Molecular Mechanisms of Neurodegeneration: a Joint Biochemical Society/Neuroscience Ireland Focused Meeting (and satellite symposium)

O’Reilly Hall, University College Dublin, Republic of Ireland

14 - 16 March 2005

POSTER ABSTRACTS

Abstracts first published on the Internet 4 March 2005

Poster Session Times:

P001-P045

Tuesday 15 March 13:05-15:00, 17:30-18:30

P046-P091

Wednesday 16 March 13:05-15:00, 17:30-18:30

P001 BACE1 and BACE2 isoform expression and b-secretase enzyme kinetics are altered in the Alzheimer’s disease temporal cortex

J. Stockley, R. Ravid and C. O’Neill

P002 b-Secretase activity in human platelets

W.W. Liu, S.A. Todd, D.T.R. Coulson, S. Murphy, G.B. Irvine, A.P. Passmore and J.A. Johnston

P003 b-Secretase activity is not required for generation of the intracellular C-terminal domain of the APP family of proteins

J.V. Fadeeva, D.M. Walsh, J. Tang, Y. Luo, P. Paganetti, M. Citron, W. Wasco and D.J. Selkoe

P004 Hypoxic modulation of amyloid production in primary cultures of rat cortical astrocytes: a role for b-secretase (BACE1)?

J.P. Boyle, C. Stanton and C. Peers

P005 b-Secretase (BACE1) expression in primary cultures of rat cortical astrocytes

J.P. Boyle, C. Stanton and C. Peers

P006 Association of BACE with late onset Alzheimer’s disease

A. Keown, S. Todd, S. McIlroy and P. Passmore

P007 Inhibition of aspartyl proteases

S. Cosgrove, E. Spink, L. Rogers and J.P.G. Malthouse

P008 Uncovering the mechanistic basis of insulin-degrading enzyme’s sensitivity to thiol-alkylating agents

M.A. Leissring, T.E. Logan, X. Wu, W. Farris and D.J. Selkoe

P009 Ab peptides bearing the Flemish mutation are more slowly degraded by neprilysin than wild type Ab

V. Betts, J.V. Fadeeva, M.A. Leissring, F. Grueninger, C. Adessi, D.J. Selkoe and D.M. Walsh

P010 Expression of the APP intracellular domain (AICD) potentiates ER stress mediated apoptosis

C.G. Concannon, T.H. Mueller, D. Kögel, R. Egensperger and J.H.M. Prehn

P011 Using yeast as a model system for studying synthesis and degradation of APP fragments

L.J. Sparvero, S. Patz and C.M. Coughlan

P012 Molecular interactors influencing APP metabolism

E. Marcello, F. Gardoni, D. Mauceri, M. Zimmermann, F. Cattabeni and M. Di Luca

P013 Dysregulation of caveolin processing in Alzheimer disease mice

M.A. Chishti, M. Ahmed, A. Laualich, S. Nakeeb, J. Yang, R. Strome and D. Westaway

P014 Partitioning of the serotonin transporter into cholesterol-enriched lipid microdomains modulates transport of serotonin

F. Magnani, C. Williams and J. Haase

P015 Generation and characterization of mutant cell lines defective in g-secretase processing of Notch and APP

A. Olry, P. Chastagner, A. Israël and C. Brou

P016 A putative new role for presenilin-1 in IL1R-signalling: linking IL1R signalling to Alzheimer’s disease

B.M. Elzinga, J.C. Powell, F. Harte, C. Twomey and J.V. McCarthy

P017 Phosphorylation of presenilin 1 regulates binding and turnover of beta-catenin

K. Prager, R. Fluhrer, L. Wang-Eckhardt and J. Walter

P018 A novel role for presenilins in NGF-signalling: relevance to the pathogenesis of Alzheimer’s disease

J.C. Powell, B.M. Elzinga, C. Twomey and J.V. McCarthy

P019 The initial substrate binding site of g-secretase is located on presenilin near the active site

A. Kornilova, F. Bihel, C. Das and M. Wolfe

P020 Calmodulin binding to APP and the APLPs

M.A. Myre, R.E. Tanzi, G. Tesco and W. Wasco

P021 Degradation of APP during the early phase of long-term memory consolidation

L. Conboy, N.C. O’Sullivan, K.J. Murphy and C.M. Regan

P022 Systemic passive immunization prevents the inhibitory effect of amyloid beta oligomers on hippocampal LTP in vivo

