Signalling the Future

University of Liverpool, UK

3 - 6 September 2002

For full programme information visit www.signal2002.com

Speaker Abstracts

Poster Abstracts

Speaker Abstracts

P2 Phosphatidylinositol 3,5-bisphosphate, a 'novel' phospholinositide involved in intracellular trafficking

R.H. Michell and S.K.Dove

P3 Structural Biology of Multiprotein Complexes involved in Extracellular, Cytoplasmic, and Nuclear Signals.

Tom L. Blundell

P4 Controlling the Cell Cycle

Paul M Nurse

P5 LIVERPOOL; THE EARLY YEARS OF BIOCHEMISTRY

G.A. Pitt

P6 Protein phosphorylation and intracellular signalling networks in the postgenomic era

T. Hunter

P7 The future - putting Humpty-Dumpty back together again

Denis Noble

P8 The Alliance for Cellular Signaling

Alfred G. Gilman

P9 Communication: from Neurons to People; from Present to Future

Steven P R Rose

A2 Desensitization of oncogenic receptor tyrosine kinases: opportunities for cancer therapy

Y. Yarden

A3 Vascular endothelial growth factor signal transduction

L. Claesson-Welsh

A4 Scatter Factor Receptors And Plexins: A Dynamic Receptosome

Paolo M. Comoglio

A5 Fish, a multi-domain adaptor protein and Src substrate.

SA Courtneidge, D Seals E Azucena D Salinsky

B1 Interplay between Small GTPase signalling pathways in malignant transformation.

C.J.Marshall

B2 SRF: LINKING THE CYTOSKELETON TO TRANSCRIPTION

R.H. Treisman, J. Copeland, R. Grosse, F. Miralles, K. Murai, G. Posern

B3 Detection, Signalling and Repair of DNA Damage

S.P. Jackson

B4 Regulation of p53 function

Karen Vousden

B5 How chromosomal passengers INCENP, Aurora-B and Survivin regulate mitotic events

William C. Earnshaw, Ana Carvalho and Sally P. Wheatley.

C3 Interactions of poxviruses with the host cell

G. L. Smith

C5 Regulation of type III-secreted proteins in enterohaemorrhagic Escherichia coli O157:H7

D. L. Gally and A. J. Roe

C6 Yersinia pestis and plague

R.W. Titball, J. Hill, D. Lawton and K. Brown

C7 Expression of Borrelia Proteins in Ticks and Mammals

T.G. Schwan

C8 And the world fell out of his bottom!

C.A.Hart

D2 Agonist Binding To Peptide Hormone Receptors

M. Wheatley

D3 Mechanisms of GPCR Trafficking

J. L. Benovic

D4 Role of G protein-coupled receptor oligomerization in traficking and signalling

M.Bouvier

D5 G Protein-Coupled Receptor Regulatory Mechanisms: Basic and Clinical Implications

M.G. Caron

E2 Exploring Hedgehog Signaling in Mammals

Andy McMahon

E3 Control of cell number during axon guidance

A. Hidalgo

E4 Coordination of Organ Growth through Signalling in Plants

E.S.Coen, A.G. Rolland, J.A.Bangham, S.Impey.

E5 Genetic Analysis of Muscle Development in the Zebrafish

P W Ingham

F1 Signalling via PI3ks

P.T. Hawkins, J. Appleby, A. Condliffe, K. Davidson, C. Ellson, J. Ferguson, K. Hill, S. Krugmann, V. Paranavitane, N. Risso, S. Suire, H. Welch, L.Stephens

F2 3-phosphoinositide-mediated regulation of EGF receptor trafficking - the role of PX domain-containing proteins..

J. Carlton, G.E. Cozier and P.J. Cullen

F3 A role for PI3-kinase activity in adenovirus infection

D. Stokoe, K. Klupsch, F. McCormick, C O'Shea.

F4 Raf and Akt cooperate to promote cell cycle progression

M. McMahon, J. Roberts, A. Mirza

F5 Rap1 and Ral in Ras-mediated signal transduction

Johannes L. Bos

F6 Signals controlling transcription and apoptosis

A. Brunet, H. Tran, S.R. Datta, S.E. Ross, M.E. Greenberg

F7 Molecular Organization of Cytoskeletal Transduction Complexes

Scott Soderling, Kathleen Bins, Tony Pawson and John D. Scott

F8 Permissive signals act through PKC to control cellular responsiveness.

