10th Nitrogen Cycle Meeting 2004

University of East Anglia, Norwich, UK

2 - 4 September 2004

The biogeochemical nitrogen cycle involves the inter-conversion of nitrogen species between a number of redox states and includes the processes denitrification, nitrification, nitrogen fixation and ammonification. Reductive and oxidative reactions involving inorganic nitrogen species provide a rich variety of systems with which to integrate studies on biochemistry, bioenergetics, molecular genetics and whole cell physiology with the environmental impacts and industrial applications of the processes. This meeting will provide an excellent opportunity for those with interests in redox reactions of the nitrogen cycle to meet and discuss recent advances in the field. The aim of the meeting will be to encourage younger scientists to present their current work in an informal setting and to stimulate the flow of current thoughts and ideas. The young scientists will all talk in sessions in which one or more contributions will also be made by invited speakers with high international profiles in the field. The proposed sessions will include; Biochemistry of NO_x reduction and oxidation; Regulation of nitrogen metabolism; Anaerobic and aerobic ammonium oxidation; Applied and environmental aspects of N-cycle redox reactions.

Proceedings will be published in Biochemical Society Transactions

POSTER ABSTRACTS

Abstracts first published on the Internet 16 August 2004

Poster Session Times:

Thursday 2 September: 18:15–19:15

Friday 3 September 12:25–14:00

Saturday 4 September 12:20–14:00

P001 Biochemical analysis of TorD: a chaperone involved in protein targeting and cofactor loading

Kostas Hatzixanthis, David J. Richardson and Frank Sargent

P002 Transient oxo-ferryl tryptophan cation radicals in cytochrome aa3 and bo3 oxidase from P.denitrificans and E.coli

F.G.M. Wiertz, O.-M. Richter , A.V. Cherepanov, B. Ludwig and S. de Vries

P003 A stable isotopic approach to elucidating organic nitrogen cycling

Hazel Mottram, Alison Thomson, Saran Sohi and Richard Evershed

P004 Reactions of Paracoccus pantotrophus N2O reductase with cytochrome c

T. Rasmussen, T. Brittain, B.C. Berks, N.J. Watmough and A.J. Thomson

P005 Discrimination between nitrate and nitrite by the model laboratory organism, Escherichia coli, and the obligate human pathogen, Neisseria gonorrhoeae

Rebekah N. Whitehead and Jeff A. Cole

P006 The FNR regulon and other anaerobically induced genes in Neisseria gonorrhoeae: from genomics and proteomics to physiological roles

Tim Overton and Jeff Cole

P007 Function of individual nap gene products in NapC-independent nitrate respiration of Wolinella succinogenes

Roland Gross, Melanie Kern, Monica Sänger and Jörg Simon

P008 Alkaline cyanide biodegradation by Pseudomonas pseudoalcaligenes CECT5344

V.M. Luque-Almagro, R. Blasco, M.J. Huertas, M. Martínez-Luque, C. Moreno-Vivián, F. Castillo and M.D. Roldán

P009 N-oxides sensing in pseudomonas aeruginosa: expression and characterization of dnr, a fnr-crp type transcriptional regulator

Serena Rinaldo, Giorgio Giardina, Maurizio Brunori and Francesca Cutruzzolà

P010 Reactivity of pseudomonas aeruginosa nitrite reductase

Centola Fabio, Rinaldo Serena, Maurizio Brunori and Francesca Cutruzzolà

P011 Multiple Forms of Oxidised Nitric Oxide Reductase

Jessica H. van Wonderen, Sarah J. Field, David J. Richardson, Vasily S. Oganesyan, Myles R. Cheesman and Andrew J. Thomson

P012 The role of NapD in the maturation of NapA

Emily R. Leach, Heather Sears, Stuart Ferguson, Jeffrey A. Cole and David J. Richardson

P013 Poster withdrawn

P014 A novel method of variant cytohrome cd1 expression

Richard Zajicek

P015 Cytochrome c Nitrite Reductase – An Enzyme Chameleon

James D. Gwyer, David J. Richardson and Julea N. Butt

P016 Characterisation of Escherichia coli NrfB: a deca-heme homo-dimer that transfers electrons to a deca-heme nitrite reductase

Tom Clarke, Vikki Dennison, Harriet Seward, Benedicte Burlat, Jeffery Cole, Andrew Hemmings and David Richardson

P017 Roles of nitrate and nitrite reductases in Campylobacter jejuni

Marc S. Pittman and David J. Kelly

P018 Proteome analysis of the anammox bacterium Kuenenia stuttgartiensis

Irina Cirpus, Huub Op den Camp, Marc Strous and Mike Jetten

P019 Biogeochemistry and microbial ecology of metal-sulfur-nitrogen interactions in wetlands

S.C.M. Haaijer, M.E.W. van der Welle, L.P.M. Lamers, M.S.M. Jetten, J.G.M. Roelofs and H.J.M. Op den Camp

