Biometals 2002 3rd International Biometals Symposium

King’s College London, Franklin-Wilkins Building, Waterloo, London

11 - 13 April 2002

POSTER ABSTRACTS

Poster sessions

Friday 12 April 15.45–17.45

Session A: Bioremediation

A1 The arsenate reduction catalysed by S. aureus pI258 ArsC

J. Messens, J.C. Martins, K. Van Belle, E. Brosens, A. Desmyter, I. Zegers and L. Wyns

A2 Identifying the genes involved in cadmium hyperaccumulation in Thlaspi caerulescens

K. Tearall, J.R. Howarth, F.J. Zhao, S.P. McGrath and M.J. Hawkesford

A3 The single superoxide dismutase of Ralstonia metallidurans CH34

J. Coves and M. Roux

A4 Kinetics of uranyl reduction by hydrogenase systems

L.L. Barton, R.M. Plunkett and R. Narang

A5 Oxygen reactions of cellobiose oxidoreductase from Phanerochaete chrysosporium

M.G. Mason, M.T. Wilson, C.E. Cooper, A.S. Ball and P. Nicholls

A6 Oxalate oxidase from Ceriporiopsis subvermispora: a new bicupin with oxidase activity

M.R. Escutia and S. Bornemann

A7 P450 System from Acinetobacter sp. EB104: preparation, characterization and interaction of components

N. Gupta and O. Asperger

Session B: Metal Insertion into Proteins

B1 Electron transfer in mitochondrial complex I: characterization of the ÔTYKYÕ Subunit

E.J. Leggate and J. Hirst

B2 Hexa-ammine-cobalt chloride influenced the activity of Serratia marcescens nuclease

M.N. Filimonova, V.P. Gubskaya, I.A. Nuretdinov and I.B. Leshchinskaya

B3 Investigation of the succinate-quinone-reductase of the thermoacidophilic archaeon Thermoplasma acidophilum

R. Falke and S. Anemueller

B4 Characterization of the hydrogenase maturation proteins HypE and HypF

A. Paschos, A. Bauer and A. Böck

B5 Copper binding affinities of copper homeostasis proteins

P.A. Cobine, C.E. Jones and C.T. Dameron

B6 Complex formation of two components of the [NiFe]-hydrogenase maturation machinery in Escherichia coli: the HypC-HypD complex

M. Blokesch and A. Böck

B7 Analysis of the endoproteolytic cleavage of the large subunit of NiFe-hydrogenases

E. Theodoratou and A. Böck

B8 Chemical recognition of l and d octahedral metal complexes by glucose oxidase

E.V. Ivanova and A.D. Ryabov

B9 NMR provides factors responsible for the high redox potential of copper(II) rusticyanin

A. Donaire, B. Jiménez, J.M. Moratal, J.F. Hall and S.S. Hasnain

B10 Metallo-b-lactamase from Bacillus cereus: crystal structure of an active site mutant at 2.2Å resolution

A.M. Davies, A.J. Vila, B.J. Sutton and S.M. Fabiane

Session C: Metal Transport

C1 Ferromagnetic resonance signal diversity in Brazilian ants

E. Wajnberg, O.C. Alves, A.Y. Arada and D.M.S. Esquivel

C2 Migratory ant and its termite prey: ZFC and FC measurements

D.M.S. Esquivel, E. Wajnberg, G. Cernicchiaro and O.C. Alves

C3 Complete structural characterization of pyoverdins using triple resonance experiments and orientational constraints

E. Wasielewski, M.A. Abdallah and B. Kieffer

C4 Bacterial siderophores: synthesis and biological activities of novel pyochelin analogues

A. Zamri, I.J. Schalk, F. Pattus and M.A. Abdallah

C5 The relationship between iron metabolism and the growth, survival and pathogenicity of Salmonella

A.K. Robinson, P. Golby, P.A. Barrow, A. Jones, A. Pudney and S.C. Andrews

C6 Role of mucin in intestinal iron absorption

S.E. Jafri, K.B. Raja, T.J. Peters and R.J. Simpson

C7 Mucosal reductase in HFE KO and iron deficient mice

R.J. Simpson, K. Schumann, E. Debnam and S.K. Srai

C8 The role of ferric-chelate reduction in pathogenicity

C.M. Procter, J.S. Cavet, S.J. Yeaman, A.K. Robinson, S.C. Andrews and N.J. Robinson