Johanna Ivaska, Richard, D.H. Whelan, Scott Parkinson, Philip Whitehead, Peter J. Parker

F9 Regulation of cell proliferation, transformation and survival by RAS signalling pathways

J.Downward

F10 Phospholipase D1 and potential targets of its hydrolysis product, phosphatidic acid.

N.T.Ktistakis

G1 Structure and Function of the Estrogen Receptor

R.E.Hubbard

G3 GENETIC DISSECTION OF NUCLEAR HORMONE RECEPTOR FUNCTIONS IN MICE

Erich F. Greiner, Francois Tronche, Christoph Kellendonk, Tim M. Wintermantel, Holger M. Reichardt, Thorsten Umland, and GŸnther SchŸtz

G4 Histone modifications and glucocorticoid actions

I.M. Adcock

G5 Cross-coupling between oestrogen receptor and PI3-kinase signalling

J.K. Liao

H1 The sense of smell: recognition and transduction of olfactory signals

Heinz Breer

H2 RODENT PHEROMONES-STRUCTURES, BIOCHEMICAL ASPECTS AND VOMERONASAL RESPONSES

M. V. Novotny

H3 Plant stress signalling: a long term view of how we might exploit plant/plant interactions

J.A. Pickett

H4 Arthropod semiochemicals: sealice and biting flies

A J Mordue(Luntz)

H5 Odor maps in the mammalian olfactory bulb

K. Mori

H6 The olfactory adventures of elephantine pheromones

L. E. L. (Bets) Rasmussen

H7 The Multiple Roles of Mouse Major Urinary Proteins

R J Beynon, J L Hurst

H8 Chemical Signaling in Mice

Gary K. Beauchamp

H9 Towards an understanding of the pregnancy blocking urinary chemosignals of mice

P. A. Brennan, P. Peele

I2 Macromolecules sneaking through nuclear pores and visualized with atomic force microscopy: Role of calcium

H. Oberleithner, C. Schafer, V. Shahin, L. Albermann

I3 NAADP-induced Ca²⁺ release from the nuclear envelope of pancreatic acinar cells

J.V. Gerasimenko, O.V. Gerasimenko, Y. Maruyama, A.V. Tepikin, and O.H. Petersen

I4 Calcium signalling in and around the nucleus of pancreatic acinar cells.

J. Gerasimenko, O. Gerasimenko, O. H. Petersen, N. Dolman, M. Ashby and A. Tepikin.

I5 Phosphorylation of cofilin by MPF activated in the nucleus modulates the InsP3 receptors

L. Santella, E. Ercolano, D. Lim, Gilda A. Nusco and F. Moccia

Poster sessions

Wednesday 4 September

Signalling through the Membrane
Tyrosine kinases

1 Insulin induces IRS-1 phosphorylation on three serine residues through different pathways

J.-F. Tanti, P. Gual, T. Gremeaux, T. Gonzalez and Y. Le Marchand-Brustel

2 Heteroligomerization of the endothelial Tie receptors

M.B. Marron and N.P.J. Brindle

3 The gamma subunit of retinal photoreceptor phosphodiesterase 6 is a novel intermediate regulating p42/p44 MAPK signalling in HEK 293 cells

K.F. Wan and N.J. Pyne

4 REPS2/POB1 inhibits EGF receptor signal transduction and internalization in prostate cancer cells

J.K. Oosterhoff, F. Penninkhof, A.O. Brinkmann, J.A. Grootegoed and L.J. Blok

5 Acceleration of non-radioactive HTS kinase assay development: rapid identification of kinase substrate and compatible phospho-specific antibodies by peptide micro-array technology