P020 DNA binding properties of the Escherichia coli nitric oxide sensor NorR: towards an understanding of the regulation of flavorubredoxin

Nicholas Tucker, Benoit D’Autréaux, Stephen Spiro and Ray Dixon

P021 Distribution and diversity of the nrfA gene encoding nitrite reduction to ammonia in estuarine sediments

J. Takeuchi, D.B. Nedwell, S.B. Mohan, J.A. Cole and A.M. Osborn

P022 Nitrate and nitrite transport in Escherichia coli

Wenjing Jia and Jeff Cole

P023 Biogenesis of c-type cytochromes: the harder you look the more complex it becomes

James W.A. Allen and Stuart J. Ferguson

P024 Current thoughts on bacterial nitrate reductases: The voltammetric characterisation of NapAB from Paracoccus pantotrophus.

A.J. Gates, D.J. Richardson and J.N. Butt

P025 Nitrate Reduction in Streptomyces coelicolor

J. White, J. Alderson and R.G. Sawers

P026 Using Artemis to root around in the genome sequence of Rhizobium leguminosarum

Lisa C. Crossman, Julian Parkhill and the Pathogen Sequencing Unit

P027 Application of anammox bacteria

Boran Kartal, Laura van Niftrik, Wouter van der Star, Mark van Loosdrecht, Marc Strous and Mike Jetten

P028 Phylogenetic markers for the assessment of ammonium oxidizing bacteria: hao/hzo versus amoA and 16S rRNA

M. Schmid, I. Cirpus, K. van der Pas-Schoonen, H. op den Camp, M. Strous and M. Jetten

P029 Physico-chemical Studies of Cytochrome c Nitrite Reductase (NrfA) from Escherichia coli

Bénédicte Burlat, James Gwyer, Suzanna Poock, Tom Clarke, Myles Cheesman, Julea Butt, Andrew Hemmings and David Richardson

P030 The Role of Denitrification in Nitrite and Nitric Oxide Detoxification in Soybean Nodules

Georgina E. Meakin, María J. Delgado, Brian Jepson, Eulogio J. Bedmar and David Richardson

P031 Anammox in the past oceanic nitrogen cycle

Jack van de Vossenberg, Markus Schmid, Mike Jetten, Jaap Sinninghe Damsté and Marc Strous

P032 Characterization of the nirK gene of Rhizobium etli CFN42

Emilio Bueno, Nicolás Gómez, Lourdes Girard, Eulogio J. Bedmar and María J. Delgado

P033 Molybdate transport in Bradyrhizobium japonicum

Chouhra Talbi, Alvaro Tresierra-Ayala, Nuria Molinero, Eulogio J. Bedmar and María J. Delgado

P034 Nitrate reduction in the periplasm of Escherichia coli

Brian Jepson, Sudesh Mohan, Andrew Hemmings, Jeff Cole and David Richardson

P035 Nitric Oxide Detoxification in Salmonella enterica serovar Typhimurium

Paul Mills, David Richardson, Steve Spiro and Jay Hinton

P036 Nitrate reductases from Paracoccus denitrificans and Enterobacter cloacae; different activities towards nitrate and selenate

Carys A. Watts , Helen Ridley , Ann J. Reilly, David J. Richardson and Clive S. Butler

P037 Selenate respiration in the hyperthermophilic Archaea

Elizabeth J. Dridge and Clive S. Butler

P038 Nitrate and selenate respiration in Aeromonas hydrophila

James T. Leaver and Clive S. Butler

P039 Transcriptional Regulation and Physiological Studies of a Hybrid Cluster Protein

N.A. Filenko and J.A. Cole

P040 Electron Input Pathways in a Bacterial Nitric Oxide Reductase

Faye Thorndycroft and Nicholas J. Watmough

P041 From Nitrite Binding to Nitric Oxide release; the first turnover of cytochrome cd1

Andrey D. Matorin and Nicholas J. Watmough

P042 Reduced and Oxidised Structures of ResA reveal a Redox-Driven Protein Substrate Selection Mechanism in Cytochrome c Biosynthesis

Allister Crow, Richard Acheson, Nick Le Brun and Arthur Oubrie

P043 Micro-aerobic denitrification in Neisseria meningitidis.

Jonathan D. Rock and James W.B. Moir

P044 Investigating NarR, the third FNR-type Regulator of Paracoccus

Matthew F. Ewles and James W.B. Moir

P045 Purification and characterisation of cytochrome c’ from Neisseria meningitidis

Wilhelmina M. Huston, Elisabeth C. Lowe, Clive S. Butler and James W.B. Moir

P046 Role of lipid-modified azurin (Laz) in electron transport of Neisseria meningitidis

M. Deeudom, W. Huston and James W.B. Moir

P047 Ammonium sensing in E. coli

Arnaud Javelle and Mike Merrick

P048 Transcriptional regulation of nitric oxide reduction in Ralstonia eutropha

Andrea Büsch, Katja Strube, Bärbel Friedrich and Rainer Cramm

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