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Ribosome Profiling Workshop

27 October 2016

Charles Darwin House, London, UK

Aim: To exchange expertise in both the experiment and bioinformatic analysis of ribosome profiling.


Ribosome profiling is still a relatively new technique, many people are trying to perform these types of experiments but so far there are only a small number of groups that have successfully published results using it. Therefore there are currently no standard analysis pipelines in the field. PhD students and postdocs spend a great deal of time trouble shooting both the experiments and analysis. This workshop will be beneficial to them and the translation field as an opportunity to share and discuss different approaches and problems. The workshop will provide an excellent training platform at all levels; student, postdoc, PI. The networking will help generate collaborations and opportunities for knowledge exchange.

The one day event will be a combination of training and round-table discussions. Many scientists are trying to use these methods and a discussion of what experts in the field are doing and alternative experimental and bioinformatic pipelines will allow scientists to learn about the tools available and share experiences of data analysis. This will be ideal for those just starting to plan ribosome profiling experiments and those in the middle of doing/analysing them.

Julie Aspden (University of Leeds, United Kingdom)
Ian Brierley (University of Cambridge, United Kingdom)
Juan Mata (University of Cambridge, United Kingdom)
Betty Chung (University of Cambridge, United Kingdom)
Thomas Hardcastle (University of Cambridge, United Kingdom)
Thursday 27 October 2016
08:30 - 09:00 Registration

09:00 - 09:45
General Overview of Ribosome Profiling
Olivier Namy (University of Paris-Sud, France) & Julie Aspden (University of Leeds)
09:45 - 10:45
Experimental Design and Experimental Variables
Marieke von Lindern (Sanquin Research and Lansteiner Laboratory) & Betty Chung (University of Cambridge)
10:45 - 11:15 Refreshment Break

11:15 - 12:30
Round Table Discussion on Methodological Issues
Andrew Firth & Ian Brierley (University of Cambridge, UK)
12:30 - 13:30 Lunch & Networking

13:30 - 14:30
Discussion of Methods
13:30 - 14:30
Overview / initial steps (processing, formats, etc - up to mapping)
Juan Mata (University of Cambridge, United Kingdom)
13:30 - 14:30
Graphical Analysis of ribosome profiling data (i.e. metagene profiles, meta-footprint profiles, subcodon profiles)
Pavel Baranov (University College Cork, Cork, Ireland)
13:30 - 14:30
Overview of available tools
Thomas Hardcastle (University of Cambridge, United Kingdom)
14:30 - 15:30
Introduction and demonstration of
Pavel Baranov (University College Cork, Ireland)
15:30 - 16:00 Refreshment Break
Sponsored by Merck Group

16:00 - 17:00
Workstation Demos/Q&A
Thomas Hardcastle (University of Cambridge, United Kingdom)
17:00 - 17:30
Summary of Discussion & Closing Comments
Conference Organizers
17:30 - 18:30 Drinks Reception