C9 Nramp transporter of Chlamydomonas reinhardtii

A. Rosakis and W. Köster

C10 Iron-induced intestinal toxicity in human Caco-2 cells

S. Ferruzza, L. Gambling, F. Natella, Y. Sambuy, M. Scacchi and M.L. Scarino

C11 Siderophore production by the yeast Rhodotorula mucilaginosa

D. Andersen and M.G. Wiebe

C12 Site-directed mutagenesis of the iron-binding residues of ferric binding protein from Neisseria gonorrhoeae

D.J.B. Hunter, P.J. Sadler and D.J. Campopiano

C13 Zinc in trypanosomes

D.A. Scott

C14 Impaired intestinal iron transport during the acute phase reaction

G.J. Anderson, D.M. Frazer, S.J. Wilkins, A.T. McKie, E.M. Becker and T.L. Murphy

C15 Where does metal specificity reside? Metal-transport and -trafficking

G.P.M. Borrelly, S.A.M. Rondet and N.J. Robinson

C16 Metal-dependent regulation of DMT1 and IREG1 expression in Caco-2 cells

P.A. Sharp, S.Yamaji, J. Tennant and S.K.S. Srai

C17 New analogues of triscatecholate siderophores based on triamines or monosaccharides

P. Gebhardt, S. Heggemann, L. Heinisch and U. Moellmann

C18 Characterization of Mrs2p as the major Mg2+ transporter in mitochondria

M. Kolisek, D. Steffel, G. Szurka, M. Schweigel and R.J. Schweyen

C19 Bacterial Nramp transporters and virulence

M.F.M. Cellier

C20 Expression, activity and localization of recombinant hephaestin

E. Aslan, B.A. Syed, S.K.S. Srai, R.W. Evans and K.N. White

C21 Study of E. coli MntH transmembrane topology

P. Courville and M.F.M. Cellier

C22 Characterization of the cation diffusion facilitator (CDF) proteins of Arabidopsis thaliana - a role in metal homeostasis?

A.-G. Desbrosses-Fonrouge and U. Kraemer

C23 Metal metabolism in hereditary haemochromatosis

K.B. Raja, K. Srai, K. Schumann, A. Bomford, T.J. Peters and R.J. Simpson

C24 A novel family of signal transducers with a role in siderophore utilization

P.O. Cuiv, P. Clarke, C. Viguier and M. OÕConnell

C25 The effect of modulators of haem biosynthesis on intestinal iron absorption

A.H. Laftah, K. Raja, R.J. Simpson and T.J. Peters

C26 Effect of tin-metalloporphyrins on haem biosynthesis and intestinal iron absorption

A.H. Laftah, K. Raja, R.J. Simpson and T.J. Peters

C27 Analysis of the components in Fet3 protein, a multinuclear copper oxidase, required for high-affinity iron uptake in yeast

T.-P. Wang, S. Severance, L. Pajak and D. Kosman

C28 Role of copper-zinc superoxide dismutase (CuZnSOD) in zinc and other transition metal homeostasis

J.J. Wei, C. Srinivasan, E.B. Gralla and J.S. Valentine

C29 Thermodynamics of haem binding to HasAsm hemophore

N. Izadi-Pruneyre, N. Wolff, C. Deniau, R. Gilli, S. Letoffe, C. Wandersman, M. Delepierre and A. Lecroisey

C30 The homologue of Escherichia coli ferric enterobactin esterase is involved in chrysobactin-dependent iron acquisition in Erwinia chrysanthemi

L. Rauscher, B. Matzanke, A. Trautwein and D. Expert

C31 Is duodenal cytochrome B localized in the recycling endosome ?

G.O. Latunde-Dada and A.T. McKie

C32 Cu regulates Fe-dependent protein expression in a human hepatoma cell line (hepG2)

C. Fosset, B.A. McGaw and H.J. McArdle

C33 Effect of transition metal ions on intestinal iron absorption

G.O. Latunde-Dada, S. Shirali, A.T. McKie, R.J. Simpson and T.J. Peters

C34 Structural studies of meningococcal transferrin-binding protein A

J.S. Oakhill, A.R. Gorringe, C.L. Joannou and R.W. Evans

C35 Gene structure and comparative modelling of human hephaestin

B.A. Syed, N.J. Beaumont, A. Patel, C.E. Naylor, K.S. Srai and R.W. Evans

C36 The chemical nature of non-transferrin bound iron

A. Zarea, R. Evans and R.C. Hider

C37 Cu interaction and complexation with homocysteine and its physiological action

M. Apostolova, B. Ivanova, W. Russell, M. Arnaudov, J. Beattie, P. R. Bontchev and C. Nachev

C38 Characterization of the zinc-binding, yeast mitochondrial TIM10 translocase complex by biophysical techniques