L. Dong, U. Hoffmueller, M. Melnick and M. Schutkowski

6 Regulation of MAPK pathway and Met receptor activation by HGF/SF in HaCaT keratinocytes

S.J. Payne, M. Delehedde, M. Lyon, T.J. McDonnell and D.G. Fernig

7 Insulin-stimulated NO-dependent cGMP production in endothelial cells is mediated by pertussis toxin sensitive G-protein and MAPK activation

O. Konopatskaya, A.C. Shore, J.E. Tooke and J.L Whatmore

8 Neurturin but not glial cell line-derived neurotrophic factor stimulates neuronal differentiation in NG 108-15 cells

R.H.K. Lee, W.L. Wong and S.Y. Chan

9 Use of cell-permeant peptide mimetics to study EGF-activated EGF receptor signalling in A431 cells

S.J. Hamilton, J.L. Eastwood and J. Nelson

10 Alopecia in transgenic mice over-expressing epidermal growth factor in skin

K.K.L. Mak and S.Y. Chan

11 Metastasis suppressor, tetraspanin, CD82/KAI1 is a new regulator of EGFR signalling

E. Odintsova, J. Voortman and F. Berditchevski

12 Regulation of endocytic traffic by Rho family GTPases

M. Wherlock, A. Gampel and H. Mellor

13 Effect of an inhibitor of diacylglycerol kinase and phorbol ester on cytosolic free Ca²⁺

I. Marrero and A. Déniz

14 Tamoxifen exhibits agonistic effects on cell cycle progression in anti-hormonal resistant breast cancer

F.E. Boyns, J.M.W. Gee and R.I. Nicholson

15 Involvement of HIF-1 in expression of VEGF induced by growth factors

C. Treins, S. Giorgetti-peraldi, J. Murdaca, G.L. Semenza and E. Van Obberghen

16 SOCS-3 and insulin resistance

B. Emanuelli, P. Peraldi, C. Filloux, C. Chavey, K. Freidinger, D.J. Hilton, G.S. Hotamisligil and E. Van Obberghen

17 The glycoprotein VI cytoplasmic domain provides a scaffold for assembly of a signalling protein complex

C.M. Elton, C.G. Knight and R.W. Farndale

18 Activation of IGF-1 receptor kinase domain: what does dimerization do?

K. Baer, K. Schmolz, M. Gompert and H.W. Klein

19 Increased FAK phosphorylation and cell spreading on collagen of immortalized brain endothelial cells overexpressing the adaptor protein Shb

K. Holmqvist, M. Cross and M. Welsh

20 The Shb adaptor protein binds to tyrosine 766 in the FGFR-1 and regulates the Ras/MEK/MAPK pathway in endothelial cells

M.J. Cross, L. Lu, P. Magnusson, D. Nyqvist, K. Holmqvist, M. Welsh and L. Claesson-Welsh

21 Poster withdrawn

22 Semaphorin 4D induces invasive growth through activation of the Met tyrosine kinase receptor

S. Giordano, S. Corso, P. Conrotto, S. Artigiani, G. Gilestro, D. Barberis, L. Tamagnone and P. Comoglio

23 Application of single probe microscopy techniques in the study of FGF–2 HS interaction

R.Vidyasagar, T. Rudd, D.G.Fernig and R.J. Nichols

Cell Fate
Cell proliferation/cell death

24 PC depletion induces expression of the ER stress- and apoptosis-related protein CHOP/GADD153 and a decrease in the DAG/ceramide ratio

M.H.M. van der Sanden, M. Houweling, L.M.G. van Golde and A.B. Vaandrager

25 Novel arylethylene derivatives as potential anticancer agents: mode of action

E. Levy, Z. Rappoport, O. Shpilberg and I. Nathan

26 Selective activation of the c-Jun N-terminal kinase (JNK) pathway fails to elicit apoptosis unless the phosphoinositide-3-kinase (PI3K) pathway is inhibited