I. Klyubin, D.M. Walsh, R. Anwyl, G.M. Shankar, D.J. Selkoe, T. Wisniewski and M.J. Rowan

P023 Working memory deficit and increased theta oscillations in metabotropic glutamate receptor 7 KO mice

C. Holscher, S. Schmid, P. Pilz, G. Sansig, H. Van Der Putten and C. Plappert

P024 Memory impairment in a novel mouse model of human tauopathy

M. Ramsden, L. Kotilinek, J. Paulson, A. Guimaraes, C. Forster, K. SantaCruz and K.H. Ashe

P025 Specific amyloid-b assemblies disrupt memory without neurodegeneration in a mouse model of Alzheimer’s disease

S. Lesné, L. Kotilinek, R. Kayed, C.C. Glabe and K.H. Ashe

P026 Analysis of amyloid-b peptide composition in amyloid deposits in human Alzheimer’s disease brains and in transgenic mice

A. Guentert, C. Czech and B. Bohrmann

P027 The effects of soluble and aggregated Ab(1-40) on LTP in the rat CA1 in vivo

A.W. Schmid, M.A. Lynch and C.E. Herron

P028 Structural studies on cystatin amyloid fibrils

G.J. Morgan, E. Zerovnik, J.P. Waltho and R.A. Staniforth

P029 Harnessing chaperones to generate potent small molecule inhibitors of amyloid b aggregation

J. Gestwicki, M. Bose, V. Devasthali, G. Crabtree and I. Graef

P030 A strategy for designing inhibitors of a-synuclein aggregation and toxicity as a novel treatment for Parkinson’s disease

O.M.A. El-Agnaf, K.E. Paleologou, D. Allsop and F.L. Martin

P031 Retro-inverso peptides are highly potent neuroprotective agents against the toxicity of soluble b-amyloid oligomers

B.M. Austen, B. Maltharu, A. Siddiqui, G. Gibson and O.M. El-Agnaf

P032 Prefibrillar oligomers of human stefin B (cystatin B) interact with membranes

G. Anderluh, I. Gutirrez-Aguirre, S. Rabzelj and E. Zerovnik

P033 Regulation of CREB-dependent gene expression in hypoxia

K. Comerford, E. Cummins, M. Leonard and C. Taylor

P034 Toxic effects of expanded polyalanine repeats in Drosophila

Z. Berger, J.E. Davies, C.J. O’Kane and D.C. Rubinsztein

P035 A mutation in dynein rescues axonal transport defects and extends the lifespan of ALS mice

D. Kieran, S. Bohnert, M. Hafezparast, J. Martin, E. Fisher, G. Schiavo and L. Greensmith

P036 Angiogenin gene mutations in amyotrophic lateral sclerosis

M. Greenway, S. Ennis, M. Alexander, B. Traynor, R. Swingler, A. Green and O. Hardiman

P037 Exogenous hsp27 rescues motoneurons from cell death induced by apoptotic stimuli

B. Kalmar and L. Greensmith

P038 Microarray analysis of multiple sclerosis brain tissue

M. Padden, S. McQuaid and B. Brankin

P039 A combined gene therapy approach to axon regeneration using lentiviral vector delivery of siRNA and neurotrophin

N. O’Callaghan, N. Madigan, E. Donnelly, T. O’Brien and P. Strappe

P040 Axonal transport and behavioural defects in Drosophila expressing wild type and mutant tau

A. Mudher, F. Chee, D. Mackay, S. Lovestone, T. Newman and D. Shepherd

P041 Poster withdrawn

P042 JNK regulates dendritic architecture: role of MAP2 as an effector

B. Björkblom, N. Östman, V. Hongisto, V. Komarovski, T. Kallunki, M.J. Courtney and E.T. Coffey

P043 Epigenetic regulator proteins for phenotype alterations and proteinaceous transmission of conformational/prion diseases (CD) and delayed-type hypersensitivity (DTH) are structurally related by domains for binding endogenous redox- and metalloregulated RNA bioaptamers

J.H. Wissler

P044 Neurochemical changes associated with prion using the murine ME7 scrapie model