H. Lu, S. Vial, P. Savory and K. Tokatlidis

C39 Design of iron-specific fluorescent probes for monitoring intracellular labile iron pools

Z.D. Liu and R.C. Hider

Session E: Metal Toxicity and Therapeutic Intervention

E1 Induction of apoptosis by iron chelators

J.L. Buss, J. Neuzil and P. Ponka

E2 The role of the prion protein in cellular copper and zinc homeostasis

N.T. Watt, W.S.S. Perera and N.M. Hooper

E3 Lead and cadmium levels in whole blood of patients with chronic tubulointerstitial nephritis

A.A. Rapondjieva, B.D. Atanasova, I.K. Kaludina, V.D. Vasilev and K.N. Tzatchev

E4 Structure, reactivity and cytotoxicity of tripodal chelators and complexes: the effects of heterocyclic donor groups and enforced coordination geometry on Fe(II) complexation by potential antitumour agents

R.P. Planalp, S. Lai, G. Lu, A.M. Przyborowska, G. Park, R.D. Rogers, G.A. Broker, M.W. Brechbiel, S.V. Torti and R. Ma

E5 Metal-promoted phosphate diester hydrolysis by novel Cu(II) complexes of tris(N-alkylated) cis,cis-1,3,5-triaminocyclohexane

R.P. Planalp, K.A. Deal, G. Park, J. Shao, F.H. Lu, R.D. Rogers and M.W. Brechbiel

E6 Non-transferrin-bound iron, transferrin saturation and oxidative stress in ESRD-patients receiving intravenous iron therapy

B. Scheiber-Mojdehkar, B. Lutzky, R. Schaufler, F.T. Meisl and H. Goldenberg

E7 Synergism of iron chelators

L.D. Devanur and R.C. Hider

E8 No oversaturation of transferrin after i.v.-iron-administration in ESRD-patients

B. Lutzky, R. Schaufler, F.T. Meisl, H. Goldenberg and B. Scheiber-Mojdehkar

E9 Differences in the iron chelation efficacy between deferoxamine and deferiprone

R. Fischer, F. Longo, R. Engelhardt, A. Piga and P. Nielsen

E10 Generation of oxygen radicals stimulates the uptake of non-transferrin bound iron by increasing labile intracellular iron

S. Czingraber, B. Sturm, H. Goldenberg and B. Scheiber-Mojdehkar

Session F: Functional Genomics and Gene Regulation in Biometals Research

F1 The effect of cobalt on cytochromes biosynthesis in Paracoccus denitrificans

Sh. Golkhoo and E. Keyhani

F2 Characterization of Staphylococcus aureus iron uptake mutants

J. Hammacott, A. Cockayne, J. Morrissey and P. Williams

F3 Modulation of oxidative stress responses by cadmium ions in Xanthomonas

P. Banjerdkij, T. Chuchue, P. Vattanaviboon, P. Parkpian, S. Loprasert and S. Mongkolsuk

F4 Heavy metal hypersensitive mutants of the unicellular green alga Chlamydomonas reinhardtii

M. Hanikenne, C. Nieberding, R.F. Matagne, M.C.S. Wu and R. Loppes

F5 Global iron dependent gene regulation in Escherichia coli

J.P. McHugh, F. Rodriguez-Qui-ones and S.C. Andrews

F6 Control of ferritin expression via nitric oxide modulation of iron regulatory protein 2

S. Kim and P. Ponka

F7 A lead specific regulator for Ralstonia metallidurans CH34

J.L. Hobman, D.J. Julian and N.L. Brown

F8 Transferrin receptor 1 and 2 expression in liver of mice with stimulated or suppressed erythropoiesis

M. Vokurka, K.-T. Chang and E. Necas

F9 A new transcriptional response to nitrosative stress: direct Fur inhibition by nitric oxide via nitrosylation of the iron site

B. DÕAutreaux, D. Touati, B. Bersch, J.M. Latour and I. Michaud-Soret

F10 Expression of proteins involved in iron metabolism in scrapie-infected neuroblastoma cells

S. Fernaeus, C. Pettersson, K. Bedecs and T. Land

F11 Expression and regulation of human cysteine desulphurase, IscS

J. Hälldin, S. Fernaeus and T. Land

F12 Cross-species comparison of copper metabolism genes by whole-genome sequence analysis

H.H.M.J vanBakel, C. Wijmenga and M. Huynen

F13 Hepcidin expression in the liver during hypoxia

S. Miret, R. Simpson and A. McKie

F14 Host iron-signaling altered by pathogenic Neisseria infection

R.A. Bonnah, C.A. Enns and M. So

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