S.A. Molton, D.E. Todd and S.J. Cook

27 Effects of Ruk on cell survival

E.B. Borthwick, G. Middleton, C.T. Luke and V.L. Buchman

28 Disruption of 14-3-3 dimers by phosphorylation

J. Murphy, A.F. Lopez and J.M. Woodcock

29 Activation of the ERK Pathway by DRaf-1:ER* represses serum-withdrawal-induced Bim expression and cell death

K. Balmanno, C. Weston, C. Chalmers, S. Molton, R. Ley and S. Cook

30 Caspase sensitivity of TNF receptor signalling

D.J. MacEwan, A.A.A. Mohamed, S.J. Tucker and S.M. McFarlane

31 Tumour necrosis factor-a (TNF)-induced activation of c-Jun transcription factor is suppressed by inhibitors of caspases-3 and -6

S.J. Tucker and D.J. MacEwan

32 Role of JNK activation in the TNF-induced expression of cellular adhesion molecules in human umbilical endothelial cells

M.C. Connell and D.J. MacEwan

33 Alteration of TNF receptor levels and the effect on TNF-induced apoptosis of TF-1 human leukaemia cells

C. Rae and D.J. MacEwan

34 RIP controls TNF-induced life and death in human eythroleukaemia cells

A.F. Littlejohn, C. Rae and D.J. MacEwan

35 TNF-induced cell death and the role of c-Jun N-terminal kinase and caspases

A.A.A. Mohamed and D.J. MacEwan

36 Inhibition of IGF signalling caused by caspase 10 cleavage of IRS during epithelial apoptosis

K.A. Green and C.H. Streuli

37 The human NUDT12 and Mouse Nudt13 Nudix hydrolase genes encode NADH pyrophosphatases

S.R. AbdelRaheim and A.G. McLennan

38 Effects of reactive nitrogen species on protein S-nitrosylation and apoptosis in pancreatic b-cells

B.S. Hosker, R.F. Bilton, G.M. Lowe and A.C. Loweth

39 PKCeta associates with cyclin E/Cdk2 complex in serum-starved MCF7 and NIH3T3 cells

M. Shtutman, T. Hershko, A. Maissel, E. Fima and E. Livneh

40 FGF-2 induction of the proliferation of Rama 27 fibroblasts requires both MAPK and NFkB pathways but is independent of transient activation of PI3K/AKT

R. Borgo, H.-Y. Zhu, S. Payne, P.S. Rudland, C.D. Green, D.G. Fernig and M. Delehedde

41 Identification of a Novel p53-Ser20 kinase cascade in undamaged cells that is modulated by chemical oxidants

A.L. Craig, L.R. Burch, A. Smith, D. Dornan and T.R. Hupp

42 MDM2 and MTBP. Another feedback loop that regulates p53?

N. Vlatkovic, M. Brady, D.M. Gore, D. Spiller, M.R.H. White, A. Dodson and M.T. Boyd

43 Rac is involved in the apoptotic response of N1E-115 neuroblastoma cells to staurosporine, but not to ceramide

M. Kozma, R. Kozma, G. Pirianov, A. Best, L. Lim and H. Mehmet

Thursday 5 September

Signalling in Host-pathogen Interactions

44 Time-course induction of apoptosis by wild-type and attenuated rubella virus strains, and sequencing of their non-structural genes

S. Cooray, L. Jin and J.M. Best

45 Characterization of Nramp1 regulation

H. Bowen, T.E. Biggs, E. Phillips, V.H. Perry, D.A. Mann and C.H. Barton

46 Regulation of dinucleoside polyphosphates is required for cellular invasion by Salmonella typhimurium

T. Ismail, C.A. Hart and A.G. McLennan

47 The African swine fever virus A238L protein: an inhibitor of calcineurin phosphatase and of NF-kB activation

G.C. Bowick, R.N. Silk, S. Najjam, J.E. Miskin, L.C. Goatley, C.C. Abrams and L.K. Dixon

48 Stimulation of stress-activated mitogen activated protein kinase (MAP kinase) in HEP-2 cells by enteropathogenic Escherichia coli (EPEC)

A.M. Graham, M. Rizwan, A.B. Alzahrani, L.J. Crowther and J.N. Fletcher

49 The secreted EspB protein of enteropathogenic Escherichia coli is responsible for tyrosine phosphorylation of a 78kDa host protein