V. O’Connor, B.C. Gray, C. Cunningham and V.H. Perry

P045 Alpha-synuclein is a high affinity copper binding protein

J. King, F. Benson, M. Cundell, A. Thompsett, D. Brown and D. Allsop

P046 Activation of p75NTR is linked to functional impairment in the rat hippocampus

A. Hennigan and A. Kelly

P047 Peroxisome proliferator activated receptor agonists modulate synaptic transmission and beta amyloid (Ab 1-40) mediated impairment of long-term potentiation in the rat CA1 in vitro

D.M. O’Leary, D.A. Costello and C.E. Herron

P048 Dexamethasone and vitamin D3 attenuate the LPS-induced microglial activation in an IL-10-dependent manner in vitro

A. Piazza, M. Moore and M.A. Lynch

P049 Enhanced susceptibility of rat hippocampus to Ab with age

A.M. Minogue, C.E. Herron and M.A. Lynch

P050 The effects of IL-1ra on beta amyloid mediated depression of LTP in the rat CA1 in vivo

A.W. Schmid, M.A. Lynch and C.E. Herron

P051 Studies on the transcriptional regulation of cholesterol 24-hydroxylase: a key regulator of brain cholesterol homeostasis

Y. Ohyama, S. Meaney, M. Heverin, A. Brafman, M. Shafir, U. Andersson, M. Olin, G. Eggertsen, U. Diczfalusy, E. Feinstein and I. Björkhem

P052 Statins act to suppress microglial activation in lipopolysaccharide-treated rat hippocampus

R. Clarke and M. Lynch

P053 The anti-inflammatory cytokine IL-4 abrogates b-amyloid induced changes in the rat brain

A. Lyons, R. Clarke and M. Lynch

P054 Effects of TNF-a on NMDA inhibition of evoked dopamine release in rat striatum in vitro: a study using FCV

C. O’Neill and J.J. O’Connor

P055 Eicosapentaenoic acid confers neuroprotection in the rat hippocampus

A.M. Lynch, A.M. Minogue, Ó. Roche and M.A. Lynch

P056 mGluR5 inhibition reverses the inhibitory effect of tumour necrosis factor-alpha on long-term potentiation

D. Cumiskey, M.P. Butler, P.N. Moynagh and J.J. O’Connor

P057 Cell cycle-related signalling in Alzheimer’s disease type neurodegeneration

K.F. Gallagher and H.C. Gallagher

P058 The age-related changes in the hippocampal concentration of interleukin-1b and interleukin-1b-induced signalling are attenuated by the synthetic corticoid, dexamethasone

M. Moore and M. Lynch

P059 Crossing the barrier: net flux of 27-hydroxycholesterol into the human brain

M. Heverin, S. Meaney, D. Lütjohann, U. Diczfalusy, J. Wahren and I. Björkhem

P060 Role of microglial activation in the inhibition of LTP induced by amyloid beta (Ab)

C.A. Callaghan, C.E. Costelloe, A.L. Lyons, A.M. Minogue and M.A. Lynch

P061 Potentiation of microglial inflammatory response by protein acetylation

J. Huuskonen, T. Suuronen and A. Salminen

P062 Regulation of clusterin expression by protein acetylation in neural cells

T. Nuutinen, T. Suuronen, J. Huuskonen, S. Kyrylenko and A. Salminen

P063 Primed microglia in the brain: an accident waiting to happen

C. Cunningham and V.H. Perry

P064 Ab regulates the lysosomal system via p53 in cultured cortical neurons

R.M. McCormack, M.P. Fogarty and V.A. Campbell

P065 Are shortfalls in the cell cycle and tau-regulating protein Pin1 a susceptibility factor for neurodegeneration and ageing?

L. Hashemzadeh-Bonehi, S. Mosaheb, N.J. Cairns, S.L. Rulten, J.E. Kay, R.G. Phillips and J.R. Thorpe

P066 Dying to survive: increased Akt activation with loss of PTEN and Akt are features of Alzheimer’s disease pathogenesis

A. Moloney, R. Griffin, M. Kelliher, J.A. Johnston, R. Ravid, R. O’Connor and C. O’Neill

P067 Increased IGF-1 receptor levels, decreased IGFBP-2 levels, and defects in normal IGF-1 and insulin receptor signalling are associated with the pathogenesis of Alzheimer’s disease

