Quantitative Proteomics 2017

Discovery proteomics is now relatively straightforward, but the identification of a protein in a complex sample is immediately followed by quantitative questions – how much, how much does the abundance change?

 

However, there is a rather bewildering choice of approaches to quantitative proteomics. First, the discrimination between absolute and relative quantification, second, the choice of labelling or label-free, third, the selection of appropriate label, fourth the choice between metabolic and chemical labelling and finally, the selection of the most appropriate software for data analysis, the use of appropriate multivariate statistical methods and the importance of adherence to data standards in deposition and reporting of quantitative data.

 

The course was a substantially enhanced version of previous, highly successful iterations. There were more lectures and there were four small group sessions per participant.

 

This training day comprised a mixture of formal lectures, delivered by experts in the field, and a series of small group teaching sessions that cover specific aspects. The small group sessions had a maximum of 10 participants, and attendees attended four from a choice of 8-10.

 

By the end of the course, participants had gained a better understanding of the landscape of quantitative proteomics, have an appreciation of the breadth of approaches that can be adopted, and be introduced to the data analysis strategies that accompany quantitative proteomics experiments.

 

Organizers:
Rob Beynon (University of Liverpool, UK)
Victoria Harman (University of Liverpool, UK)
Monday 22 May 2017
10:00 – 11:00 Registration

11:00 – 11:15 Introduction and Welcome

 

Landscapes and strategies

Monday 22 May 2017
11:15 – 11:45
Introduction: An overview of quantitative proteomics workflows – experimental design, workflows and analysis
Rob Beynon (University of Liverpool, United Kingdom)
11:50 – 12:30
Considerations in experimental design and statistical analysis in quantitative proteomics
Kathryn Lilley (University of Cambridge, United Kingdom)
12:30 – 13:30 Lunch

13:30 – 14:05
Label-free or label mediated, relative or absolute?
Claire Eyers (Institute of Integrative Biology, United Kingdom)
14:05 – 14:15 Briefing and relocation to small classrooms

 

Selected topics in quantitative proteomics

Monday 22 May 2017
14:15 – 15:30
Small group session 1 (please see below)
15:30 – 15:45 Refreshment Break

15:45 – 17:00
Small group session 2 (please see below)
17:00 – 19:00 Ask the Experts
Drinks reception

 

Landscapes and strategies

Tuesday 23 May 2017
09:00 – 09:40
Bioinformatics for quantitative proteomics
Simon Hubbard (University of Manchester, United Kingdom)
09:40 – 10:30
Stable isotopes in quantitative proteomics
Rob Beynon (University of Liverpool, UK)
10:30 – 11:00 Refreshment Break

 

Selected topics in quantitative proteomics

Tuesday 23 May 2017
11:00 – 12:15
Small group session 3 (please see below)
12:15 – 13:00 Lunch

13:00 – 14:15
Small group session 4 (please see below)
14:15 – 14:30 Refreshment Break

 

Landscapes and strategies

Tuesday 23 May 2017
14:30 – 15:00
Design and implementation of a quantitative proteomics study
All instructors
15:00 Closing Remarks

Date

May 22 2017 - May 23 2017

Time

All Day
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