J.N. Fletcher, L.J. Crowther and A.M. Graham

Signalling through the Membrane
G-protein-coupled receptors

50 Alterations in myocardial adenylyl cyclase signalling induced by chronic hypoxia

J. Novotny, M. Hrbasova, F. Kolar and P. Svoboda

51 The role of the extracellular face of the rat vasopressin V1a receptor

J. Simms, S.R. Hawtin, R.A. Parslow, C. Argent, Z. Lawson, M. Conner and M. Wheatley

52 Novel mode of inhibition of adenylyl cyclase by calmidazolium

A. Haunsø, J. Simpson and F.A. Antoni

53 Evidence suggesting a role for GPCR LPHH1 in human cancer

S. Lake, S. Brennand, M. Leversha, T. Knapp, J. Field, J. Hayflick and J. Heighway

54 Development of a generic dual reporter gene assay

T.C. Kent, K.S.J. Thompson and L.H. Naylor

55 Functional responses to neurohypophysial peptides and kinins in a novel endothelial cell line

S.J. Payne, S. Jones, M.J. Farquhar, C.A. Brown, B. Alexander and J. Howl

56 Characterization of the histamine-induced internalization of a myc/GFP-tagged human histamine H1 receptor

S.M. Oakley, T.J. Self and S.J. Hill

57 GRK phosphorylation modulates the kinetics of PTH receptor-arrestin interaction

C. Krasel, M. Bünemann, J.-P. Vilardaga and M.J. Lohse

58 Analysis of G-protein-coupled receptor phosphorylation sites

A.B. Tobin and A. Blaukat

59 Adrenomedullin (AM) selectivity over calcitonin gene-related peptide (CGRP) is determined by the extreme N-terminus of the calcitonin receptor-like (CL) receptor

K. Husmann, D. Koller, L. Ittner, R. Muff, J.A. Fischer and W. Born

60 Quantal Ca²⁺ release and enhanced phospholipase C activity account for CXCR2-mediated intracellular Ca²⁺ responses in the presence of P2Y2 receptor activation.

T.D. Werry, G.F. Wilkinson and G.B. Willars

61 Elucidation of CB₁ mediated signal transduction using proteomics and peptidomimetic probes

S. Jones and J. Howl

Cell Fate
Cell differentiation/development

62 Activin A induces Smad2, but not Smad3, through PKA in avian granulosa cells

B. Schmierer, M. Schuster, A. Shkumatava and K. Kuchler

63 Postsynaptic calcium signalling in skeletal muscle of zebrafish embryos during neuromuscular development

R. Ashworth

64 Finding a function for human RhoBTB2, a novel Rho GTPase

L.A. Skelton, S. Pellegrin and H.A. Mellor

65 Nuclear PLCb1 expression and activity is upregulated during 3T3-L1 adipocyte differentiation

S.J. O’Carroll, M.D. Mitchell and R.S. Gilmour

66 Wnt4 signalling prevents endothelial cell migration into the developing mouse ovary

K. Ward, J. Brennan, B. Capel and A. Swain

67 Signalling in developing vascularization of the urinary tract

S. de Silva Rudland, A. Platt-Higgins and P.S. Rudland

68 Zebrafish pancreatic islet development and assembly in vivo

L. Gnuegge, E. Ellertsdottir and W. Driever

69 LRP5&6/Arrow can signal independently of Frizzled

K. Brennan, J.M. Gonzalez Sancho, L.R. Howe, L.A. Castelo-Soccio and A.M.C. Brown

70 Cell-specific requirement of heparan sulphate proteoglycans for extracellular movement of Wingless

L.S. Gramates, G.-H. Baeg and S. Cumberledge

71 Pyst-1/MKP3: a question of self-control

J.S. Lunn, R.J. Dickinson, S.M. Keyse, A. Munsterberg and K.G. Storey

Friday 6 September

Signalling through the Cytoplasm
Lipid signals and kinase cascades

72 Essential role of PDK1 in regulating cell size and development

M.A. Lawlor, A. Mora, P.R. Ashby, M.R. Williams, V. Murray-Tait, L. Malone, A.R. Prescott, J.M. Lucocq and D. Alessi