R. Griffin, A. Moloney, M. Kelliher, J.A. Johnston, R. Ravid, R. O’Connor and C. O’Neill

P068 Bim regulation in seizure-induced neuronal death and epilepsy

S. Shinoda, C.K. Schindler, R. Meller, N.K. So, T. Araki, A. Yamamoto, J.-Q. Lan, W. Taki, R.P. Simonand D.C. Henshall

P069 Induction of intrinsic mitochondrial cell death pathway following experimentally evoked seizures and in human temporal lobe epilepsy

B. Murphy, A. Yamamoto, C.K. Schindler, R.P. Simon and D.C. Henshall

P070 Real-time single cell analysis of Bid cleavage and translocation to the mitochondria in neurons during excitotoxic neurodegeneration

M.W. Ward and J.H. Prehn

P071 Cellular and sub-cellular calcium accumulation during glutamate induced injury in cerebellar granule neurons

M.W. Ward, S. Greenwood and C.N. Connolly

P072 Dietary enrichment with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) reverses age-related decreases in the GluR2 and NR2B glutamate receptor subunits

S. Dyall, G. Michael and A. Michael-Titus

P073 Traumatic injury to the medial prefrontal cortex results in immediate and prolonged increases in glutamate, aspartate and GABA release remote from the site of injury

A. Smyth, M. Gilchrist and W.T. O’Connor

P074 Effects of full and partial dopamine D2 receptor agonists on striopallidal GABA transmission in the awake rat

A.M. Duffy, J.C. Glennon, G. van Scharrenburg and W.T. O’Connor

P075 The neuroprotective effect of nerve growth factor (NGF) on 6-hydroxydopamine (6-OHDA)-treated PC12 cells

J.P. Loughlin, K.M. Doyle, E. Kavanagh and A.M. Gorman

P076 Adrenomedullin and endothelin-1 mRNA in the medulla-pons during acute EAE

R. Mongru, C. Paul, C. Bolton and S. Barker

P077 Metabolic disruptions caused by gliotoxic amino acids

L. Brennan, C. Hewage, J.P.G. Malthouse and G.J. McBean

P078 Potential role of glial specific lectin in neuronal survival

T. Lekishvili and D.R. Brown

P079 Medullary pathology in carotid sinus syndrome and orthostatic hypotension

V. Miller, R.A. Kenny and R.N. Kalaria

P080 Poster withdrawn

P081 Behavioural evaluation of TRH-degrading ectoenzyme inhibitors

N. Hogan, J.A. Kelly and K.M. O’Boyle

P082 Amnesia induced by antibodies to the neural cell adhesion molecule (NCAM) is only effective for non-spatial forms of learning in the rat

C.M. Seymour, K.J. Murphy and C.M. Regan

P083 Minimal change to rodent housing affects behaviour and brain structure

C.W. Thomas, J.S. Loscher, K.J. Murphy and C.M. Regan

P084 Prevalence of cognitive impairment in haemodialysis patients

A.M. Murray, D. Knopman, D. Tupper, G. Smith, S. Pederson and D. Gilbertson

P085 Age bias delays early recognition of AD in older adults

J.R. McCarten, L.S. Hemmy, S.J. Rottunda and M.A. Kuskowski

P086 Ab oligomers and their cognitive effects

J.P. Cleary, S. Lesne, M.B. Podlisny, G.M. Shankar, D.M. Walsh, D. Selkoe and K.H. Ashe

P087 Familial Alzheimer’s disease with spastic paraparesis: two kindreds with PS-1 exon 9 deletion due to splice acceptor mutations

G.A. Broe, W.S. Brooks, J.B. Kwok, J.J. Kril and P.R. Schofield

P088 A novel panel of protein biomarkers for the diagnosis of Alzheimer’s disease

J. McGuire

P089 ADDL-directed therapeutics for treatment of Alzheimer’s disease and related memory disorders

G.A. Krafft

P090 Cyclic nucleotide gene expression: a novel approach to Alzheimer’s disease drug discovery

I. McPhee, L. Gibson, J. Kewney, P. Stevens, C. Breslin, M. McFarlane and S. MacKenzie

P091 Meptides: N-methylated peptides - comparison with other peptides and small molecule Ab aggregation inhibitors

H. Amijee, N. Kokkoni, A.J. Doig, K. Stott, J.M. Treherne and D.I.C. Scopes

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