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Annual Symposium - Frontiers in biological catalysis

10—12 January 2012

Robinson College Cambridge, UK

Poster session

P001 Allosteric regulation of the biotin-dependent enzyme, pyruvate carboxylase, by acetyl coenzyme A
Paul V Attwood, Abdussalam Adina-Zada, Tonya N Zeczycki, Martin St. Maurice, Sarawut Jitrapakdee, John C Wallace, W. W Cleland
P002 Vectorial electron transfer driven by enzyme motion
Christopher R Pudney, Basile Khara, Linus O Johannissen, Nigel S Scrutton
P003 Targeting Mycobacterium tuberculosis cytochrome P450 enzymes using fragment-based drug discovery
Sean A Hudson, Kirsty J McLean, David Leys, Andrew W Munro, Alessio Ciulli, Chris Abell
P004 The elucidation of the anaerobic biosynthesis of vitamin B12
Simon J Moore, Martin J Warren
P005 Structure and eEngineering of a compactin hydroxylase in pravastatin synthesis
Kirsty J McLean, David Leys, Marcus Hans, Andrew W Munro
P006 The birth of a novel function: robustness favours the native substrate above promiscuous enzyme activity
Narasimha Rao Uda, Marc Creus
P007 Production, purification and characterization of a thermo-alkalistable xylanase from a newly isolated Aspergillus fumigatus MA28
Bijender Kumar, Massarat Abbass
P008 Modulation of human galactokinase specificity: a question of flexibility
Helena Kristiansson, David J Timson
P009 Characterising phospholipase D activity in real-time using novel fluorescent assays
Mina-Olga Aletrari, David Metcalfe, Matthew Hodgkin, Graham Ladds
P010 Physico-chemical, kinetic and immunological properties of goat milk xanthine oxidoreductase
Djebbar Atmani
P011 Transition metal ions substitution on thermoalkalophilic lipase
Mohd Basyaruddin Abdul Rahman, Harmiza Harun, Roghayeh Abedikarjiban, Thean Chor Leow, Abu Bakar Salleh, Romas Kazlauskas
P012 Artificial metalloenzyme by introduction of new manganese binding site in T1 lipase
Mohd Basyaruddin Abdul Rahman, Mohd Zulhilmi Abdul Rahman, Thean Chor Leow, Raja Noor Zaliha Raja Abd. Rahman, Mahiran Basri, Abu Bakar Salleh
P013 Paracoccus complex I as a model for the bovine enzyme
Jamie Blaza, Judy Hirst
P014 Reversible methylation at DNA-cytosine-5-carbon by DNA-methyltransferase and possible mechanism of inhibition by 5-Aza-2'-deoxycytidine
SAMIR K PATRA, Moonmoon Deb, Dipta Sengupta, Swayamsiddha Kar, Arunima Silpi
P015 DNA binding studies on T5 5`flap endonuclease
Faizah A AlMalki, Jing Zhang, Claudia S Flemming, Svetlana Solovieva, Jon R Sayers, Peter J Artymiuk
P016 Partial purification and characterization of Exoglucanase (EC. from Aspergillus niger S.L.1 using corn cobs as a carbon source
Richard Auta, Stephen A James, Peter A Egwaikhide, Emmanuel M Bali
P017 Deciphering the function of YcbK lipoprotein-like hypothetical protein from Klebsiella pneumoniae MGH 78578
Boon Aun Teh, Sy Bing Choi, Mohd Nazalan Mohd Najimuddin, Abu Bakar Salleh, Habibah A Wahab, Normi Mohd Yahaya
P018 TREX1 dominant mutations in lupus and Aicardi-Goutieres syndrome
Jason M Fye, Clinton D Orebaugh, Stephanie R Coffin, Thomas Hollis, Fred W Perrino
P019 Tuning the selectivity of a Taxus cytochrome P450 monooxygenase
Vikramaditya G Yadav, Ajikumar P Kumaran, Chin G Lim, Gregory Stephanopoulos
P020 NQO1 and cancer: a question of stability
Clare F Megarity, David J Timson
P021 Chiral inversion of NSAID-CoA esters by human α-methylacyl-CoA racemase 1A
Timothy J Woodman, Pauline J Wood, Andrew S Thompson, Thomas J Hutchings, Georgina R Steel, Ping Jiao, Michael D Threadgill, Matthew D Lloyd
P022 Unexpected stereoselective exchange of straight-chain fatty acyl-CoA α-protons by human α-methylacyl-CoA racemase 1A (P504S)
Fouzia Sattar, Daniel J Darley, Francesco Politano, Timothy J Woodman, Michael D Threadgill, Matthew D Lloyd
P023 Kinetic studies of phenylalanine dehydrogenase from Bacillus sphaericus with a variety of substrates
Rajesh Narayanan, Paul C Engel
P024 Novel production system for the reductive dehalogenase PceA, an oxygen sensitive and membrane associated enzyme
Hanno Sjuts, Karl Fisher, Mark S Dunstan, David Leys
P025 Realising the biotechnological potential of a well characterised enzyme: generation and screening of a galactose oxidase library
Lucy A Chappell, W B Turnbull, Arwen R Pearson, Michael J McPherson
P026 Structural insights into the mechanism of acetolactate decarboxylase
Victoria Marlow, Dean Rea, Martin Wills, Vilmos Fülöp
P027 Metabolic enzymes- novel drug targets in Fasciola hepatica?
Veronika Zinsser, David J Timson, Elizabeth Hoey, Alan Trudgett
P028 Measurement of human aspartate aminotransferase isoenzymes in serum by immunoassay method
Hong-tao Wang, Hyun-jeong Kim, Stephanie Ho, Eui-yul Choi
P029 Towards the in vitro genenration of a starch granule