73 Novel insights into the activation of smooth muscle contraction by Ca²⁺ and calmodulin

D.P. Wilson, C. Sutherland and M.P. Walsh

74 Regulation of subcellular localization of PRK1 by Ser-377 phosphorylation

Y. Zhu and W. Duan

75 P-Rex1, a novel PtdIns(3,4,5)P3- and Gbg-regulated guanine-nucleotide exchange factor for Rac

H.C.E. Welch, J. Coadwell, C.D. Ellson, G.J. Ferguson, S.R. Andrews, H. Erdjument-Bromage, P. Tempst, P.T. Hawkins and L.R. Stephens

76 Regulation of COX-2 and PGE2 production in human endometrial cancer cells through PI 3-K/Akt pathway

E. Asselin, M.E. St-Germain, V. Gagnon and S. Parent

77 Influence of PTEN mutation on Akt and Bcl-2 in human endometrial cancer cells

S. Parent, V. Gagnon, M.E. St-Germain and E. Asselin

78 Analysis of the interactions of the N- and C-terminal PH domains of centaurin-a₁ with inositol lipids

K.G. Brandom and K. Venkateswarlu

79 An occludin kinase in brain extracts: identification as CK2

C. Smales, M. Ellis, R. Baumber, N. Hussain, H.P. Desmond and J.M. Staddon

80 Detection and stimulation of a mitogen-activated protein kinase in hemocytes from Lymnaea stagnalis

L.D. Plows, A.J. Davies, R.T. Cook and A.J. Walker

81 ARAP3, a PI3K-regulated link between Arf and Rho family GTPases

S. Krugmann, K.E. Anderson, N.Risso, A.B. Holmes, L. Stephens and P.T. Hawkins

82 The kin-1 cAMP-dependent protein kinase in the nematode C. elegans

L.C. Bowen, M. Tabish, R.A. Clegg, H.H. Rees and M.J. Fisher

83 An X-chromosome-encoded cyclic AMP-dependent protein kinase (PK-A) catalytic subunit in the nematode, C. elegans

A.V. Bicknell, M. Tabish, R.A. Clegg, H.H. Rees and M.J. Fisher

84 Concentration of Syntaxin4 and SNAP23 in lubrol-insensitive lipid raft microdomains in adipocytes: implications for insulin-regulated trafficking of GLUT4

E. Manickam, C. Mitchell, F. Ng and T. Rowe

85 Identification of a novel domain in two mammalian inositol polyphosphate 5-phosphatases that mediates membrane ruffle localization

A. Tan, R. Gurung, M. McGrath, R. Huysmans, A. Munday, M. Prescott, L. Ooms, J. Whisstock and C. Mitchell

86 Purification and co-expression of an active Cdk5/Nck5a p25 complex using the Baculovirus expression system

P. Carter, G. Pettman, A. Bridges, D. Mills and C. Lewis

87 The regulation of ERK signalling pathways via a cyclic AMP/Rap1-dependent and protein kinase A-independent mechanism in CHO cells

R.H. Bhogal, R. Mistry, J.L. Blank and R.A.J. Challiss

88 Poster withdrawn

89 Regulation and function of protein phosphatase PP2A in chemokine signalling of leucocytes

M. Castelli, C. Gillieron, C. Weiss, C. Chabert, M. Camps and C. Rommel

90 Real-time analysis of calcium-dependent Ras deactivation by CAPRI

P.J. Lockyer, T. Bivona, M.R. Philips and P.J. Cullen

91 ESI-MS of cultured cell phosphatidylinositol compositions: surprises and implications for signalling

A.N. Hunt and A.D. Postle

92 Group I metabotropic glutamate receptor coupling to the extracellular signal-regulated kinase (ERK) pathway in Chinese hamster ovary (CHO) cells

