Seeing DNA repair and mutagenesis in bacteria
DNA repair and mutagenesis are essential for the stability and plasticity of genomes in all organisms. In this webinar, Dr Stephan Uphoff, from the University of Oxford, will describe the development of advanced fluorescence microscopy methods to observe protein function at the level of single molecules in individual bacterial cells. Dr Uphoff is the Biochemical Society’s 2020 Colworth Medal winner, which he received for his work in the area.
With these advanced fluorescence microscopy methods, it is possible to directly watch the movement of DNA repair proteins as they search for and fix DNA lesions. Microscopy can also be used to visualise sites of nascent mutations, and decipher which molecular mechanisms act in the creation or prevention of mutations. Ultimately, these techniques provide direct insight into the processes that help bacteria survive and adapt to stress conditions such as antibiotic treatments.
Join us at this webinar to hear more about the latest developments in DNA repair.
Invited speaker: Dr Stephan Uphoff, 2020 Colworth Medal winner
- Turning the Mre11/Rad50 DNA repair complex on its head: lessons from SMC protein hinges, dynamic coiled-coil movements and DNA loop-extrusion? >
- Bacterial phenotypic heterogeneity in DNA repair and mutagenesis >
- Visualizing the inner life of microbes: practices of multi-color single-molecule localization microscopy in microbiology >
- From single bacterial cell imaging towards in vivo single-molecule biochemistry studies >