Ellis O'Neill, Abdul Rashid, Clare Stevenson, Anne-Claire Marquis, Martin Rejzek, Patrick Gunning, S Bornemann, David Lawson, Rob Field
P030 The structural basis of substrate specificity and regulatory behaviour in glutamate dehydrogenase from Clostridium symbiosum
Pratibha rani Gurrala
P031 Production of β-mannanase by local Aspergillus niger isolate, using natural substrates
Onyetugo C Amadi
P032 Mechanistic insights into human vascular adhesion protein-1 catalyzed primary amine oxidation
Dominic P Heuts, Jennet Gummadova, Jiayun Pang, Stephen E Rigby, Nigel S Scrutton
P033 A new enzyme, glucan pathway and anti-TB target
Stephen Bornemann
P034 Hydrolysis of ibuprofenoyl-CoA and other 2-APA-CoA esters by human Acyl-CoA thioesterases (ACOTS) 1 and 2
Xiao Qu, Angel Leung, Amanda C Allan, Grace Chui, Thomas J Hutchings, Ping Jiao, Lawrence Johnson, Wai Y Leung, Portia K Li, Georgina R Steel, Andrew S Thompson, Michael D Threadgill, Timothy J Woodman, Matthew D Lloyd
P035 Crystal and SAXS structures, enzyme kinetics, and stability studies to probe structure-function relationships of peroxisomal multifunctional enzyme type 2
Tuomo Glumoff, Maija Mehtälä, Tatu J Haataja, Clement Blanchet, Marc F Lensink, Dmitri I Svergun, J. K Hiltunen
P036 Activation of Aurora-A by TPX2 and phosphorylation are independent and synergistic
Charlotte A Dodson, Richard Bayliss
P037 Proton-coupled coupled electron transfer in the catalytic cycle of nitrite reductase
Nicole G Leferink, Derren J Heyes, robert r Eady, Samar S Hasnain, Nigel S Scrutton
P038 Prolyl-4-hydroxylase: Are conformational changes coupled to ligand binding?
James E Longbotham, Christopher R Pudney, Christoph Loenarz, Emily Flashman, Christopher J Schofield, Nigel S Scrutton
P039 A long-lost enzyme found: heterologous expression of SAMO
Mary Ortmayer, Pierre Lafite, Binuraj Menon, Andrew W Munro, David Leys
P040 MACiE (Mechanism, Annotation and Classification in Enzymes): Exploring diversity in biocatalysis
Sophie Williams, Nicholas Furnham, Janet M Thornton, Gemma L Holliday
P041 Kinetic study of amidase catalysed transamidation reactions in reverse micelles
Marisa Bernardo, Amin Karmali, Rita Pacheco
P042 Photogeneration of reactive oxygen species, antimicrobial activity and photoinduced DNA cleavage by 1,4-quinones
Yesu T Marian, Southamani Gurusamy, Gandhidasan Rathnasamy, Murugesan Ramachandran
P043 Evolution of NADH binding domain superfamily: sequence and chemical perspective
Sergio Martinez Cuesta, Nicholas Furnham, Janet M Thornton
P044 Purification and characterization of the reductive dehalogenase from Desulfitobacterium dehafniense
Karl Fisher, Mark Dunstan, Hanno Sjuts, Laura Kemp, David Leys
P045 Asymmetric synthesis of non-natural amino acids using E.coli branched chain aminotransferase
Xuejing Yu, Xingguo Wang, Paul C Engel
P046 Single enzyme transformation of flavin into dimethylbenzimidiazole
Hannah F Collins, Martin J Warren
P047 Characterisation of enzymes involved in the alternative haem biosynthesis pathway
David Palmer
P048 Targeting new processes to bacterial microcompartments
Sarah Newnham
P049 Fluorescence-based studies of the binding of an inhibitor of Hematopoietic Prostaglandin D synthase
Laura J Williams, Bernadette Mouzon, Nathalie Barrett, Anthony J Shillings, Robert V Leydon, Simon T Hodgson, Gordon Saxty, Jonathan P Hutchinson
P050 Nuclear quantum effects in the release pocket of bacteriorhodopsin
Ian M Grant, Sergei D Ivanov, Marcel Baer, Gerald Mathias, Dominik Marx
P051 Biochemical and biophysical characterization of a novel alcohol dehydrogenase from Candida parapsilosis ATCC 7330
nidhi aggarwal, Anju Chadha
P052 ALA synthase in disease: characterizing gain of function C-terminal deletions that lead to X-linked dominant protoporphyria
Dimitrios Ladakis
P053 The role of conserved active site residues within cytochrome c nitrite reductase
Colin Lockwood, Thomas A Clarke, Julea N Butt, David J Richardson
P054 FunTree: a resource for investigating the evolution of novel enzyme function and chemistry within structurally defined protein superfamilies
Nicholas Furnham, Ian Sillitoe, Gemma L Holliday, Alison L Cuff, Christine A Orengo, Janet M Thornton
P055 A novel rate law for surface-active enzymes: general properties and application to starch phosphorylation/dephosphorylation
Önder Kartal, Mahdi Hejazi, Joerg Fettke, Oliver Ebenhöh, Martin Steup
P056 Macromolecular crystallography at the European Spallation Source
Esko Oksanen
P057 Good vibrations in enzymatic H-transfer: the effect of pressure on barrier compression and isotope effects
Linus O Johannissen, Sam Hay, Nigel S Scrutton, Michael J Sutcliffe
P058 Identification of novel Cytochromes P450: redox partner fusion systems
Dominika Luciakova, Kirsty J McLean, Andrew W Munro