3rd UK workshop on membrane proteins: solubilisation and biophysical characterisation

4-6 April 2022
Aston University, Birmingham

Earlybird registration deadline: 4 February 2022 *FULLY BOOKED*

To join the waitlist for the 3rd UK workshop on membrane proteins please email conferences@biochemistry.org 

This three-day training event for early career researchers will focus on the function on biological membranes and on the biophysical techniques available to study their components in the context of biology and biotechnology. The event will comprise lectures, computer and laboratory-based practical sessions. Participants will leave the event with new knowledge and protocols that will allow them to use biophysical techniques for studying components of biological membranes in their research.

Topics include:

  • High throughput membrane protein expression
  • Solubilisation strategies
  • Membrane protein purification
  • Structural determination via circular dichroism, crystallography and EM
  • Lipid nanoparticles and their analysis.
  • Lipidomics via mass spectroscopy
  • Biophysical analyses including NMR and neutron scattering

Event sponsors:

    Nanotemper    

Event Programme

 Day One - Monday 4 April 2022 - Solubilisation of membrane proteins
 12:45 - 13:00  Registration
 13:00 - 13:45  Lecture 1: High throughput membrane protein expression
 Andrew Quigley, Diamond Light Source
 13:45 - 14:30  Lecture 2: Membrane protein solubilisation: detergents, SMALPs, nanodiscs and
 amphipols

 Vincent Postis, University of Leeds
 14:30 - 15:15  Lecture 3A: Protein purification
 Andrew Quigley, Diamond Light Source
 15:15 - 15:45  Refreshment Break
 15:45 - 17:45  Practical 1A: Lipid nanoparticles (SMALPs)
 Vincent Postis, University of Leeds
 Alice Rothnie, Aston University
 17:45 - 18:15  Lecture 3B: Mass Photometry: Measuring Molecules with Light
 Colin Grant, Refeyn
 19:00  Networking Drinks
 Day Two - Tuesday 5 April 2022 - Membrane Protein Structure
 09:00 - 09:45  Lecture 4: Circular dichroism of membrane proteins
 Bonnie Wallace, Birkbeck
 09:45 - 10:30  Lecture 5: Membrane protein crystallography
 Isabel Moraes, NPL
 10:30 - 11:00  Refreshment Break 
 11:00 - 12:30  Practical 1B: Lipid nanoparticles
 Vincent Postis, University of Leeds
 Alan Goddard, Aston University
 Alice Rothnie, Aston University
 Megan Cox, Aston University/Meritics Ltd.
 12:30 - 13:30  Lunch
 13:30 - 14:15  Lecture 6: Single particle CryoEM for membrane proteins structure
 Joshua White, University of Leeds
 14:15 - 15:00  Lecture 7: Single Molecule fluorescence
 Alan Goddard, Aston University
 15:00 - 15:30  Refreshment Break
 15:30 - 16:15  Lecture 8: Mass spec - lipidomics
 Corinne Spickett, Aston University
 16:15 - 17:30  Mass Spec Demo
 19:00  Conference Dinner
 Day Three - Wednesday 6 April 2022 - Biophysical and functional characterisation
 09:00 - 09:45  Lecture 9: MD of membrane proteins
 John Simms, Aston University
 09:45 - 10:30  Lecture 10: NMR of membrane proteins
 Boyan Bonev, University of Nottingham
 10:30 - 11:15  Lecture 11: Neuron techniques
 'Unravelling the Structural Complexity of Protein-Lipid Interactions with Neutron
 Reflectometry' - Luke Clifton, STFC
 11:15  Event Close

There are a variety of bursaries available for this meeting. Click here to find out more. 

Not a member of the Biochemical Society? Join today and save up to £100 on your registration fee.

Click here to download the event flyer.


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