S. Thandi, J.L. Blank and R.A.J. Challiss

93 Nudix hydrolases in Drosophila melanogaster

L.J. Winward, W.G.F. Whitfield and S.T. Safrany

94 TPIP: a novel phosphoinositide 3-phosphatase

S.M. Walker, C.P. Downes and N.R. Leslie

95 Effects of thrombin and histamine on the activation of eNOS, Akt and the forkhead transcription factor FKHR

B. Thors, H. Halldorsson and G. Thorgeirsson

96 Reduced PtdIns(3,4,5)₃ formation and PKB activation in mice lacking the p101 regulatory subunit of PI3Kg

A.M. Condliffe, G.J. Ferguson, E.R. Chilvers, M. Turner, P.T. Hawkins and L. Stephens

97 Nuclear localization of MKP-2 is controlled by two NLSs

C.M. Sloss, L. Cadalbert and R. Plevin

98 Shp-2 regulates LPS-stimulated NF-kB pathway and interacts with IKK

K. Forbes, R. Plevin and A. Paul

99 Noradrenaline stimulates phosphatidic acid production in lipid rafts/caveolae of intact small arteries

C.J. Clarke, V. Ohanian and J. Ohanian

100 Regulation of protein phosphorylation by higher inositol phosphates

L.S. Solyakov and A.B. Tobin

101 Sphingosine kinase and sphingosine 1-phosphate phosphatase: molecular tools to investigate sphingosine 1-phosphate signalling

D. Thompson, R. Tate and S. Pyne

102 Identification, localization and activation of HSP27 by noradrenaline in vascular smooth muscle

R. Srinivasan, J. Ohanian, R. Quinlan and V. Ohanian

103 A breast carcinoma cell line expressing neuropilin-1 and plexin A1 responds to semaphorin 3A

N.H. Chadborn and B.J. Eickholt

104 Suppression of Raf-1 activity by cAMP is achieved through multiple phosphorylation events and 14-3-3 binding

N. Dumaz and R. Marais

105 MSK1 and MSK2 regulate immediate early gene transcription in fibroblasts

G.R. Wiggin, A. Soloaga, J.M. Carr, V.A. Beardmore and J.S.C. Arthur

106 Insulin induces phosphatidylinositol-3-phosphate formation on plasma membrane

T. Maffucci and M. Falasca

107 Phosphorylation of Munc18/nSec1 regulates the kinetics of exocytosis

J.W. Barclay, T.J. Craig, R.J. Fisher, G.J.O. Evans, A. Morgan and R.D. Burgoyne

108 Characterization of BRAF mutants in human cancer

M. Garnett, R. Wooster, A. Futreal, M. Stratton, H. Paterson, C. Marshall and R. Marais

109 Studies on gene activation by Smad binding elements

A. Zachrisson, J. Hoerling and M. Luthman

110 ZONAB: a novel RalA effector that regulates ErbB-2 expression

P. Frankel, A. Aronheim, M. Balda, K. Matter and C.J. Marshall

Thursday 5 September

Signalling in the Nucleus
Nuclear receptors in endocrine systems

111 Regulation of ecdysteroid signalling

H. Takeuchi, J.-H. Chen, P.C. Turner and H.H. Rees

112 Functional differences between the two oestrogen receptors expressed in human breast cancer cells in culture

S.Y.M. Alakilli, R. Borgo and C.D. Green

113 Does protein kinase C play a role in acquisition of resistance to antioestrogenic therapies in breast cancer?

I. Lewis, J. Assender and R. Nicholson

114 Increased oestrogen receptor phosphorylation at serine 118 in tamoxifen-resistant MCF-7 breast cancer cells

D.J. Britton, I.R. Hutcheson, J.M.W. Gee, D. Barrow and R.I. Nicholson

115 Differential modulation of transcriptional activity of oestrogen receptors by direct interactions with T cell factors

M.K. El-Tanani, D. Liu, D.G. Fernig, B.R. Barraclough, C.D. Green and P.S. Rudland

Friday 6 September

Biochemistry and Behaviour
Chemical signalling between individuals

116 Characterization of bank vole urinary proteins

M. Turton, J.L. Hurst, D.H.L. Robertson and R.J. Beynon

117 The protein complement of pig saliva

S.A. Cheetham, M. Mendl, R.J. Beynon, D.H.L. Robertson, J.R. Hayter and J.L. Hurst

