. Drummond, M. Ford-Perriss, H. Habuchi, K. Kimata, and J.E. Turnbull

86 Differential modulation of TSG-6 function by heparin

D.J. Mahoney, B. Mulloy, M.J. Forster, C.D. Blundell, E. Fries, C.M. Milner and A.J. Day

87 An inducible strategy to address the role of clusterin on prostate growth control

M. Scaltriti, N.J. Maitland, A. Caporali, M. Sharrard, A. Corti and S. Bettuzzi

87a Clusterin expression is down-regulated in transformed epithelial cells but up-regulated in fibroblasts from prostate cancer

M. Scaltriti, A. Caporali, S. Astancolle, D. D'Arca and S. Bettuzzi

88 Characterization of an inactive chitinase-related protein involved in mammary gland involution.

N.N. Aronson Jr and B.A. Halloran

89 Endothelial heparan sulphate proteoglycans bind IL-8 in the rheumatoid synovium

A.M. Patterson, L. Gardner, J. Shaw and J. Middleton

90 Role of heparan sulphate domain organization in endostatin action

J. Kreuger, T. Matsumoto, M. Vanwildemeersch, T. Sasaki, R. Timpl, L. Claesson-Welsh, D. Spillmann and U. Lindahl

91 Detecting changes in the sulphation pattern of heparan sulphate during development

C.L.R. Merry, V.A. Wilson, P.A. Hyde and J.T. Gallagher

92 A gravimetric analysis of protein-oligosaccharide interactions

T. Rudd, D.G. Fernig, J.T. Gallagher and R.J. Nichols

93 Interactions between vascular endothelial growth factor 165 and heparan sulphate

C.J. Robinson, J.T. Gallagher and S.E. Stringer

94 aDystroglycan abnormalities in patients with mutations in the FKRP gene

S.C. Brown, S. Torelli, C. Jiminez, C.A. Sewry, L. Feng, M. Brockington and F. Muntoni

95 Phage display-derived antibodies against human lung heparan sulfates

N.C. Smits, P.N.R. Dekhuijzen and T.H. van Kuppevelt

 

Late breaking abstract:

128 Characterizing the sulfated glycans derived from acrosome reaction-inducing substance in the starfish, Asterias amurensis

J. Gunaratne, M. Matsumoto and M. Hoshi

Drug Discovery and Design

Poster Session Time:

96-99: Tuesday 17 December 14:50-16:20

96 Inhibitory effects of somatostatin and the synthetic analogue BIM 2368 on insulin and amylin secretion: potential therapeutical approach.

M. Stojkovic, P.M. Barling, D. Naot and G.J.S. Cooper

97 Nature of O2, CO and CN binding to hemoprotein models

F. Torrens

98 Generation of QSARs, combining Physical and Field descriptors, for inhibitors of the cytochrome bc1 complex

T.E. Wiggins and M.G.B. Drew

99 Use of the simultaneous search method to develop inhibitors in silico for the Qi-site of mitochondrial cytochrome bc1 complex

T.E. Wiggins and M.G.B. Drew

 

Late breaking abstract:

129 Pharmacokinetics of OGT 918 (miglustat, Nbutyldeoxynojirimycin) in Fabry Disease

D. Karetova, E.M. Kaye, C. Clinkingbeard and J. Bultas

Proteasome Interactions with Viral and Cellular Proteins

Poster Session Time:

100-109: Tuesday 17 December 14:50-16:20

100 The S7 ATPase of the 26S proteasome interacts with the KDEL receptor

S.A. Saleh, M. Mee, S. Dawson and R.J. Mayer

101 20S proteasome biogenesis and the HIV Tat protein

G.S. Apcher, J. Maitland, S. Badaoui, H.P. Schmid, P.M. Kloetzel and R.J. Mayer

102 MEKK3 physically interacts with the PA28g regulatory subunit of the proteasome

C. Hagemann, R. Patel and J.L. Blank

103 The S8 ATPase of the 26S proteasome interacts with the Homer-3 proteins; implications for metabotropic glutamate receptor signalling

K.H. Rezvani, M.P. Mee, S.P. Dawson and R.J. Mayer

104 Inhibition of MHC class I antigen presentation by a viral ubiquitin E3-ligase

E.W. Hewitt, L. Duncan, D. Mufti, J. Baker, P.G. Stevenson and P.J. Lehner

105 The B-subunit of E. coli heat-labile enterotoxin enhances the immunogenicity of EBV latent membrane proteins

K.W. Ong, A.D. Wilson, T.R. Hirst and A.J. Morgan

106 Parkin, a-synuclein and the proteasome

S. Wray, H.C. Ardley, J.R. Cavey, P.A. Robinson and R. Layfield

107 Interaction between Epstein-Barr Virus (EBV) nuclear antigens and the proteasome

J. Heaney, R. Touitou and M.J. Allday

108 Interaction between human papillomavirus E7 protein and proteasomes.

A.J. Pemberton, D.M. Shirley, A.R. Hearn, S.R. Watters and A.J. Rivett

109 Refolding and characterization of recombinant Hepatitis B virus X antigen

Y.-Y. Cheung and K.-B. Wong

 

Late breaking abstract:

130 Confirmation of newly discovered protein interactions involving both: the splicing machinery and the ubiquitin system

M. Loescher, M. Wostry, J. Grillari and H. Katinger

Summer Vacation Studentships

Poster Session Time:

110-115: Wednesday 18 December 10:20-11:50

110 Effects of apoptotic cleavage fragments of translation initiation factor eIF4G on protein synthesis

E. Tidey, W. Wood, M.J. Coldwell, G.A. Carpenter, S.J. Morley and V.M. Pain

111 Dissecting the roles of individual collagen receptors on nitric oxide synthesis in blood platelets

V.S. Lim, W. Roberts and K.M. Naseem

112 Properties of the tungsten-substituted periplasmic nitrate reductase from Paracoccus pantotrophus

A.J. Gates, R.O. Hughes, S.R. Sharp, B. Jepson, D.J. Richardson and C.S. Butler

113 Tagging of an E. coli electron transport complex for FRAP studies of protein diffusion

P. Judge and C.W. Mullineaux

114 Molecular basis of nemaline myopathy

S.A. Johnston, C. Costa and L.M. Machesky

115 Is p53-dependent transcription required for E7-induced p53-dependent?

K. Simpson

Miscellaneous

Poster Session Time:

116-119: Wednesday 18 December 10:20-11:50

116 Targeting of protein kinase C to lipid rafts

J.D. Dukes, S.J. Slater and C.D. Stubbs

117 A model system to study polypeptide association processes in membrane proteins

A.P. Vegh and B. Robert

118 Methylation and loss of heterozygosity in cyld, a tumour suppresser in Brooke-Spiegler syndrome

C.D. Fenske, C. Pipinikas, L.A. Gunaratne, A. Chamberlain, P. Banerjee and N.D. Carter

119 Aging, amyloid b-peptide and brain mitochondria dysfunction

P.I. Moreira, M.S. Santos, A. Moreno and C. Oliveira

 

Late breaking abstracts:

131 The effect of homocysteine supplementation on s-amino acid metabolism in 3T3 cells

A.A. Drbal, V. Pavillard, R.M. Philips and A. Nicolaou

132 PTH stimulation of Saos-2 osteoblast-like cells; evidence forpotassium channel involvement.

F. Goodyear, F. McDonald and A. Warley

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