118 Identification of putative thiol receptors in the mammalian olfactory epithelium

R.H. Spice, R.J. Beynon and K.C. Persaud

119 A mouse major urinary protein that preferentially binds 2-sec-butyl-4,5-dihydrothiazole

S.D. Armstrong, D.H.L. Robertson, J.L. Hurst and R.J. Beynon

Signalling in the Nucleus
Nuclear calcium signalling

120 Functional and genomic analysis of weak acid stress response in yeast

C. Schüller, Y.M. Mamnun, B.E. Bauer, M. Mollapour, G. Krapf, C. Gregori, P. Piper and K. Kuchler

121 Differential control of K⁺ channels by Ca²⁺ from different sources in chromaffin cells

D.J. Wallace and P.D. Marley

122 The development of a mammalian gene delivery vehicle for artificial chromosome constructs

M. Howard and J. Quinn

123 Patch-clamp studies of plasma membrane anion channels associated with overexpression of the organellar chloride intracellular channel protein CLIC4 (p64H1)

I.Proutski, N. Karoulias and R.H. Ashley

124 Molecular regulation of the sphingosine-1-phosphate receptor EDG3

F.U. Ord-Shrimpton and T.M. Palmer

125 Xanthine oxidoreductase inhibition and possibility of cytokine bone resorption suppression

M. Benboubetra, L. Arrar, N. Al-Muhtaseb and A. Baghiani

126 Inhibition of cell cycle progression in human cancer cell lines by g-tocopherol is associated with reduction of cyclin levels

R. Gysin, A. Azzi and T. Visarius

127 Adhesion receptor signalling to 5-lipoxygenase (5-LO) compartmentalization and activation

G.F. Sud’ina, T. Brock, M.A. Pushkareva, S.I. Galkina, D.V. Turutin, M. Peters-Golden and V. Ullrich

128 Superoxide inactivates ecto-ATPase and stimulates polymorphonuclear (PMN) attachment to endothelial cells

M.A. Pushkareva and G.F. Sud’ina

129 Signalling by integral plasma membrane Ser/Thr kinases

W. Duan and Y. Zhu

130 Cytosolic multiple inositol polyphosphate phosphatase rescues the glucose-induced calcium response in a pancreatic beta-cell line

C.J. Barker, J. Yu, B. Leibiger, S.-N. Yang, J. J. Caffery, S.B. Shears, I. Leibiger and P.-O. Berggren

131 A reappraisal of 3-phosphorylated lipids in insulin-secreting cells

J. Yu, P.-O. Berggren and C.J. Barker

132 Agonists of protease-activated receptor-2 induce activation of NF kappa B and upregulation of cell adhesion molecules in human dermal microvascular endothelial cells

V. Shpacovitch, G. Varga, T. Brzoska, J. Buddenkotte, C. Stroh, C. Sommerhoff, K. Schulze-Osthoff, T.A. Luger and M. Steinhoff

133 Long-term activation of ERK 1/2 by serum or apigenin in HeLa cells lead to different cell fate

F. Llorens, F. Miro, N. Roher, L. Garcia, M. Plana, N. Gomez and E. Itarte

134 Two surfaces used for the study of receptor–ligand interactions

T. Rudd, R. Vidyasagar, D.G Fernig and R.J Nichols

135 Non-radioactive methods for the assay of PI 3-kinase and determination of mass of signalling lipids

A.Gray and C.P. Downes

136 Activation of protein kinase C in human platelets

M.J. Stafford, D. Cantrell, S.P. Watson and C.J. Pears

137 Definition of the signal transduction pathways involved in aberrant neuronal gene expression associated with epilepsy

K. Haddley and J. Quinn

138 Deciphering the role of the tachykinin pathway in anxiety and depression by the generation of a humanized mouse model for the NK1 receptor

S. Vasiliou, A. MacKenzie and .P. Quinn

139 Identification of YB-1 and regulatory interactions with the serotonin transporter VNTR

A. Scott, E. Lovejoy and J. Quinn

140 Characterization and localization of CD38 in murine testis

Z. Lu and C.F. Chang

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